Analysis of biological functions of the 7-methylguanosine modification in 16S rRNA

• Project/Area Number: 20580091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: National Agricultural Research Organization
• Principal Investigator: OKSAMOTO Susumu National Agricultural Research Organization, 食品バイオテクノロジー研究領域, ユニット長 (80353986)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: RNA修飾 / 大腸菌 / 放線菌 / 二次代謝 / 16S rRNA / 7-メチルグアノシン

Research Abstract

Bacterial rRNA have many methylated nucleotides. Among them, a 7-methylguanosine (m7G) modification in 16S rRNA is highly conserved among eubacteria. Here, we conducted genetic analyses of this conserved rRNA modification. Apparently, in Escherichia coli, the m7G modification is not essential for its growth under laboratory culture conditions. On the other hand, in Streptomyces coelicolor A3(2), this modification is critical for its viability under high temperature culture conditions. Furthermore, we found that the loss of the m7G modification in 16S rRNA often activates secondary metabolisms in the genus Streptomyces.

Research Products

• [Journal Article] 薬剤耐性変異の導入による翻訳装置および転写酵素の改変と微生物機能の高度利用 (2010)
  • Author(s): 岡本晋
  • Journal Title: 食糧 48 Pages: 63-77
  • Peer Reviewed
• [Journal Article] Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes (2009)
  • Author(s): 田中幸徳、小松護、岡本晋、徳山真治、梶昭、池田治夫、越智幸三
  • Journal Title: Applied and environmental microbiology Vol.75、No.14 Pages: 4919-4922
  • Peer Reviewed
• [Journal Article] Antibiotic overproduction by rpsL and rsmG mutants of various streptomycetes (2009)
  • Author(s): 田中幸徳
  • Journal Title: Applied and Environmental Microbiology VOL 75 Pages: 4919-4922
  • Peer Reviewed
• [Presentation] 薬剤耐性変異の導入による微生物機能の活性化 (2010)
  • Author(s): 岡本晋
  • Organizer: 微生物資源ワークショップ
  • Place of Presentation: 玉川大学(東京都)
  • Year and Date: 2010-11-27
• [Presentation] rsmG mutations-novel links between streptomycin resistance and antibiotic overproduction- (2008)
  • Author(s): 岡本晋
  • Organizer: UK-Japan Workshop 2008 Genomics of antibiotic producing actinomycetes
  • Place of Presentation: 日本大学ホール(東京)
  • Year and Date: 2008-10-31
• [Presentation] rsmG mutations-novel links between streptomycin resistance and antibiotic production- (2008)
  • Author(s): 岡本晋
  • Organizer: UK-Japan Workshop 2008 Genomics of Antibiotic-producing Actinomycetes
  • Place of Presentation: 日本大学ホール(東京)
  • Year and Date: 2008-10-31
• [Presentation] Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2) (2008)
  • Author(s): 岡本晋
  • Organizer: 第4回日本-フィンランドバイオテクノロジーシンポジウム
  • Place of Presentation: ガトーキングダムサッポロ(札幌市)
  • Year and Date: 2008-10-02
• [Presentation] Mutations is rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3 (2) (2008)
  • Author(s): 岡本晋
  • Organizer: 第4回 日本-フィンランド バイオテクノロジー シンポジウム
  • Place of Presentation: ガトーキングダムサッポロ(札幌市)
  • Year and Date: 2008-10-02
• [Presentation] ストレプトマイシン弱耐性変異rsmG導入による streptomyces 属の二次代謝能の変化 (2008)
  • Author(s): 田中幸徳
  • Organizer: 日本放線菌学会2008年度大会
  • Place of Presentation: 富士屋ホテル(山梨市)
  • Year and Date: 2008-07-10