Purification and analysis of the highly stable hydrogenase as a cytochrome b complex form.

• Project/Area Number: 20580094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Ibaraki University
• Principal Investigator: NISHIHARA HIROFUMI Ibaraki University, 農学部, 准教授 (10260465)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ヒドロゲナーゼ / シトクロムb / 蛋白質精製 / 膜蛋白質 / 可溶化 / 水素酸化細菌 / Hydrogenovibrio marinus / Hydrogenovihrio marinus

Research Abstract

Hydrogenases have been anticipated for their potential use in hydrogen production and development of alternative hydrogen fuel cell catalyst to noble platinum. In this study, we succeeded in purification of the highly stable hydrogenase as a cytochrome b complex form. It will enable us to perform further crystallogenic and structural study of the enzyme to obtain useful information for hydrogenase stabilization and development of stable mimics.

Research Products

• [Journal Article] Purification and characterization of a highly thermostable, oxygen-resistant, respiratory [NiFe]-hydrogenase from a marine, aerobic hydrogen-oxidizing bacterium Hydrogenovibrio marinus. (2011)
  • Author(s): K.S.Yoon, K.Fukuda, K.Fujisawa, H.Nishihara
  • Journal Title: International Journal of Hydrogen Energy 36 Pages: 7081-7088
  • Peer Reviewed
• [Journal Article] Purification and characterization of a highly thermostable, oxygen resistant, respiratory[NiFe]-hydrogenase from a marine, aerobic hydrogen-oxidizing bacterium Hydrogenovibrio marinus (2011)
  • Author(s): K.S.Yoon, K.Fukuda, K.Fujisawa, H.Nishihara
  • Journal Title: International Journal of Hydrogen Energy Volume: 36(採択済) Pages: 7081-7088
  • Peer Reviewed
• [Presentation] Hydrogenovibrio marinus由来膜結合型ヒドロゲナーゼ-シトクロム b複合体の精製と解析 (2010)
  • Author(s): 西原宏史
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-29
• [Presentation] Hydrogenovibrio marinas由来膜結合型ヒドロゲナーゼーシトクロムb複合体の精製と解析 (2010)
  • Author(s): 西原宏史、篠崎文治、会沢勇志、尹基石
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-29
• [Presentation] 好気性水素酸化細菌の水素ガス代謝能の応用 (2009)
  • Author(s): 西原宏史, 尹基石
  • Organizer: 第61回日本生物工学会大会シンポジウム
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-25
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