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Elucidation of genetic mechanisms that generate structural diversity of a group of sesquiterpenes involved in plant-microbe interactions

Research Project

Project/Area Number 20580117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KIMURA Makoto  独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (20261167)

Research Collaborator 富永 直樹  東京理科大学, 理工学部, 修士
前田 一行  明治大学, 農学部・農学科, 博士
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsBiosynthesis / Deacetylase / Fusarium graminearum / Nivalenol / Trichothecenes / マイコトキシン / 脱アセチル化酵素 / ムギ類赤かび病菌 / 二次代謝 / 遺伝子クラスター / 生合成 / セスキテルペン / 多機能酵素 / アセチル化酵素
Research Abstract

Fusarium graminearum, a head blight fungus that causes devastating disease of wheat and other important cereal crops, produces trichothecene mycotoxins, a diverse group of sesquiterpene secondary metabolites. They are also known to act as a virulence factor of this pathogen. In an attempt to elucidate the mechanisms that generate the diversity of side chains, we have identified and characterized a C-4 deaetylase gene, Tri104, outside of the gene trichothecene cluster. Tri104 was expressed when the fungus was grown on solid rice(SR), but not on liquid rice flour(RF) medium. This result coincides with the fact that the fungus produces nivalenol, a trichothecene with a hydroxyl at C-4, only on SR(but not on RF) medium.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] 4-O-aceylation and 3-O-acetylation of trichothecenes by tricho thecene 15-O-acetyltransferase encoded by Fusarium Tri32008

    • Author(s)
      東海武史
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 72

      Pages: 2485-2489

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An easy method to identify 8-keto-15-hydroxytrichothecenesby thin-layer chromatography2008

    • Author(s)
      安藤直子
    • Journal Title

      Mycotoxins 58

      Pages: 115-117

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニバレノール生産菌はフザレノンXのC-4位を脱アセチル化する酵素を有する2011

    • Author(s)
      富永直樹、小関徳文、伊澤真澄、鎌倉高志、吉田稔、木村真
    • Organizer
      日本マイコトキシン学会第67回学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 糸状菌二次代謝を昂進する因子の探索2010

    • Author(s)
      木村真、伊澤真澄、広瀬智哉、中村健道、大里修一、鎌倉高志、吉田稔
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ニバレノール生産菌はフザレノンXのC-4位を脱アセチル化する酵素を有する2010

    • Author(s)
      富永直樹、小関徳文、伊澤真澄、鎌倉高志、吉田稔、木村真
    • Organizer
      日本マイコトキシン学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-01-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] ニバレノール後期生合成経路の解析2010

    • Author(s)
      小関徳文、兵藤壮一郎、富永直樹、伊澤真澄、鎌倉高志、吉田稔、木村真
    • Organizer
      日本マイコトキシン学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-01-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] ムギ類赤かび病菌によるトリコテセン系かび毒産生機構の解析2009

    • Author(s)
      木村真
    • Organizer
      日本農芸化学会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2020-03-05  

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