Analysis of biological functions of the 7-methylguanosine modification in 16S rRNA
| Project/Area Number |
20580091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
OKSAMOTO Susumu National Agricultural Research Organization, 食品バイオテクノロジー研究領域, ユニット長 (80353986)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | RNA修飾 / 大腸菌 / 放線菌 / 二次代謝 / 16S rRNA / 7-メチルグアノシン |
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| Research Abstract |
Bacterial rRNA have many methylated nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. Here, we conducted genetic analyses of this conserved rRNA modification. Apparently, in Escherichia coli, the m7G modification is not essential for its growth under laboratory culture conditions. On the other hand, in Streptomyces coelicolor A3(2), this modification is critical for its viability under high temperature culture conditions. Furthermore, we found that the loss of the m7G modification in 16S rRNA often activates secondary metabolisms in the genus Streptomyces.
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Report
(4 results)
Research Products
(9 results)
