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Development of vacuole-targeting antifungal chemotherapy for suicide induction

Research Project

Project/Area Number 20580083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOsaka City University

Principal Investigator

TANAKA Toshio  Osaka City University, 大学院・理学研究科, 教授 (10137185)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsamphotericin B / polymyxin B / 選択的抗真菌作用 / allicin / 液胞破壊 / 選択毒性 / Candida albicans / amphoterisin B / zwiebelane / 液胞破壞作用 / アリシン / アンホテリシンB / 抗真菌作用 / 液胞 / エルゴステロール / 出芽酵母 / ポリミキシンンB / タマネギ
Research Abstract

The vacuole-disruptive activities of amphotericin B and polymyxin B were evaluated as novel types of fungicidal actions, and their mechanisms have been partly elucidated. This study also found allicin from garlic and zwiebelan from onion as enhancers of vacuole-targeting fungicidal activities of these antifungal agents, and thus proposes enzymatic production of allicin as a substitute for its production by the chemical reaction. A system has been additionally proposed for practically applying the chemotherapy depending on the vacuole-targeting fungicidal activities.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (31 results)

All 2011 2010 2009 2008 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article] Enhancement effect of N-methyl-N"-dodecylguanidine on the vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans.2011

    • Author(s)
      Yutani, M. Bordihan, B., Ogita, A., Fujita, K., Tanaka T.
    • Journal Title

      J. Antibiot. in press

    • NAID

      10030639452

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alliinase from Ensifer adhaerence and its use for generation of fungicidal activity.2011

    • Author(s)
      Yutani, M., Taniguchi, H., Borjihan, H., Ogita, A., Fujita, K., Tanaka, T.
    • Journal Title

      AMB Express Vol.1

      Pages: 1-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dependence on vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae.2010

    • Author(s)
      Ogita, A., Yutani, M., Fujita, K., Tanaka, T.
    • Journal Title

      J. Antibiot. Vol.63

      Pages: 689-992

    • NAID

      10030710558

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cyclic organosulfur compound zwiebelane A from onion (Allium cepa) functions as an enhancer of polymyxin B in fungal vacuole disruption.2010

    • Author(s)
      Borjihan, B., Ogita A., Fujita, K., Doe, M., Tanaka, T.
    • Journal Title

      Planta Med. Vol.76

      Pages: 1-3

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted yeast vacuole disruption by polyene antibiotics with a macrocyclic lactone ring.2010

    • Author(s)
      Ogita, A, Fujita, K., Usuki, Y., Tanaka, T.
    • Journal Title

      Int.J.Antimicrobiol. Vol.35

      Pages: 85-92

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungus Candida albicans and its enhancement by allicin.2010

    • Author(s)
      Borjihan, H., Ogita, A., Fujita, K., Hirasawa, E., Tanaka, T.
    • Journal Title

      J. Antibiot. Vol.62

      Pages: 691-697

    • NAID

      10030637073

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cyclic organosulfur compound zwiebelane A from onion (Allium cepa) functions as an enhancer of polymyxin B in fungal vacuole disruption2010

    • Author(s)
      Borjihan, B., Ogita A., Fujita, K., Doe, M., Tanaka, T.
    • Journal Title

      Planta Medica

      Volume: 76 Pages: 1864-1866

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dependence on vacuole disruption and independence of potassium ion efflux in fungicidal activity induced by combination of amphotericin B and allicin against Saccharomyces cerevisiae.2010

    • Author(s)
      Ogita, A., Yutani, M., Fujita, K., Tanaka, T.
    • Journal Title

      J.Antibiotics

      Volume: 63 Pages: 689-692

    • NAID

      10030710558

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted yeast vacuole disruption by polyene antibiotics with a macrocyclic lactone ring2010

    • Author(s)
      Akira Ogita
    • Journal Title

      International Journal of Antimicrobial Agents 35

      Pages: 89-92

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salinomycin and citric acid in combination demonstrate bactericidal activity against Gram-negative bacteria.2009

    • Author(s)
      Ogita, A., Fujita, K., Tanaka, T.
    • Journal Title

      Annals Microbiol. Vol.53

      Pages: 611-614

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synergisitic fungicidal activities of polymyxin B and ionophores, and their dependence on direct disruptive action of polymyxin B on fungal vacuole.2009

    • Author(s)
      Ogita, A., Konishi, Y., Borjihan, B., Fujita, K., Tanaka, T.
    • Journal Title

      J. Antibiotics Vol.62

      Pages: 81-87

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of the fungicidal activity of amphotericin B by allicin : Effects on intracellular ergosterol trafficking.2009

    • Author(s)
      Ogita, A., Fujita, K., Tanaka, T.
    • Journal Title

      Planta Medica, Vol.75

      Pages: 222-226

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The vacuole-targeting fungicidal activity of amphotericin B against the pathogenic fungal Candida albicans and its enhancement by allicin2009

    • Author(s)
      Hasibagan Borjihan
    • Journal Title

      Journal of Antibiotic 62

      Pages: 691-697

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salinomycin and citric acid in combination demonstrate bactericidal activity against Gram-negative bacteria2009

    • Author(s)
      Akira Ogita
    • Journal Title

      Annals of Microbiology 59

      Pages: 611-614

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of the funglcidal activity of amphotericin B by allicin : Effects on intracellular ergosterol trafficking2009

    • Author(s)
      Ogita, A., Fujita, K., Tanaka, T
    • Journal Title

      Planta medica 75

      Pages: 222-225

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergisitic fungicidal activity of polymyxin B and ionophores, and their dependence on direct disruptive action of polymxinn B on fungal vacuole2009

    • Author(s)
      Ogita, A., Konishi, Y., Borjihan, B., Fujita, K., Tanaka, T
    • Journal Title

      Journal of Antibiotics 62

      Pages: 81-87

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ポリミキシンBの真菌液胞膜断片化作用に関与する細胞内因子2011

    • Author(s)
      バヤラン、キョウ琴琴、荻田亮、藤田憲一、田中俊雄
    • Organizer
      日本農芸化学会平成22年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] アンホテリシンBの抗真菌活性発現機構-液胞膜の断片化に液強を与える因子-2010

    • Author(s)
      荻田亮、藤田憲一、田中俊雄
    • Organizer
      日本生物工学会平成22年度大会
    • Place of Presentation
      宮崎ワールドコンベンションセンター
    • Year and Date
      2010-10-28
    • Related Report
      2010 Final Research Report
  • [Presentation] アンホテリシンBの抗真菌活性発現機構 -液胞膜の断片化に影響を与える因子-2010

    • Author(s)
      荻田亮、藤田憲一、田中俊雄
    • Organizer
      日本生物工学会平成22年度大会
    • Place of Presentation
      宮崎ワールドコンベンションセンター
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリミキシンBの抗真菌活性を増幅するタマネギ由来成分2010

    • Author(s)
      バヤラン 荻田亮、藤田憲一、土江松美、田中俊雄
    • Organizer
      日本農芸化学会平成21年度大会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 抗真菌化学療法の新たなターゲットの開拓-出芽酵母をモデルとして-2009

    • Author(s)
      荻田亮
    • Organizer
      関西応用微生物シンポジウム
    • Place of Presentation
      大阪市立大学
    • Year and Date
      2009-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 抗真菌化学療法の新たなターゲットの開拓 -出芽酵母をモデルとして-2009

    • Author(s)
      荻田亮
    • Organizer
      関西応用微生物シンポジウム
    • Place of Presentation
      大阪市立大学
    • Year and Date
      2009-12-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 病原性酵母Candida albicansに対するamphoterisin Bの液胞膜障害作用とアリシンによるその増幅2009

    • Author(s)
      ハスバガン、荻田亮、藤田憲一、平澤栄次、田中俊雄
    • Organizer
      日本農芸化学会平成20年度大会
    • Place of Presentation
      メッセマリン福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] A lemon-derived compound enhances and antivbacterial activity of salinomycin against Gram-negative bacteria2008

    • Author(s)
      荻田亮, 藤田憲一, 田中俊雄
    • Organizer
      国際バイオテクノロジーシンポジウム
    • Place of Presentation
      大連ワールドエキスポセンター
    • Year and Date
      2008-10-14
    • Related Report
      2010 Final Research Report
  • [Presentation] A lemon-derived compound enhances an antibacterial activity of salinomycin against Gram-negative bacteria2008

    • Author(s)
      Ogita, A., Fujita, K., Tanaka, T
    • Organizer
      International Biotechnology Symposium 2008
    • Place of Presentation
      大連ワールドエキスポ センター
    • Year and Date
      2008-10-14
    • Related Report
      2008 Annual Research Report
  • [Presentation] レモン果汁とサリノマイシンの組み合わせ2008

    • Author(s)
      荻田亮、藤田憲一、田中俊雄
    • Organizer
      日本生物工学会平成20年度大会
    • Place of Presentation
      東北学院大学
    • Year and Date
      2008-08-28
    • Related Report
      2010 Final Research Report
  • [Presentation] レモン果汁とサリノマイシンとの組み合わせによって生まれる新規の抗菌活性2008

    • Author(s)
      荻田 亮, 藤田 憲一, 田中俊雄
    • Organizer
      日本生物工学会 平成20年度大会
    • Place of Presentation
      東北学院大学
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 病原性酵母Candida albicansに対するamphotericin B の液胞膜障害作用とアリシンによるその増幅2008

    • Author(s)
      ハスバガン, 荻田 亮, 藤田 憲一, 平澤 栄次, 田中俊雄
    • Organizer
      日本農芸化学会 平成20年度大会
    • Place of Presentation
      メッセマリン福岡
    • Year and Date
      2008-03-28
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mchem/teacher.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mchem/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.sci.osaka-cu.ac.jp/biol/mchem/teacher.html

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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