Function and the molecular mechanism of ascorbic acid in zone-specific steroidogenesis of the adrenal cortex

• Project/Area Number: 20580142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Keio University
• Principal Investigator: MITANI Fumiko Keio University, 医学部, 講師 (60041852)
• Co-Investigator(Kenkyū-buntansha): MUKAI Kuniaki 慶應義塾大学, 医学部, 助教 (80229913)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ステロイドホルモン / 生理活性物質 / 副腎皮質 / ビタミンC / P-450 / アルドステロン / アスコルビン酸 / 副賢皮質

Research Abstract

The level of near to 20mM ascorbic acid (Asc) in the adrenal gland is the highest in a body. The purpose of this study was to investigate the possible participation of Asc in the zone-specific steroidogenesis. By employing rat mutants unable to synthesize Asc, Asc was shown to be a reducing factor supporting the classical reducing system specifically in aldosterone synthesis in the zona glomerulosa (zG). One of the components in the regeneration system of Asc, OMb, was abundant in zG, which seems to support above results.

Research Products

• [Journal Article] Adrenocortical zonation in humans under normal and pathological conditions (2010)
  • Author(s): Nishimoto K, Nakagawa K, Dan L, Kosaka T, Oya M, Mikami S, Shibata H, Itoh H, Mitani F, Yamazaki T, Ogishima T, Suematsu M, Mukai K.
  • Journal Title: J Clin Endocrinol Metab 95 Pages: 2296-2305
  • Peer Reviewed
• [Journal Article] Adrenocortical Zonation in Humans under Normal and Pathological Conditions (2010)
  • Author(s): Nishimoto K, Mitani F, Mahal K, et al
  • Journal Title: J Clin Endocrinol Metab Volume: 95 Pages: 2296-2305
  • Peer Reviewed
• [Journal Article] Adrenocortical Zonation in Humans under Normal and Pathological Conditions (2010)
  • Author(s): Nishimoto K, et al.
  • Journal Title: J Clin Endocrinol Metab
  • Peer Reviewed
• [Journal Article] Cytochrome P450 (17alpha)in beta-cells of rat pancreas and its local steroidogenesis (2008)
  • Author(s): Ogishima T, Mitani F, Suematsu M.
  • Journal Title: J Steroid Biochem Mol Biol 111 Pages: 80-86
  • Peer Reviewed
• [Journal Article] Cytochrome P-450(17alpha)in beta-cells of rat pancreas and its local steroidogenesis (2008)
  • Author(s): Ogishima T, Mitani. F., Suematsu, M.
  • Journal Title: J. steroid Biochem. Mol. Biol. 111 Pages: 80-86
  • Peer Reviewed
• [Presentation] 2型糖尿病モデルラット副腎球状層におけるCYP17Aの発現 (2010)
  • Author(s): 三谷芙美子、荻島正、上村修平、向井邦晃, 他
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 2型糖尿病モデルラットのランゲルハンス島におけるステロイド合成酵素の発現 (2010)
  • Author(s): 荻島正、上村修平、三谷芙美子、向井邦晃, 他
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] ヒト副腎皮質における自律的アルドステロン産生細胞クラスター (2010)
  • Author(s): 西本紘嗣郎、三谷芙美子、向井邦晃, 他
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 2型糖尿病モデルラット副腎球状層におけるCYP17Aの発現 (2010)
  • Author(s): 三谷芙美子、荻島正、上村修平, et al
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-10
• [Presentation] 2型糖尿病モデルラットのランゲルハンス島におけるステロイド合成酵素の発現 (2010)
  • Author(s): 荻島正、上村修平、三谷芙美子, et al
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-10
• [Presentation] ヒト副腎皮質における自律的アルドステロン産生細胞クラスター (2010)
  • Author(s): 西本紘嗣郎、三谷芙美子、向井邦晃, et al.
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-10
• [Presentation] Variegated Zonation in Human Adrenal Cortex (2010)
  • Author(s): Nishimoto K., Mukai K., Ogishima T., et al.
  • Organizer: XIV Adrenal Cortex Conference, Adrenal 2010
  • Place of Presentation: Westin San Diego, USA
  • Year and Date: 2010-06-17
• [Presentation] Variegated zonation in human adrenal cortex (2010)
  • Author(s): Nishimoto K, Mukai K, Ogishima T., et al.
  • Organizer: XIV Adrenal Cortex Conference, Adrenal
  • Place of Presentation: San Diego, USA
• [Presentation] Adrenocortical zonation in humans under normal and pathological conditions (2009)
  • Author(s): Nishimoto K, Mukai K, Mitani F, et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] Localization of cytochrome P450 and production of local steroids in pancreatic β-cells (2009)
  • Author(s): Yoshino K, Ogishima T, Mitani F, et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] Adrenocortical Zonation in Humans under Normal and Pathological Conditions (2009)
  • Author(s): Nishimoto K, et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-21
• [Presentation] Localization of Cytochrome P450 and Production of Local Steroids in Pancreatic Beta-cells (2009)
  • Author(s): Yoshino K, et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-21
• [Presentation] (2009)
  • Author(s): Morizane S, Sano M, Moriyama H, Mitani F, et al.
  • Organizer: 日本循環器病学会2009
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-20
• [Presentation] Inadequate suppression of aldosterone biosynthesis after salt load is a primary cause of sodium-retention in Dahl salt-sensitive rat (2009)
  • Author(s): Morizane S, Sano M, Moriyama H, Matsuhashi T, Mitani F., et al.
  • Organizer: 日本循環器病学会2008
  • Place of Presentation: 大阪国際会議場
• [Presentation] 膵臓β-細胞が産生する局所ステロイドの合成と機能 (2008)
  • Author(s): 吉野和寿、山崎岳、三谷芙美子、向井邦晃, 他
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] A matricellular protein AZ-1 potentially functions as an endogenous angiostatic factor by antagonizing the angiogenic effects of VEGE (2008)
  • Author(s): Dan L, Mukai K, Suzuki R, Mitani F, Suematsu M
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 膵臓β-細胞が産生する局所ステロイドの合成と機能 (2008)
  • Author(s): 吉野和寿, 宗綱栄二, 山崎岳, 三谷芙美子, 向井邦晃, et al
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-10
• [Presentation] A matiricellular protein AZ-1 potentially functions as an endogenous angiostatic factor by antagonizing the angiogenic effects of VEGF (2008)
  • Author(s): Dan L, Mukai K, Suzuki R, Mitani F, Suematsu M
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-10
• [Book] イラストレイテッドハーパー・生化学(原書28版) (2011)
  • Author(s): 丸善株式会社
  • Publisher: 上代、清水監訳、訳者:清水、市山、三谷, 他
• [Book] イラストレイテッド ハーパー・生化学(原書28版) (2011)
  • Author(s): Murray RK., Granner DK., Mayes PA., Rodwell VW(上代、清水監訳、訳者:上代、市山、三谷, et al.)
  • Total Pages: 812
  • Publisher: 丸善株式会社