Molecularbiological study on a novel organomercurial decomposing enzyme from iron-oxidizing bacterium Acidithiobacillus ferrooxidans and use of the enzyme for bioremediation of mercury
| Project/Area Number |
20580079
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Okayama University |
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Principal Investigator |
SUGIO Tsuyoshi Okayama University, 大学院・自然科学研究科, 教授 (20033269)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Fumiaki 岡山大学, 環境管理センター, 准教授 (90294446)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 鉄酸化細菌 / 水銀還元酵素 / 有機水銀分解酵素 / チトクロームcオキシダーゼ / Acidithiobacillus ferrooxidanas / iron-oxidizing bacterium / mercury / organomercurial / acidithiobacillus ferrooxidans / mercury reduction / mercury volatilization / mercury resistance / cytochrome c oxidase / mercury reductase / organomercury lyase / Acidithiobacillus ferrooxidans / iron-oxidizing bacteria / sulfur-oxidizing bacteria / cytochrome c oxiase / sulfur-oxidizing bacterium |
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| Research Abstract |
It has been known that iron-oxidizing bacterium Acidithiobacillus ferrooxidans has a mercuric reductase (MerA) which reduces Hg^<2+> to a volatilizable metal mercury (Hg^0), but not has a mercuric lyase (MerB) which cleaves a C-Hg bond in organomercurials. Author newly found a novel Hg^0 volatilizing enzyme system in a highly mercury resistant A. ferrooxidans MON-1. Namely, strain MON-1 has a mercury resistant aa3-type cytochrome c oxidase ( aa_3) and α- and β-subunits in aa_3 has activities to cleave C-Hg bond in organomercurials and to reduce Hg^<2+> to Hg^0.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Volatilization of metal mercury from organomercurials by highly mercuryresistant Acidithiobacillus ferrooxidans MON-1, Biosci.2010
Author(s)
Sugio, T., Komoda, T., Okazaki, Y., Takeda, Y., Nakamura, S., Takeuchi, F.
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Journal Title
Biotechnol. Biochem. Vol.74
Pages: 1007-1012
Related Report
Peer Reviewed
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[Journal Article] Volatilization of metal mercury from organomercurials by highly mercury-resistant Acidithiobacillus ferrooxidans MON-1.2010
Author(s)
Sugio, T., Komoda, T., Okazaki, Y., Takeda, Y., Nakamura, S., Takeuchi, F.
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Journal Title
Biosci.Biotechnol.Biochem.
Volume: 74
Pages: 1007-1012
NAID
Related Report
Peer Reviewed
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[Journal Article] Reduction of Hg^<2+> with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant Acidithiobacillus ferrooxidans strain, MON-1.2008
Author(s)
Sugio, T., Fujii, M., Ninomiya, Y., Kanao, T., Negishi, A., Takeuchi, F.
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Journal Title
Biosci. Biotechnol. Biochem. Vol.72
Pages: 1756-1763
NAID
Related Report
Peer Reviewed
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