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Analysis of mycotoxin producibility by rice adherent Fusarium fungi

Research Project

Project/Area Number 20580116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionNational Agricultural Research Organization

Principal Investigator

KUSHIRO Masayo  National Agricultural Research Organization, 主任研究員 (40353932)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糸状菌 / イネ / フザリウム / マイコトキシン
Research Abstract

A full-length clone of putative polyketide synthase responsible for unknown mycotoxin biosynthesis was isolated from Fusarium graminearum, one of the rice adherent fungi. This clone was served for a heterologous expression in a transformed Aspergillus oryzae, which suggested this clone to be a novel polyketide synthase. Also, in vitro cultures of various Fusarium fungi followed by chromatographic analysis revealed the production of fumonisins by the fungus that had not been reported as a fumonisin-producer.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009 2008 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Preparation of an in-house reference material containing fumonisins in Thai rice and matrix extension of the analytical method for Japanese rice2010

    • Author(s)
      Norhafniza Awaludin, Reiko Nagata, Tomomi Kawasaki, Masayo Kushiro(責任著者)
    • Journal Title

      Toxins 1(2)

      Pages: 188-195

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Limited surveillance of fumonisins in brown rice and wheat harvested in Japan2009

    • Author(s)
      Masayo Kushiro(筆頭著者), Yazhi Zheng, Reiko Nagata, Hiroyuki Nakagawa, Hitoshi Nagashima
    • Journal Title

      Journal of Food Protection 72(6)

      Pages: 1327-1331

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Limited surveillance of fumonisins in brown rice and wheat harvested in Japan2009

    • Author(s)
      久城真代(筆頭著者), 他4名
    • Journal Title

      Journal of Food Protection Vol.72(6)

      Pages: 1327-1331

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of an in-house reference material containing fumonisins in Thai rice and matrix extension of the analytical method for Japanese rice2009

    • Author(s)
      久城真代(責任著者), 他3名
    • Journal Title

      Toxins Vol.1(2)

      Pages: 188-195

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Fusarium fujikuroiのフモニシン産生遺伝子クラスターの構造解明2011

    • Author(s)
      北嶋美葉、スコット暁子、久城真代、中島隆、景山幸二、百町満朗、須賀晴久
    • Organizer
      日本マイコトキシン学会第69回学術講演会
    • Place of Presentation
      タワーホール船堀,東京都江戸川区
    • Year and Date
      2011-01-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of possible fumonisin contamination in rice2010

    • Author(s)
      Masayo Kushiro, Norhafniza Awaludin, Hiroyuki Nakagawa, Hitoshi Nagashima
    • Organizer
      International Mycotoxin Conference 2010
    • Place of Presentation
      Park Royal Hotel, Penang, Malaysia
    • Year and Date
      2010-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of possible fumonisin contamination in rice2010

    • Author(s)
      久城真代
    • Organizer
      International Mycotoxin Conference 2010
    • Place of Presentation
      Penang, Malaysia
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] インハウスかび毒分析用コメ標準物質の作製とコメ中フモニシン分析法の開発2010

    • Author(s)
      久城真代
    • Organizer
      食総研・産総研ジョイントシンポジウム2010(EBIS303)
    • Place of Presentation
      東京都渋谷区
    • Year and Date
      2010-07-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Surveillance of fumonisins in brown rice and wheat harvested in Japan2009

    • Author(s)
      Masayo Kushiro, Hiroyuki Nakagawa, Hitoshi Nagashima
    • Organizer
      The 123^<rd> AOAC International Annual Meeting and Exposition
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2009-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 国産稲モミでのフモニシン検出2009

    • Author(s)
      久城真代、永田礼子、中川博之、長嶋, 等
    • Organizer
      第7回フザリウム研究会
    • Place of Presentation
      岐阜県長良川温泉旅館多賀
    • Year and Date
      2009-08-27
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] イネ着生フザリウム属糸状菌におけるフモニシン産生性の解析2008

    • Author(s)
      久城真代、川崎友美、中川博之、長嶋, 等
    • Organizer
      日本マイコトキシン学会第64回学術講演会
    • Place of Presentation
      名古屋市立大学薬学部
    • Year and Date
      2008-08-29
    • Related Report
      2010 Final Research Report
  • [Presentation] イネ着生フザリウム属糸状菌におけるフモニシン産生性の解析2008

    • Author(s)
      久城真代
    • Organizer
      日本マイコトキシン学会第64回学術講演会
    • Place of Presentation
      名古屋市立大学薬学部薬友会館
    • Year and Date
      2008-08-29
    • Related Report
      2008 Annual Research Report
  • [Book] 微生物胞子-制御と対策-(第6章第4節カビ毒p155-161)2011

    • Author(s)
      小西良子、久城真代
    • Total Pages
      370
    • Publisher
      サイエンスフォーラム
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.nfri.affrc.go.jp/guidance/soshiki/anzen/kagaku.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www/nfri.affrc.go.jp/guidance/soshiki/anzen/kagaku.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] 2009年成果は未入力

    • URL

      http://www.nfri.affrc.go.jp/guidance/soshiki/anzen/kagaku.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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