• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Recycling recovery of biomass and refinaly of the several useful compounds using the enzymatic coupling reaction system from earthworm and the enzymes from it's intesitinarl microorganisums.

Research Project

Project/Area Number 20580101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionOkayama Prefectural University

Principal Investigator

NAKAJIMA Nobuyoshi  Okayama Prefectural University, 保健福祉学部, 准教授 (10198070)

Co-Investigator(Renkei-kenkyūsha) ISHIHARA Kohji  岡山理科大学, 理学部, 准教授 (50273537)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsミミズ / プロテアーゼ / セルラーゼ / キシラナーゼ / 酸化還元酵素 / バイオマス / 放線菌 / 多糖類分解酵素
Research Abstract

The construction of the recycling-recovery system of several useful compounds from biomass with the use of the enzymatic coupling reactions was performed as a final study goal. At first, the proteases from earthworm and the enzymes (polysaccharide degrading enzymes and carbonyl reductases) from it's intestinal bacteria were selected and purified, and then these enzymological characterization was investigated for the elucidation of the structure and catalytic functions of these enzyme proteins.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (50 results)

All 2010 2009 2008

All Journal Article (21 results) (of which Peer Reviewed: 10 results) Presentation (24 results) Book (5 results)

  • [Journal Article] Biotransformation of Naringin and Naringenin by Cultured Eucalyptus perriniana Cells.2010

    • Author(s)
      K. Simoda, N. Kubota, K. Taniuchi, D. Sato, N.Nakajima, H. Hamada
    • Journal Title

      Phytochemistry 71

      Pages: 201-205

    • Related Report
      2010 Final Research Report
  • [Journal Article] Preparation of Chiral 2-Chloromandelamide : Stereoselective Reduction of an Aromatic alfa-Keto Amide with Actinomycete Strains.2010

    • Author(s)
      K.Ishihara, M. Nishimura, M. Nishi, N. Masuoka, N.Nakajima
    • Journal Title

      Biochem. Insights 3

      Pages: 19-24

    • Related Report
      2010 Final Research Report
  • [Journal Article] Isoquercitrin Improved the Health of Japanese Pearl Oyster, Pinctada fucata martensii, Affected by Akoya Oyster Disease.2010

    • Author(s)
      Y. Uchimura, K.Ishihara, N.Nakajima
    • Journal Title

      Aqua.Sci. 58(1)

      Pages: 151-153

    • NAID

      10029846035

    • Related Report
      2010 Final Research Report
  • [Journal Article] One-procedure Synthesis of Capsiate from Capsaicin by Lipase-catalyzed Dinamic Transacylation.2010

    • Author(s)
      K.Ishiahra, S-I. Kwon, N. Masuoka, N.Nakajima, H. Hamada
    • Journal Title

      W.J.Microbiol.Biotechnol 26

      Pages: 1337-1340

    • Related Report
      2010 Final Research Report
  • [Journal Article] Preparation of Arbutin Derivatives : Lipase-catalyzed Direct Acylation without the Need of Vinyl Ester as an Acyl Donor.2010

    • Author(s)
      K.Ishiahra, Y. Katsube, N. Kumazawa, N. Masuoka, N.Nakajima
    • Journal Title

      J.Biosci.Bioeng 109(6)

      Pages: 554-556

    • Related Report
      2010 Final Research Report
  • [Journal Article] Biotransformation of Cinnamic acid, p-Coumaric acid, Caffeic acid, and Ferulic acid by Plant Cultured Cells, Eucaryputus periniana.2010

    • Author(s)
      H. Katsuragi, K. Simoda, H. Hamada, N.Nakajima
    • Journal Title

      Biosci. Biotechnol. Biochem 74(9)

      Pages: 1920-1924

    • Related Report
      2010 Final Research Report
  • [Journal Article] Biotransformation of Cinnamic acid, p-Coumaric acid, Caffeic acid, and Ferulic acid by Plant Cultured Cells, Eucaryputus periniana.2010

    • Author(s)
      H.Katyuragi, N.Nakajima, et.al
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 1920-1924

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biotransformation of Naringin and Naringenin by Cultured Eucalyptusperriniana Cells2010

    • Author(s)
      K.Simoda, N.Nakajima, et.al
    • Journal Title

      Phytochemistry

      Volume: 71 Pages: 201-205

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Chiral 2-chloromandelamide : Stereoselective Reduction ofan Aromatic alfa-keto amide with Actinomycete Strains2010

    • Author(s)
      K.Ishihara, N.Nakajima, et.al
    • Journal Title

      Biochemistry Insights

      Volume: 3 Pages: 19-24

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isoquercitrin Improved the Health of Japanese Pearl Oyster, Pinctada fucata martensii, Affected by "Akoya Oyster Disease".2010

    • Author(s)
      U.Uchimura, N.Nakajima, et.al
    • Journal Title

      Aqua.Sci.

      Volume: 58 Pages: 151-153

    • NAID

      10029846035

    • Related Report
      2010 Annual Research Report
  • [Journal Article] One-procedure Synthesis of Capsiate from Capsaicin by Lipase-catalyzed Dinamic Transacylation.2010

    • Author(s)
      K.Ishihara, N.Nakajima, et.al
    • Journal Title

      W.J.Microbiol.Biotechnol.

      Volume: 26 Pages: 1337-1340

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biotransformation of Naringin and Naringenin by Cultured Eucalyptus perriniana Cells2010

    • Author(s)
      K.Simoda, N.Nakajima, et.al
    • Journal Title

      Phytochemistry 71

      Pages: 201-205

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Chiral 2-chloromandelamide : Stereoselective Reduction of an Aromatic alfa-keto amide with Actinomycete Strains2010

    • Author(s)
      K.Ishihara, N.Nakajima, et.al
    • Journal Title

      Biochemistry Insights 3

      Pages: 19-24

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of (2R,3S)-2-Benzamidometyhl-3-hydroxybutanoates by Immobilized Plant Cells of Parthenocissus tricuspidata.2009

    • Author(s)
      K. Simoda, N. Kubota, T. Kobayashi, N.Nakajima, H. Hamada
    • Journal Title

      Biochem. Insights 2

      Pages: 1-3

    • Related Report
      2010 Final Research Report
  • [Journal Article] 植物性素材を原料とした「乳酸菌・酵母発酵エキス」に関する研究2009

    • Author(s)
      桑木信輔、中島伸佳、石原浩二,双全、田中英彦
    • Journal Title

      微量栄養素研究 26

      Pages: 96-104

    • Related Report
      2010 Final Research Report
  • [Journal Article] Production of(2R, 3S)-2-Benzamido-methyl-3-hydroxybutanoates by Immobilized Plant Cells of Parthenocissus tricuspidata2009

    • Author(s)
      K.Simoda, N.Nakajima, et.al
    • Journal Title

      Biochemistry Insights 2

      Pages: 1-3

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of (2R, 3S)-2-Benzamidometyhl-3-hydroxybutanoates by Immobilized Plant Cells of Parthenocissus tricuspidata.2009

    • Author(s)
      K. Simoda, N, Kubota, N. Nakajima., H. Hamada
    • Journal Title

      Biochemistry Insights 2

      Pages: 1-3

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Unnatural Mono- and Oligosaccharides of Farnesol, Geraniol, and (S)-Perillyl Alcohol by Biocatalytic Glycosylations.2008

    • Author(s)
      K. Simoda, S. Sakamoto, N.Nakajima, H Hamada
    • Journal Title

      Chem.Lett 37(5)

      Pages: 556-557

    • NAID

      10021080720

    • Related Report
      2010 Final Research Report
  • [Journal Article] Stereoselective Reduction of Carbonyl Compounds with Actinomycete : Purification and Characterization of Three Keto ester Reductase from Streptomyces avermitilis.2008

    • Author(s)
      K.Ishihara, C. Kato, H. Yamaguchi, R. Iwai, H. Hamada, N. Masuoka, N.Nakajima
    • Journal Title

      Biosci. Biotechnol. Biochem 72(12)

      Pages: 3249-3257

    • Related Report
      2010 Final Research Report
  • [Journal Article] Synthesis of Unnatural Mono- and Oligosaccharides of Farnesol, Geraniol, and (S)-Perillyl Alcohol by Biocatalytic Glycosylations.2008

    • Author(s)
      K. Simoda, S. Sakamoto, N. Nakajima, H Hamada
    • Journal Title

      Biotechnol. J. 2

      Pages: 556-557

    • NAID

      10021080720

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereoselective Reduction of Carbonyl Compounds with Actinomycete : Purification and Characterization of Three α-Keto Ester Reductase from Streptomyces avermitilis.2008

    • Author(s)
      K. Ishihara, C. Kato, H. Yamaguchi, R. Iwai, H. Hamada, N. Masuoka, N. Nakajima
    • Journal Title

      Biosci, Biotechnol. Biochem. 72

      Pages: 3249-3257

    • NAID

      10027535299

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Purification and Characterization of a novel keto ester reductase from a green alga, Chlorella sorokiniana : comparison of enzymological properties with other microbial keto ester reductases2010

    • Author(s)
      K. Ishihara, M. Yoshida, N. Nakajima, et. al.
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawai, USA)
    • Related Report
      2010 Final Research Report
  • [Presentation] Purification and Characterization of three keto ester redutases from Streptpmyces averumitillis : an approach based on protein chemistry and bioimfomatics.2010

    • Author(s)
      K. Ishiahra, H. Yamaguchi, N. Nakajima, et. al.
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Hawai, USA)
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞を用いたカテキン類の変換2010

    • Author(s)
      浜田、中島、他2名
    • Organizer
      日本化学会第90回大会
    • Place of Presentation
      大阪市
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞を用いたフラバノン類の物質変換2010

    • Author(s)
      山本、中島、他2名
    • Organizer
      日本化学会第90回大会
    • Place of Presentation
      大阪市
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞を活用した高機能性素材の開発2010

    • Author(s)
      浜田、中島、他2名
    • Organizer
      日本植物組織培養学会第83回大会
    • Place of Presentation
      岡山市
    • Related Report
      2010 Final Research Report
  • [Presentation] ナノファイバーを活用した環式および非環式モノテルペン類の物質変換2010

    • Author(s)
      浜田、中島、他2名
    • Organizer
      第54回香料・テルペンおよび精油化学に関する討論会
    • Place of Presentation
      甲府市
    • Related Report
      2010 Final Research Report
  • [Presentation] Prification and characterization of three alfa-keto ester reductases from Streptomyces avermitilis : an approach based on protein chemistry and bioinfomatics.2010

    • Author(s)
      K.Ishihara, N.Nakajima, et.al
    • Organizer
      Pacifichem, 2010
    • Place of Presentation
      Hawai, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] The effective synthesis of lead compounds of the drug development and food supplement : glycosylation of flavonoids using plant cultured cells.2010

    • Author(s)
      H.Hamada, N.Nakajima, et.al
    • Organizer
      Pacifichem, 2010
    • Place of Presentation
      Hawai, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Purification and charactarization of a novel keto ester reductase from the green algae, Chrorella sonokiniana : comparoson of enzymological properties with other microbial keto ester reductases.2010

    • Author(s)
      K.Ishihara, N.Nakajima, et.al
    • Organizer
      Pacifichem, 2010
    • Place of Presentation
      Hawai, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 岡山県特産品ピオーネを利用した機能性食品素材の開発に関する研究2009

    • Author(s)
      石原・中島、他2名
    • Organizer
      第13回岡山リサーチパーク研究発表展示会
    • Place of Presentation
      岡山市
    • Related Report
      2010 Final Research Report
  • [Presentation] 乳酸菌・酵母による植物発酵エキスに関する研究2009

    • Author(s)
      桑木, 中島、他2名
    • Organizer
      2009年度日本農芸化学会大会
    • Place of Presentation
      福岡市
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞によるフラバノン類の変換2009

    • Author(s)
      浜田, 中島、他4名
    • Organizer
      第42回酸化反応討論会
    • Place of Presentation
      仙台市
    • Related Report
      2010 Final Research Report
  • [Presentation] CGTaseを活用したアルブチン多糖配糖体の調製2009

    • Author(s)
      大広, 中島、他3名
    • Organizer
      第13回生体触媒化学シンポジウム
    • Place of Presentation
      高松市
    • Related Report
      2010 Final Research Report
  • [Presentation] Glycosylation of Biological Active Compounds Using Plant Cultured Cells2009

    • Author(s)
      H. Hamada, K. Shimoda, N. Nakajima, et. al.
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Hawai, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Glycosylation of Biological Active Compounds Using Plant Cultured Cells2009

    • Author(s)
      H.Hamada, N.Nakajima, et.al
    • Organizer
      Plant Biology, 2009
    • Place of Presentation
      Hawai, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物培養細胞によるナリンゲニンの配糖化2008

    • Author(s)
      笠原・中島、他3名
    • Organizer
      第11回生体触媒化学シンポジウム
    • Related Report
      2010 Final Research Report
  • [Presentation] 岡山県特産品を利用した機能性食品素材の開発に関する基礎的研究2008

    • Author(s)
      石原, 中島、他3名
    • Organizer
      第12回岡山リサーチパーク研究発表・展示会
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞によるレスベラトロールの配糖化2008

    • Author(s)
      小林, 中島、他4名
    • Organizer
      日本化学会第88回大会
    • Related Report
      2010 Final Research Report
  • [Presentation] ミミズ由来のセリンプロテアーゼのリパーゼ反応について2008

    • Author(s)
      中島, 石原、他1名
    • Organizer
      日本農芸化学会中四国支部第21回講演会
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞によるモノテルペンの配糖化2008

    • Author(s)
      小林、中島、他3名
    • Organizer
      第26回日本植物細胞分子生物学会
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞による活性フェノールの配糖化2008

    • Author(s)
      浜田、中島、他5名
    • Organizer
      第50回天然有機化合物討論会
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物培養細胞によるテルペン類の配糖化2008

    • Author(s)
      浜田、中島、他3名
    • Organizer
      第52回香料・テルペンおよび精油化学に関する討論会
    • Related Report
      2010 Final Research Report
  • [Presentation] 緑藻由来のケトエステル還元酵素の精製と酵素化学特徴の解明2008

    • Author(s)
      岩井・中島、石原、他2名
    • Organizer
      第12回生体触媒化学シンポジウム
    • Related Report
      2010 Final Research Report
  • [Presentation] ミミズ由来のセリンプロテアーゼのリパーゼ反応について2008

    • Author(s)
      中島仲佳, 杉本 学, 石原浩二
    • Organizer
      日本農芸化学会中四国支部第21回講演会
    • Place of Presentation
      岡山市
    • Related Report
      2008 Annual Research Report
  • [Book] 栄養科学NEXTシリーズ「基礎化学」、辻、中村編著、第6章、第1節 食品中の有機化合物の構造、及び、第2節 食品中の有機化合物の機能を分担執筆2010

    • Author(s)
      中島
    • Publisher
      講談社サイエンテイフィク(発刊)
    • Related Report
      2010 Final Research Report
  • [Book] 栄養科学NEXTシリーズ「基礎有機化学」、高橋、辻編著、第3章、第2節 アミノ酸とタンパク質、及び、第4節 ビタミンを分担執筆2010

    • Author(s)
      中島
    • Publisher
      講談社サイエンテイフィク(発刊)
    • Related Report
      2010 Final Research Report
  • [Book] 基礎化学(辻、中村編著)(栄養科学NEXTシリーズ)2010

    • Author(s)
      中島, 他
    • Total Pages
      135
    • Publisher
      講談社
    • Related Report
      2010 Annual Research Report
  • [Book] 基礎有機化学(高橋、辻、編著)(栄養科学NEXTシリーズ)2010

    • Author(s)
      中島, 他
    • Total Pages
      148
    • Publisher
      講談社
    • Related Report
      2010 Annual Research Report
  • [Book] 栄養科学シリーズNEXT「基礎化学」、他2010

    • Author(s)
      辻英明, 中島伸佳, 他
    • Total Pages
      200
    • Publisher
      講談社サイエンテフィク
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi