Studies on regulation of poly-glutamic acid production by Bacillus subtilis transcription factor DegU.
| Project/Area Number |
20580084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokai University |
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Principal Investigator |
OGURA Mitsuo Tokai University, 海洋研究所, 教授 (80204163)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 枯草菌 / PGA / 転写制御 / 能動的タンパク質分解 / 2成分制御系 / ポリグルタミン酸 / ATP依存 / 発現制御 / 微生物 / 遺伝子 / DegU / シグナル伝達 |
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| Research Abstract |
Bacillus natto is a B. subtilis-related bacteria and produces sticky material, which is gamma polyglutamic acid (PGA). PGA is synthesized by PGA synthetase encoded by the pgsB gene. The regulation of pgsB involves the global-type transcription factor DegU. DegU is activated by phosphorylation. We determined the binding sites of DegU-P on the pgsB-control region. It was found that positive regulation of pgsB requires higher cellular concentration of DegU-P. Furthermore, ClpCP was indentified as a specific protease for DegU-P degradation. We confirmed that ClpCP degraded DegU-P in vitro.
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Report
(4 results)
Research Products
(18 results)
