Chemical Biology of lifespan-extending substrates secreted by the nematode Caenorhabditis elegans

• Project/Area Number: 20580112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tottori University
• Principal Investigator: KAWANO Tsuyoshi Tottori University, 農学部, 教授 (50270567)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 寿命 / 休眠 / インスリン / 線虫 / 発現制御 / 情報分子 / ペプチドホルモン / 寿命制御 / シグナル伝達

Research Abstract

I have identified Caenorhabditis elegans insulin-like genes ins-6, ins-12, and ins-35. Expression of these genes was regulated by lifespan-extending substances secreted by C.elegans. Gene-disruption revealed that ins-6 and ins-12 were relevant to regulation of lifespan. In contrast, ins-35 was largely relevant to regulation of larval diapause not adult lifespan. In addition, analyses of expression patterns of ins-35 allowed me to understand the physiological function of the gene.

Research Products

• [Journal Article] An altered method of feeding RNAi that knocks down multiple genes simultaneously in the nametode Caenorhabditis elegans. (2010)
  • Author(s): Gouda K, Matsunaga Y, Iwasaki T, an Kawano T
  • Journal Title: Biosci.Biotechnol.Biochem. 74 Pages: 2361-2365
  • Peer Reviewed
• [Journal Article] Physiological function of INS-12, one of the Type-γ insulin-like peptides (2010)
  • Author(s): Matsunaga Y, Ito H, Kawano T
  • Journal Title: in C.elegans.Peptide Science Pages: 459-462
  • Peer Reviewed
• [Journal Article] An altered method of feeding RNAi that knocks down multiple genes simultaneously in the nametode Caenorhabditis elegans (2010)
  • Author(s): Gouda K, Matsunaga Y, Iwasaki T, Kawano T
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Pages: 2361-2365
  • Peer Reviewed
• [Journal Article] Physiological function of INS-12, one of the Type-g insulin-like peptides, in C. elegans (2010)
  • Author(s): Matsunaga, Y., Ito, H., Kawano, T.
  • Journal Title: Peptide Science Pages: 459-462
  • Peer Reviewed
• [Journal Article] Physiological function of type-β insulin-like genes (2008)
  • Author(s): Yonezawa Y, Kimura Y, Kawano T
  • Journal Title: in C.elegans.Peptide Science Pages: 525-528
  • Peer Reviewed
• [Journal Article] Physiological function of type-β・insulin-like genes in C. elegans (2008)
  • Author(s): Yonezawa, Y., Kimura, Y., Kawano, Y
  • Journal Title: Peptide Science Pages: 525-528
  • NAID: 10021906926
  • Peer Reviewed
• [Journal Article] Nematicidal activity of actylene compounds from Co repsis lanceolara L. (2008)
  • Author(s): Kimura, Y., Kawano, T., Fujioka, S., Shimada, A.
  • Journal Title: Z. Naturforsch[C] 6 Pages: 843-847
  • Peer Reviewed
• [Presentation] 線虫C.elegansの幼虫休眠を制御するインスリン様ペプチドINS-35 (2011)
  • Author(s): 片山侑希、阪本晃市、河野佳世、松永洋平、岩崎崇、河野強
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 線虫C.elegansのインスリン様ペプチドINS-18の発現制御機構 (2011)
  • Author(s): 松永洋平、玄行・安藤恵子、三谷昌平、岩崎崇、河野強
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 線虫C.elegansの幼虫休眠を制御するインスリン様ペプチドINS-35 (2011)
  • Author(s): 片山侑希、阪本晃市、河野佳世、松永洋平、岩崎崇、河野強
  • Organizer: 日本農芸化学会2011年度大学
  • Place of Presentation: 京都女子大(京都)
  • Year and Date: 2011-03-28
• [Presentation] 線虫C.elegansのインスリン様ペプチドINS-18の発現制御機構 (2011)
  • Author(s): 松永洋平、玄行・安藤恵子、三谷昌平、岩崎崇、河野強
  • Organizer: 日本農芸化学会2011年度大学
  • Place of Presentation: 京都女子大(京都)
  • Year and Date: 2011-03-27
• [Presentation] An approach to elucidate crosstalk between TGF-b and insulin/IGF-I signaling in Caenorhabditis elegans. (2010)
  • Author(s): 松永洋平, 安藤-玄行恵子, 三谷昌平, 岩崎崇, 河野佳世
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-06-13
• [Presentation] Analysis of a new pathway regulating larval diapuase in Caenorhabditis elegans. (2010)
  • Author(s): Yukari Omichi, Kenji Gouda, Takashi Iwasaki, Tsuyoshi Kawano
  • Organizer: 4th East Asia C.elegans Meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-06-12
• [Presentation] Analysis of a new pathway regulating larval diapause in Caenorhabditis elegans (2010)
  • Author(s): Omichi Y, Gouda K, Iwasaki T, Kawano T
  • Organizer: 4^<th> East Asia C.elegans Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2010-06-12
• [Presentation] An approach to elucidate crosstalk between TGF-β and insulin/IGF-1 signaling in Caenorhabditis elegans (2010)
  • Author(s): Matsunaga Y, Gengyo-Ando K, Mitani S, Iwasaki T, Kawano T
  • Organizer: 4^<th> East Asia C.elegans Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2010-06-12
• [Presentation] 線虫C.elegansのtype-γインスリン様ペプチドであるINS-12の生理学的機能 (2009)
  • Author(s): 松永洋平、伊藤弘子、河野強
  • Organizer: 第46回ペプチド討論会
  • Place of Presentation: 北九州
  • Year and Date: 2009-11-05
• [Presentation] Expression mechanism of INS-18, one of the insulin-like peptides, in C.elegans (2009)
  • Author(s): Yohei Matsunaga, Keiko Gengyo-Ando, Shohei Mitani, Tsuyosi Kawano
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: University of California, Los Angeles, USA
  • Year and Date: 2009-06-27
• [Presentation] Expression mechanism of INS-18, one of the insulin-like peptides, in C.elegans. (2009)
  • Author(s): Yohei Matsunaga, KeikoGengyo-Ando, Shohei Mitani, Tsuyoshi Kawano
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2009-06-26
• [Presentation] 線虫におけるインスリン様遺伝子の機能解析 (2009)
  • Author(s): 松永洋平, 伊藤弘子, 河野 強
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 線虫の休眠・寿命を制御するインスリン様ペプチド (2009)
  • Author(s): 松永洋平、安藤-玄行恵子、三谷昌平、河野強
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 線虫C.elegansにおける簡便な三重遺伝子機能抑制法 (2008)
  • Author(s): 合田健司、河野強
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 線虫の休眠・寿命を制御するインスリン様ペプチドINS-18の発現調節機構 (2008)
  • Author(s): 松永洋平、安藤-玄行恵子、三谷昌平、河野強
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] 線虫C. elegansのインスリン様分子INS-18の発現制御機構 (2008)
  • Author(s): 松永洋平, 安藤恵子, 三谷昌平, 河野 強
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Remarks] ホームページ等
  • URL: http://staff.muses.tottori-u.acjp/kawano/
• [Remarks]
  • URL: http://staff.muses.tottori-u.ac.jp/kawano/
• [Patent(Industrial Property Rights)] ネコブセンチュウpos-1遺伝子、及びその遺伝子発現制御によるネコブセンチュウ防除方法 (2009)
  • Inventor(s): 河野強
  • Industrial Property Rights Holder: 河野強
  • Filing Date: 2009-10-13
• [Patent(Industrial Property Rights)] ネコブセンチュウpos-1遺伝子、及びその遺伝子発現制御によるネコブセンチュウ防除方法 (2009)
  • Inventor(s): 河野強
  • Industrial Property Rights Holder: 鳥取大学
  • Industrial Property Number: 2009-236075
  • Filing Date: 2009-10-13