Evolutionary molecular engineering and structure-activity relationship in cellulase condensation reaction.
| Project/Area Number |
20580107
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Ichinoseki National College of Technology |
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Principal Investigator |
TOTANI Kazuhide 一関工業高等専門学校, 物質化学工学科, 教授 (40369913)
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| Co-Investigator(Renkei-kenkyūsha) |
OKADA Hirofumi 長岡技術科学大学, 工学部・生物系, 准教授 (70233343)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 酵素利用学 / 糖鎖工学 / セルラーゼ / Trichoderma reesei / EG I / BGL / セロビオース / セロオリゴ糖 / 縮合 / 糖転移 / EGI / ラクトース / 進化分子工学 / N-アセチルラクトサミン |
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| Research Abstract |
Endoglucanase I(EG I) from filamentous fungus Trichoderma reesei has not been considered to hydrolyze the"cellobiose". On the other hand, the physiological role of a secretedβ-glucosidase I(BGL I) is not clear, which can hydrolyze cellobiose outside fungus body. T. reesei derived EG I, of which catalytic domain was used, generated glucose(product) from cellobiose(substrate) in a substrate concentration-dependent manner, and glucose production ratio reached to more than half of BGL I under the high substrate concentration such as 1 M. When T. reesei derived BGL I reacted with a high concentration of cellobiose, a remarkable synthesis of trisaccharide considered as transglycosylational product was observed. In the case that BGL I reacts with high concentrations of glucose, no condensational product was observed. The physiological role of BGL I is considered to be different to BGL II in the fungus body, which has been reported to generate the strongest cellulase inducer sophorose by the in vitro condensation of high concentration of glucose.
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Report
(4 results)
Research Products
(42 results)
