| Project/Area Number |
20580113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
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| Research Institution | Osaka City University |
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Principal Investigator |
FUJITA Ken-Ichi Osaka City University, 大学院・理学研究科, 准教授 (10285281)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | trans-anethole / PDR5遺伝子 / Candida albicans / fluconazole / 相乗的抗真菌作用 / 薬剤耐性 / 薬剤排出ポンプ / 出芽酵母 / アネトール / PDR5 / 相乗効果 / 抗真菌性抗生物質 / anethole / mitochondria / membrane potential / adenine nucleotide translocator / alpha-tocopherol / Saccharomyces cerevisiae / mitochondrial electron transport / reactive oxygen species |
|---|
| Research Abstract |
Dodecanol exhibits temporary fungicidal activity against budding yeast. In this study, we investigated expression mechanism for the durable fungicidal activity of a mitochondrion-disturbing agent, anethole, combined with dodecanol. In dodecanol-treated cells of S. cerevisiae, increase in the gene expression of PDR5 among ABC transporters was detected. On the other hand, in cells treated with dodecanol and anethole, over-expression of PDR5 gene was restricted. These results indicated anethole expressed durable fungicidal activity via restriction of PDR5 gene expression.
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