Improve the specificity of a PrP^<Sc>-degrading enzyme and application for industry

• Project/Area Number: 20580106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyushu Sangyo University
• Principal Investigator: MITSUIKI Shinji 九州産業大学, 工学部, 教授 (70320140)
• Co-Investigator(Kenkyū-buntansha): GOTO Masatoshi 九州大学, 農学研究院, 寄付講座教員 (90274521) ; OKA Tatsuzo 鹿児島大学, 農学部, 教授 (50116795) ; KANOUCHI Hiroaki 鹿児島大学, 農学部, 准教授 (10351884)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 異常プリオン / ケラチナーゼ / プロテアーゼ / ケラチン

Research Abstract

The region concluding Arg 117 and 133 of NAPase had the keratin adsorbability, and was concerned with PrP^<Sc>-degrading activity. The specificity to hard-to-degrading protein of NAPase was improved by random mutagenesis. A specific inhibitor of NAPase adaptable for meet cleaner was discovered from bitter gourd.

Research Products

• [Journal Article] Decomposition of insoluble and hard-to-degrade animal proteins by enzyme E77 and its potentialapplications (2012)
  • Author(s): Zhao Hui, Shinji Mitsuiki, Mikako Takasugi, Masatoshi Goto, Hiroaki Kanouchi, and Tatsuzo Oka.
  • Volume: 166(7)
  • Pages: 1758-68
  • Peer Reviewed
• [Journal Article] Decomposition of Insoluble and Hard-to-Degrade Animal Proteins by Enzyme E77 and Its Potential Applications (2012)
  • Author(s): Hui Zhao, et.al
  • Journal Title: Appl Biochem Biotechnol Volume: 166(7) Pages: 1758-1768
  • Peer Reviewed
• [Journal Article] Identification of an alkaliphilic actinomycetes that produces a PrP^<Sc>-degrading enzyme (2010)
  • Author(s): Shinji Mitsuiki, Mikako Arai, Mami Kurose, Takeomi Take, Keisuke Kito, Yasushi Moriyama, Masatoshi Goto, Hiroaki Kanouchi, and Tatsuzo Oka
  • Volume: 60(2)
  • Pages: 349-353
  • Peer Reviewed
• [Journal Article] Identification of alkaliphilic actinomycetes that produces a PrPr^<Sc>-degrading enzyme (2010)
  • Author(s): 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Journal Title: Annals of Microbiology 60 Pages: 349-353
  • Peer Reviewed
• [Journal Article] Identification of an alkaliphilic actinomycetes that produces a PrP^<Sc>-degrading enzyme (2010)
  • Author(s): Shinji Mitsuiki, et.al
  • Journal Title: Annals of Microbiology Volume: Vol.60,No.2 Pages: 349-353
  • Peer Reviewed
• [Journal Article] Prion degrading enzyme and application for food safety (2008)
  • Author(s): Shinji Mitsuki, Masatoshi Goto, Masashi Sakai, Hiroaki Kanouchi, Kensuke Furukawa and Tatsuzo Oka.
  • Journal Title: Shinji Journal of Biotechnology Volume: Vol.1136/S
  • Peer Reviewed
• [Journal Article] Prion degrading enzyme and application for food safety (2008)
  • Author(s): 満生慎二, 岡部正明, 境正志, 森山康司, 叶内宏明, 岡達三
  • Journal Title: Journal of Biotechnology 1
  • Peer Reviewed
• [Journal Article] Prion degrading enzyme and application for food safety (2008)
  • Author(s): Shinji Mitsuiki, et al.
  • Journal Title: Journal of Biotechnology Vo. 1 136/S
  • Peer Reviewed
• [Presentation] Functional analysis of genetic variation in PrPSc degrading enzyme(NAPase): effects on structure and enzyme activity (2011)
  • Author(s): Shinya Miyasue, Shinji Mitsuiki, Masatoshi Goto, masashi Sakai.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo city
  • Year and Date: 2011-09-08
• [Presentation] Functional analysis of genetic variation in PrP^<Sc> degrading enzyme (NAPase) : effects on structure and enzyme activity (2011)
  • Author(s): Shinya Miyasue, et.al
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Screening for novel enzymes degrade insoluble and hard-to-degrade animal proteins (2010)
  • Author(s): Ayano Miyasako, Shinji Mitsuiki, Masashi Sakai.
  • Organizer: The 23rd International Symposium on Chemical Engineering
  • Place of Presentation: Fukuoka city
  • Year and Date: 2010-12-04
• [Presentation] Construction of an expression system of prion protein degradation enzyme in Escherichia coli (2010)
  • Author(s): Shinya Miyasue, Shinji Mitsuiki, Masashi Sakai.
  • Organizer: The 23rd International Symposium on Chemica Engineering
  • Place of Presentation: Fukuoka city
  • Year and Date: 2010-12-04
• [Presentation] Construction of an expression system of prion protein degradation enzyme in Escherichia coli (2010)
  • Author(s): Shinya Miyasue, et.al
  • Organizer: The 23^<rd> International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学(福岡市)
  • Year and Date: 2010-12-04
• [Presentation] Screening for nonel enzymes degrade insoluble and hard-to-degrade animal proteins (2010)
  • Author(s): Ayano Miyasako, et.al
  • Organizer: The 23^<rd> International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学(福岡市)
  • Year and Date: 2010-12-04
• [Presentation] Screening for nonel enzymes degrade insoluble and hard-to-degrade animal proteins (2010)
  • Author(s): 宮迫綾乃, 満生慎二, 境正志
  • Organizer: The 23^<rd> International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学
• [Presentation] Construction of an expression system of prion protein degradation enzyme in Escherichia coli (2010)
  • Author(s): 宮末慎弥, 満生慎二, 境正志
  • Organizer: The 23^<rd> International Symposium on Chemical Engineering
  • Place of Presentation: 九州産業大学
• [Presentation] 難分解性タンパク質分解酵素阻害剤の精製と特性 (2009)
  • Author(s): 森明日香, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 化学関連支部合同九州大会・外国人研究者交流国際シンポジウム大会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-07-11
• [Presentation] 難分解性タンパク質分解酵素阻害剤の精製と特性 (2009)
  • Author(s): 森明日香, 他
  • Organizer: 第46回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場(北九州市)
  • Year and Date: 2009-07-11
• [Presentation] 難分解性タンパク質分解酵素阻害剤の精製と特性 (2009)
  • Author(s): 森明日香, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 第46回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場
• [Presentation] プリオンと同様な性質を有する過塩素酸可溶性タンパク質 (2008)
  • Author(s): 叶内宏明, 陳田洋介, 岡達三, 満生慎二
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-09
• [Presentation] 難分解性タンパク質分解酵素阻害剤の精製と特性 (2008)
  • Author(s): 森明日香, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 日本生物工学会・九州支部大会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-12-06
• [Presentation] 難分解性タンパク質分解酵素の機能と応用 (2008)
  • Author(s): 青谷浩司, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 日本生物工学会・九州支部大会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-12-06
• [Presentation] 過塩素酸可溶性タンパク質の高次構造の多様性 (2008)
  • Author(s): 叶内宏明, 陳田洋介, 岡達三, 満生慎二
  • Organizer: 第31回日本分子生物学会年会第81回生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 難分解性タンパク質分解酵素阻害剤の精製と特性 (2008)
  • Author(s): 森明日香, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 第15回日本生物工学会・九州支部熊本大会
  • Place of Presentation: 崇城大学
• [Presentation] 難分解性タンパク質分解酵素の機能と応用 (2008)
  • Author(s): 青谷弘司, 満生慎二, 後藤正利, 叶内宏明, 岡達三
  • Organizer: 第15回日本生物工学会・九州支部熊本大会
  • Place of Presentation: 崇城大学
• [Presentation] 難分解性タンパク質分解酵素生産微生物の同定と酵素の発現 (2008)
  • Author(s): 森明日香, 他
  • Organizer: 第15回日本生物工学会・九州支部熊本大会
  • Place of Presentation: 崇城大学
• [Remarks] ホームページ
  • URL: http://www.ip.kyusan-u.ac.jp/J/kougaku/tc/mitsuiki/index.html