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Analysis of biological functions of the 7-methylguanosine modification in 16S rRNA

Research Project

Project/Area Number 20580091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNational Agricultural Research Organization

Principal Investigator

OKSAMOTO Susumu  National Agricultural Research Organization, 食品バイオテクノロジー研究領域, ユニット長 (80353986)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRNA修飾 / 大腸菌 / 放線菌 / 二次代謝 / 16S rRNA / 7-メチルグアノシン
Research Abstract

Bacterial rRNA have many methylated nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. Here, we conducted genetic analyses of this conserved rRNA modification. Apparently, in Escherichia coli, the m7G modification is not essential for its growth under laboratory culture conditions. On the other hand, in Streptomyces coelicolor A3(2), this modification is critical for its viability under high temperature culture conditions. Furthermore, we found that the loss of the m7G modification in 16S rRNA often activates secondary metabolisms in the genus Streptomyces.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (9 results)

All 2010 2009 2008

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] 薬剤耐性変異の導入による翻訳装置および転写酵素の改変と微生物機能の高度利用2010

    • Author(s)
      岡本晋
    • Journal Title

      食糧 48

      Pages: 63-77

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes2009

    • Author(s)
      田中幸徳、小松護、岡本晋、徳山真治、梶昭、池田治夫、越智幸三
    • Journal Title

      Applied and environmental microbiology Vol.75、No.14

      Pages: 4919-4922

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibiotic overproduction by rpsL and rsmG mutants of various streptomycetes2009

    • Author(s)
      田中幸徳
    • Journal Title

      Applied and Environmental Microbiology VOL 75

      Pages: 4919-4922

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 薬剤耐性変異の導入による微生物機能の活性化2010

    • Author(s)
      岡本晋
    • Organizer
      微生物資源ワークショップ
    • Place of Presentation
      玉川大学(東京都)
    • Year and Date
      2010-11-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] rsmG mutations-novel links between streptomycin resistance and antibiotic overproduction-2008

    • Author(s)
      岡本晋
    • Organizer
      UK-Japan Workshop 2008 Genomics of antibiotic producing actinomycetes
    • Place of Presentation
      日本大学ホール(東京)
    • Year and Date
      2008-10-31
    • Related Report
      2010 Final Research Report
  • [Presentation] rsmG mutations-novel links between streptomycin resistance and antibiotic production-2008

    • Author(s)
      岡本晋
    • Organizer
      UK-Japan Workshop 2008 Genomics of Antibiotic-producing Actinomycetes
    • Place of Presentation
      日本大学ホール(東京)
    • Year and Date
      2008-10-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2)2008

    • Author(s)
      岡本晋
    • Organizer
      第4回日本-フィンランドバイオテクノロジーシンポジウム
    • Place of Presentation
      ガトーキングダムサッポロ(札幌市)
    • Year and Date
      2008-10-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Mutations is rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3 (2)2008

    • Author(s)
      岡本晋
    • Organizer
      第4回 日本-フィンランド バイオテクノロジー シンポジウム
    • Place of Presentation
      ガトーキングダムサッポロ(札幌市)
    • Year and Date
      2008-10-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] ストレプトマイシン弱耐性変異rsmG導入による streptomyces 属の二次代謝能の変化2008

    • Author(s)
      田中幸徳
    • Organizer
      日本放線菌学会2008年度大会
    • Place of Presentation
      富士屋ホテル(山梨市)
    • Year and Date
      2008-07-10
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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