Construction of evaluation system of metabolism for dietary compounds using human xenobiotic enzymes
| Project/Area Number |
20580138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
IKUSHIRO Shinichi Toyama Prefectural University, 工学部, 准教授 (50244679)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKI Toshiyuki 富山県立大, 工学部, 教授 (70293909)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 食品生化学 / 食品代謝物 / フラボノイド / グルクロン酸転移酵素 / シトクロムP450 / 食品 / バイオテクノロジー / 異物代謝 |
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| Research Abstract |
Dietary compounds such as polyphenols are conjugated by UGT and SULT isoforms and its biological effects depend, in partly, on formation of the conjugates. In order to investigate the sequential metabolism of the dietary bioflavonoid in vitro, xenobiotic metabolizing enzymes, several human P450 and UGT were coexpressed in Saccharomyces cerevisiae AH22. These human P450 and UGT co-expression system in yeast allows us to investigate the sequential biotransformation of xenobiotics including dietary flavonoids to be simulated in vitro.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Quantitative analysis of UGT1A and UGT2B expression levels in human livers2009
Author(s)
Izukawa, T., Nakajima, M., Fujiwara, R., Yamanaka, H., Fukami, T., Takamiya, M., Aoki, Y., Ikushiro, S., Sakaki, T., Yokoi, T.
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Journal Title
Drug Metabolism and Disposition 37
Pages: 1759-1768
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