| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Bacillus natto is a B. subtilis-related bacteria and produces sticky material, which is gamma polyglutamic acid (PGA). PGA is synthesized by PGA synthetase encoded by the pgsB gene. The regulation of pgsB involves the global-type transcription factor DegU. DegU is activated by phosphorylation. We determined the binding sites of DegU-P on the pgsB-control region. It was found that positive regulation of pgsB requires higher cellular concentration of DegU-P. Furthermore, ClpCP was indentified as a specific protease for DegU-P degradation. We confirmed that ClpCP degraded DegU-P in vitro.
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