Analysis of novel stress responses in Corynebacterium glutamicum

• Project/Area Number: 20580085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo Denki University
• Principal Investigator: NAKAMATSU Tsuyoshi Tokyo Denki University, 工学部, 教授 (40339065)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Hisashi 東京電機大学, 工学部, 教授 (90349788)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: コリネバクテリウム グルタミカム / Corynebacterium glutamicum / ストレス応答アミノ酸発酵 / コリネバクテリウム / ストレス応答 / アミノ酸発酵 / 機能未知遺伝子

Research Abstract

We analyzed 7 genes that are upregulated when glutamate overproduction is induced in Corynebacterium glutamicum. Our results suggested that 3 genes-NCgl2944, NCgl2945, and NCgl2946-were responsible for not only coping with the stress that induced the overproduction of glutamate but also growth in a synthetic medium. Further, we found significantly increased activities of 3 enzymes that catalyze reactions directly connected with the central carbon metabolism.

Research Products

• [Presentation] Corynebacterium glutamicumのNCgl2944-NCgl2946遺伝子破壊の影響 (2009)
  • Author(s): 鈴木康寛,橋本賢一,川崎寿,中松亘
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] Corynebacterium glutamicumのNCg12944〜NCgl2946遺伝子破壊の影響 (2009)
  • Author(s): 鈴木 康寛, 橋本 賢一, 川崎 寿, 中松 亘
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-28