• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the types of the taste cell and taste perception.

Research Project

Project/Area Number 20580128
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

HAYASHI Yukako  Kyoto University, 大学院・農学研究科, 准教授 (60212156)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords味覚 / マウス / 情報変換 / 苦味 / 味細胞 / 情報伝達 / カフェイン / 免疫染色 / カルシウム動員 / カルシウムチャネル / カルシウムリリース / ガストジューシン
Research Abstract

There are a lot of unsolved issues about the pathway from taste cells to taste cells or taste nerves. Therefore, we analyzed the caffeine transduction mechanism of mouse using behavioral test, calcium imaging, and immunocytochemistry. The responsiveness of taste cells to caffeine, ATP, and high concentration of K^+ were examined by calcium imaging followed by immunocytochemistry to detect expression of G_α-gustducin and IP_3R3 which are known as type II cell markers. As a result, caffeine stimulated taste cells both type II and the other type of cells. A part of type II cells was depolarized by high concentration of K^+, and taste cells which responded to caffeine and high K^+ existed. Therefore we concluded there are voltage-gated calcium channels in subset of type II cells. Also, ATP stimulates both type II and other type of cells. Considering the report that ATP is released from type II cells, it seems to play an autocrine role in taste cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (15 results)

All 2011 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Evaluation of Umami Substances and the Characterizationof Umami Receptor and Coupling G Protein.2011

    • Author(s)
      Masataka Narukawa, Yukako Hayashi
    • Journal Title

      Flavour and Fragrance Journal in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 味細胞タイプ別のカフェイン味刺激伝達経路の解析2010

    • Author(s)
      北田亮、藤戸洋聡、呉性姫、林由佳子
    • Journal Title

      日本味と匂い学会誌

      Volume: 3 Pages: 237-240

    • NAID

      110008451324

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Signalling mechanisms in mouse bitter responsive taste cells.2009

    • Author(s)
      Narukawa M, Minamisawa E, Hayashi Y.
    • Journal Title

      Neuroreport 20(10)

      Pages: 936-40

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 味細胞におけるカフェイン味刺激伝達経路の解析2009

    • Author(s)
      藤戸洋聡、谷口友朗、北田亮、呉性姫、松村康生、林由佳子
    • Journal Title

      日本味と匂い学会誌 16(3)

      Pages: 331-334

    • NAID

      110007568729

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Signalling mechanisms in mouse bitter responsive taste cells.2009

    • Author(s)
      Narukawa M, Minamisawa E, Hayashi Y.
    • Journal Title

      Neuroreport 20

      Pages: 936-940

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of umami receptor and coupling G protein in mouse taste cells.2008

    • Author(s)
      Narukawa M, Kitagawa-Iseki K, Oike H, Abe K, Mori T, Hayashi Y.
    • Journal Title

      Neuroreport 19

      Pages: 1169-73

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] L-theanine elicits an umami taste with inosine 5'-monophosphate.2008

    • Author(s)
      Narukawa M, Morita K, Hayashi Y.
    • Journal Title

      Biosci Biotechnol Biochem 72

      Pages: 3015-7

    • NAID

      10027534323

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 味細胞タイプ別のカフェイン味刺激伝達経路の解析2010

    • Author(s)
      北田亮、藤戸洋聡、呉性姫、林由佳子
    • Organizer
      日本味と匂学会
    • Place of Presentation
      福岡県北九州市
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evaluation of Umami Substances and the Characterization of Umami Receptor and Coupling G Protein.2010

    • Author(s)
      林由佳子
    • Organizer
      Taste and food acceptance : chemistry and molecular biology of sweet, bitter and umami taste
    • Place of Presentation
      Protida, Italy
    • Year and Date
      2010-04-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Evaluation of Umami substance and the characterization of umami receptor and coupling G protein2010

    • Author(s)
      林由佳子
    • Organizer
      Taste and food acceptance : chemistry and molecular biology of sweet, bitter and umami taste
    • Place of Presentation
      イタリアプロチーダ市(招待講演)
    • Year and Date
      2010-04-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 味細胞におけるカフェイン味刺激伝達経路の解析2009

    • Author(s)
      藤戸洋聡、谷口友朗、北田亮、呉性姫、松村康生、林由佳子
    • Organizer
      日本味と匂学会
    • Place of Presentation
      旭川市・旭川市民文化会館
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] PHYSIOLOGICAL CHARACTERIZATION OF UMAMI RECEPTOR AND THE G PROTEIN IN MOUSE TASTE CELLS2008

    • Author(s)
      Yukako Hayashi, Masataka Narukawa, Keiko Iseki, Tomohiko Mori
    • Organizer
      International Symposium on Olfaction and Taste
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2008-07-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] 味細胞タイプ別のカフェイン味刺激伝達経路の解析

    • Author(s)
      北田亮(林由佳子)
    • Organizer
      日本農芸化学2011年大会(地震のため口頭発表中止)
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 苦味を制御する鶏卵抗体の作製とその評価

    • Author(s)
      佐藤創平(林由佳子)
    • Organizer
      日本農芸化学2011年大会(地震のため口頭発表中止)
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 苦味受容体の抗体作成2010

    • Inventor(s)
      林由佳子、(株)ァーマシューズ、日本農業大学
    • Industrial Property Rights Holder
      林由佳子、(株)ァーマシューズ、日本農業大学
    • Filing Date
      2010-09-21
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi