| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Formation of alkyl radicals of unsaturated lipids is the first step of lipid peroxidation. We have studied the lipid-alkyl radical scavenging reactions of vitamin E (α-tocopherol, α-TH). When cholesteryl linoleate (ChL) hydroperoxides were reacted with α-TH at 100℃, two addition products were obtained as 6-O-epoxyChL-α-TH and 6-O-ChL-α-TH. Iron-catalyzed reaction of phosphatidylcholine (PC) hydroperoxides with α-TH under nitrogen gave the addition products, 6-O-epoxyPC-α-TH. A sensitive method using a high-performance liquid chromatography with post-column reduction using platinum as the catalyst followed by fluorescence detection has been developed for the quantification of the addition products of α-TH during the peroxidation of an emulsion of ChL and PC. The addition products of α-TH with alkyl and peroxyl radicals were detected when the emulsion was peroxidized. The results indicate that α-TH may react with lipid-alkyl radicals under oxygen-insufficient conditions.
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