The molecular mechanism underlying the co-regulation of plasma membrane and F-actin

• Project/Area Number: 20580096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: IHARA Sayoko 東京大学, 大学院・農学生命科学研究科, 助教 (80292788)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞応答 / 細胞骨格 / 細胞膜 / アクチン / アクチ

Research Abstract

Cells change their morphology under various physiological conditions. Co-regulation of plasma membrane and cytoskeleton is required for these changes, the mechanism of which is not fully understood. We addressed the issue by functional analysis of SWAP-70, which binds to both membrane and actin in a stimulus-dependent way. We found that SWAP-70 binds to integrin. 1 in a pull down assay, and the activation of integrin. 1 rescued SWAP-70-deficient phenotype, suggesting that SWAP-70 may function by activating integrin. 1 through their interaction.

Research Products

• [Journal Article] A mutant of SWAP-70. A phosphatidylinositoltrisphosphate binding protein, transforms mouse embryo fibroblasts, which is inhibited by sanguinarine (2010)
  • Author(s): Y. Fukui, S. Ihara
  • Journal Title: PLoS One Volume: 5 Pages: 14180-14180
• [Journal Article] A mutant of SWAP-70, a phosphatidylinositoltrisphosphate binding protein, transforms mouse embryo fibroblasts, which is inhibited by sanguinarine (2010)
  • Author(s): Fukui Y, et al
  • Journal Title: PLoS one Volume: 5
  • Peer Reviewed
• [Journal Article] Protein phosphatase type 1-interacting protein Ysw1 is involved in proper septin organization and prospore membrane formation during sporulation (2009)
  • Author(s): M. Ishihara, Y. Suda, I. Inoue, T. Tanaka, T. Takahashi, X. D. Gao, Y. Fukui, S. Ihara, A. M. Neiman, H. Tachikawa
  • Journal Title: Eukaryot Cell Volume: 8 Pages: 1027-1037
• [Journal Article] Protein phosphatase type 1-interacting protein Ysw1 is involved in proper septin organization and prospore membrane formation during sporulation (2009)
  • Author(s): Ishihara M, et al.
  • Journal Title: Eukaryotic Cell 8 Pages: 1027-37
  • Peer Reviewed
• [Presentation] 出芽酵母の前胞子膜伸長に必要なPP1複合体の機能解析 (2010)
  • Author(s): 大平孝博
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Presentation] Rac1結合タンパク質DEF6の機能解析 (2008)
  • Author(s): 加勢優子、高原有未、岡努、福井泰久、伊原さよ子
  • Organizer: 日本分子生物学会、日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Rap1 activation is required for DEF6-mediated cell shape change (2008)
  • Author(s): Yuko Kase, Tsutomu Oka, Yasuhisa Fukui, Sayoko Ihara
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-06-30