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Study on the interactions between animal lectins and carbohydrate chains based on their three-dimensional structures

Research Project

Project/Area Number 20580100
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionNagasaki University

Principal Investigator

HATAKEYAMA Tomomitsu  Nagasaki University, 工学部, 教授 (50228467)

Co-Investigator(Kenkyū-buntansha) UNNO Hideaki  長崎大学, 大学院・工学研究科, 助教 (10452872)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsレクチン / 糖鎖 / X線結晶構造解析 / 部位特異的変異 / 分子認識 / 糖結合タンパク質 / カルシウム / 糖認識機構 / 部位特異的変異体 / 蛋白質工学 / X線結晶解析
Research Abstract

Interactions between carbohydrate-binding proteins (lectins) and carbohydrate chains were analyzed based on their three-dimensional structures. The results demonstrated that that various carbohydrate chains can be recognized by combinations of amino acid residues located in the binding sites of lectins, which provides useful information to design novel molecular-recognition proteins. In addition, it was also revealed that binding of carbohydrates to lectin may induce conformational changes, which lead further biological activities to cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (55 results)

All 2011 2010 2009 2008 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (34 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPN carbohydrate-recognition motif2011

    • Author(s)
      Tomomitsu Hatakeyama, Takuro Kamiya, Masami Kusunoki, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Shuichiro Goda, Hideaki Unno
    • Journal Title

      Journal of Biological Chemistry 286(12)

      Pages: 10305-10315

    • NAID

      120006985160

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPNcarbohydrate-recognition motif2011

    • Author(s)
      Tomomitsu Hatakeyama, Takuro Kamiya, Masami Kusunoki, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Shuichiro Goda, Hideaki Unno
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286(12) Pages: 10305-10315

    • NAID

      120006985160

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitogenic activity of CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, isolated from the marine invertebrate Cucumaria echinata (Holothuroidea), Bioscience2010

    • Author(s)
      Zedong Jiang, Daekyung Kim, Yasuhiro Yamasaki, Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, Tatsuya Oda
    • Journal Title

      Biotechnology, and Biochemistry 74(8)

      Pages: 1613-1616

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitogenic activity of CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, isolated from the marine invertebrate Cucumaria echinata (Holothuroidea)2010

    • Author(s)
      Zedong Jiang, Daekyung Kim, Yasuhiro Yamasaki, Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, Tatsuya Oda
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74(8) Pages: 1613-1616

    • NAID

      10027558164

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CEL-I, an N-Acetylgalactosamine (GalNAc)-Specific C-Type Lectin, Induces nitric oxide production in RAW264.7 mouse macrophage cell line2009

    • Author(s)
      Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, Tatsuya Oda
    • Journal Title

      Journal of Biochemistry 146(2)

      Pages: 209-217

    • NAID

      10026302718

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Ca^<2+> on Refolding of the Recombinant Hemolytic Lectin CEL-III2009

    • Author(s)
      Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda, Tomomitsu Hatakeyama
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73(5)

      Pages: 1203-1205

    • NAID

      10027541543

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the valine clusters in domain 3 of the hemolytic lectin CEL-III in its oligomerization and hemolytic bilities2009

    • Author(s)
      Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda, Tomomitsu Hatakeyama
    • Journal Title

      Protein and Peptide Letters 16(4)

      Pages: 411-414

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the valine clusters in domain 3 of the hemolytic lectin CEL-III in its oligomerization and hemolytic abilities2009

    • Author(s)
      Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda, Tomomitsu Hatakeyama
    • Journal Title

      Protein and Peptide Letters 16(4)

      Pages: 411-414

    • NAID

      120006984476

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Ca^<2+>on refolding of the recombinant hemolytic lectin CEL-III2009

    • Author(s)
      Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda, Tomomitsu Hatakeyama
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73(5)

      Pages: 1203-1205

    • NAID

      10027541543

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CEL-I, an N-acetylgalactosamine(GaINAc)-specific C-type lectin, induces nitric oxide production in RAW264.7 mouse macrophage cell line2009

    • Author(s)
      Tomohiro Yamanishi, Tomomitsu Hatakeyama, Kenichi Yamaguchi, Tatsuya Oda
    • Journal Title

      Journal of Biochemistry 146(2)

      Pages: 209-217

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the α-helix region in domain 3 of the hemolytic lectin CEL-III : Implications for self-oligomerization and hemolytic processes2008

    • Author(s)
      Keigo Hisamatsu, Nobuaki Tsuda, Shuichiro Goda, Tomomitsu Hatakeyama
    • Journal Title

      Journal of Biochemistry 143(1)

      Pages: 79-86

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibacterial activity of short peptides based on pleurocidin and their interaction with phospholipid membranes2008

    • Author(s)
      Nobuaki Tsuda
    • Journal Title

      Bull. Chem. Soc Jpn. 81

      Pages: 1299-1303

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modifications of hydrophobic value and hydrophobic moment value of cationic model peptides for conversion of peptide-membrane interactions2008

    • Author(s)
      Junichi Taira
    • Journal Title

      Bull. Chem. Soc Jpn. 81

      Pages: 733-739

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the alpha-helix region in domain 3 of the hemolytic lectin CEL-III : Implications for self-oligomerization and hemolytic processes2008

    • Author(s)
      Keigo Hisamatsu
    • Journal Title

      J. Biochem. 143

      Pages: 79-86

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 海洋性棘皮動物グミ由来GalNAc特異的レクチン,CEL-Iのマイトジェニック活性2010

    • Author(s)
      Zedong Jiang, 畠山智充, 山口健一, 小田達也
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cucumaria echinata由来溶血性レクチンCEL-III膜孔形成複合体の結晶化2010

    • Author(s)
      海野英昭, 竪谷勇樹, 郷田秀一郎, 畠山智充
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] C型レクチンCEL-IVの糖結合部位EPN配列と糖鎖認識機構2010

    • Author(s)
      畠山智充, 神矢拓朗, 平林淳, 郷田秀一郎, 海野英昭
    • Organizer
      日本農芸化学会西日本支部大会
    • Place of Presentation
      熊本市
    • Year and Date
      2010-09-18
    • Related Report
      2010 Final Research Report
  • [Presentation] C型レクチンCEL-IVの糖結合部位EPN配列と糖鎖認識機構2010

    • Author(s)
      畠山智充, 神矢拓朗, 平林淳, 郷田秀一郎, 海野英昭
    • Organizer
      日本農芸化学会西日本支部大会
    • Place of Presentation
      崇城大学(熊本市)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海産無脊椎動物レクチンCEL-IIIの溶血活性発現に関与するアミノ酸残基の同定2010

    • Author(s)
      久松啓伍, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 溶血性レクチンの多量体化における構造変化のX線小角散乱による解析2010

    • Author(s)
      郷田秀一郎, 長尾知直, 貞方仁, 久松啓伍, 柊弓絃, 畠山智充
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 種々の糖鎖を付加したデンドリマーとレクチンとの凝集体形成反応2010

    • Author(s)
      狩野良太, 寺井康了, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2010-05-23
    • Related Report
      2010 Final Research Report
  • [Presentation] C型レクチンCEL-IVの部位特異的変異体と特異的糖との相互作用解析2010

    • Author(s)
      本多麻美, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2010-05-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 糖結合部位アミノ酸の置換によるC型レクチンCEL-Iの糖結合特異性の変換2010

    • Author(s)
      馬場智大, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2010-05-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 種々の糖鎖を付加したデンドリマーとレクチンとの凝集体形成反応2010

    • Author(s)
      狩野良太, 寺井康了, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] C型レクチンCEL-IVの部位特異的変異体と特異的糖との相互作用解析2010

    • Author(s)
      本多麻美, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 糖結合部位アミノ酸の置換によるC型レクチンCEL-Iの糖結合特異性の変換2010

    • Author(s)
      馬場智大, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超好熱アーキア Pyrococcus horikoshii由来PH0233産物の遺伝子組換え発現系の構築と可溶化条件の検討2010

    • Author(s)
      松原悠太, 久松啓伍, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] 超好熱アーキアSulfolobus tokodaii由来ST1670産物の遺伝子組換え発現系の構築と可溶化条件の検討2010

    • Author(s)
      池田光司, 福田泰信, 真方山慶佑, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] 超好熱アーキア由来糖結合タンパク質の探索2010

    • Author(s)
      栗浦良, 海野英昭, 畠山智充, 郷田秀一郎
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] 海洋性棘皮動物グミ由来GalNAc特異的レクチン,CEL-Iのマイトジェニック活性2010

    • Author(s)
      Zedong Jiang, 畠山智充, 山口健一, 小田達也
    • Organizer
      BMB2010(33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] Cucumaria echinata由来溶血性レクチンCEL-III膜孔形成複合体の結晶化2010

    • Author(s)
      海野英昭, 竪谷勇樹, 郷田秀一郎, 畠山智充
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] 溶血性レクチンの多量体化における構造変化のX線小角散乱による解析2010

    • Author(s)
      郷田秀一郎, 長尾知直, 貞方仁, 久松啓伍, 柊弓絃, 畠山智充
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Related Report
      2010 Final Research Report
  • [Presentation] 海産無脊椎動物レクチンCEL-IIIの溶血活性発現に関与するアミノ酸残基の同定2010

    • Author(s)
      久松啓伍, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Related Report
      2010 Final Research Report
  • [Presentation] 糖鎖付加ポリマーを用いたレクチン-糖鎖間相互作用解析2009

    • Author(s)
      畠山智充, 寺井康了, 田中亜由美, 海野英昭, 郷田秀一郎, 志波公平
    • Organizer
      日本農芸化学会西日本支部合同大会
    • Place of Presentation
      およびシンポジウム
    • Related Report
      2010 Final Research Report
  • [Presentation] 糖鎖含有デンドリマーと動的光散乱法を用いたレクチン-糖鎖間相互作用解析2009

    • Author(s)
      畠山智充, 寺井康了, 田中亜由美, 海野英昭, 郷田秀一郎, 志波公平
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸市
    • Related Report
      2010 Final Research Report
  • [Presentation] 光散乱法を用いたレクチン/糖含有ポリマー間相互作用の解析2009

    • Author(s)
      寺井康了, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      第33回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      唐津市
    • Related Report
      2010 Final Research Report
  • [Presentation] 海産無脊椎動物グミ由来溶血性レクチンCEL-IIIのX線小角散乱による多量体構造及び形成機構の解析2009

    • Author(s)
      郷田秀一郎, 貞方仁, 久松啓伍, 柊弓絃, 畠山智充
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本市
    • Related Report
      2010 Final Research Report
  • [Presentation] 溶血性レクチンCEL-IIIの自己会合ドメイン中のアミノ酸残基の役割2009

    • Author(s)
      久松啓伍, 海野英昭, 郷田秀一郎, 畠山智充(長崎大・工・応化)
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本市
    • Related Report
      2010 Final Research Report
  • [Presentation] 樹状細胞表面受容体BDCA-2のC型レクチンドメインの大腸菌による発現2009

    • Author(s)
      佐知望美, 林雅臣, 吉元みなみ, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡市
    • Related Report
      2010 Final Research Report
  • [Presentation] 海産無脊椎動物グミ由来溶血性レクチンCEL-III会合体のX線小角散乱による構造解析2009

    • Author(s)
      郷田秀一郎, 貞方仁, 久松啓伍, 柊弓絃, 畠山智充
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡市
    • Related Report
      2010 Final Research Report
  • [Presentation] 光散乱法によるレクチン-糖鎖間相互作用解析2008

    • Author(s)
      畠山智充, 田中亜由美, 貞方仁, 石丸絵理, 海野英昭, 郷田秀一郎, 志波公平
    • Organizer
      平成20年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 溶血性レクチンCEL-III会合ドメインの部位特異的変異体作製とその性質2008

    • Author(s)
      久松啓伍, 海野英昭, 郷田秀一郎, 畠山智充
    • Organizer
      平成21年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] グミ由来CEL-III膜貫通型複合体のX線結晶構造解析2008

    • Author(s)
      松本尚樹, 貞方仁, 郷田秀一郎, 海野英昭, 畠山智充
    • Organizer
      平成22年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光散乱法によるレクチン-糖鎖間相互作用解析2008

    • Author(s)
      畠山智充
    • Organizer
      平成20年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎大学文教キャンパス(長崎市)
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 溶血性レクチンCEL-III会合ドメインの部位特異的変異体作製とその性質2008

    • Author(s)
      久松啓伍
    • Organizer
      平成20年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎大学文教キャンパス(長崎市)
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] グミ由来CEL-III膜貫通型複合体のX線結晶構造解析2008

    • Author(s)
      松本尚樹
    • Organizer
      平成20年度日本農芸化学会西日本支部大会
    • Place of Presentation
      長崎大学文教キャンパス(長崎市)
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] α-ガラクトシド特異的C型レクチンCEL-IVと糖との相互作用解析2008

    • Author(s)
      畠山智充, 石井啓介, 神矢拓朗, 郷田秀一郎, 海野英昭
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-05-18
    • Related Report
      2010 Final Research Report
  • [Presentation] α-ガラクトシド特異的C型レクチンCEL-IVと糖との相互作用解析2008

    • Author(s)
      畠山智充
    • Organizer
      日本生化学会九州支部例会
    • Place of Presentation
      九州大学箱崎キャンパス(福岡市)
    • Year and Date
      2008-05-18
    • Related Report
      2008 Annual Research Report
  • [Book] はじめて学ぶ生命科学の基礎(畠山智充,小田達也編著)2010

    • Author(s)
      畠山智充
    • Total Pages
      195
    • Publisher
      化学同人
    • Related Report
      2010 Final Research Report
  • [Book] Hemolytic Lectin in Marine Invertebrates, Animal Lectins (G.R. Vast, H. Ahmed,eds.)2008

    • Author(s)
      畠山智充
    • Publisher
      CRC Press
    • Related Report
      2010 Final Research Report
  • [Book] Hemolytic Lectin in Marine Invertebrates, Animal Lectins(G. R. Vasta and H. Ahmed, eds. )2008

    • Author(s)
      Tomomitsu Hatakeyama
    • Total Pages
      558
    • Publisher
      CRC Press
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://naosite.lb.nagasaki-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ch.nagasaki-u.ac.jp/bio/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ch.nagasaki-u.ac.jp/bio/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ch.nagasaki-u.ac.jp/bio/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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