Analysis of peanut allergen with human monoclonal antibodies

• Project/Area Number: 20580143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Tamagawa University
• Principal Investigator: SHINMOTO Hiroshi Tamagawa University, 農学部, 教授 (50355301)
• Research Collaborator: SOHEILA J.Maleki 米国農務省農業研究所南部研究センター
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 食物アレルギー / ピーナツ / エピトープ / モノクローナル抗体 / アミノ酸配列 / ペプチド合成 / 抗原提示細胞 / IgM / アレルギー / リンパ球 / エピトープ解析 / 形質転換 / 抗体

Research Abstract

Peripheral blood B-cells were transformed with Epstein-Barr virus. We have obtained 31 oligo-clonal B-cells secreting antibodies recognizing 6 parts or peanut allergen Ara h1 protein. Detailed analysis with human IgM class monoclonal antibodies 92-2 and #86 against peanut allergen Ara h1 showed that the peptides QEWGTPGS and DEDEEEE were core epitopes of Ara h1.

Research Products

• [Journal Article] Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody clone #86. (2011)
  • Author(s): 新本洋士
  • Journal Title: Hum Antibodies. Volume: 19 Pages: 101-105
  • Peer Reviewed
• [Journal Article] Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody clone #86. (2010)
  • Author(s): Shinmoto H, Takeda M, Matsuo Y, Naganawa Y, Tomita S, Takano-Ishikawa Y.
  • Journal Title: Hum Antibodies. 19 Pages: 101-105
  • Peer Reviewed
• [Journal Article] Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2. (2010)
  • Author(s): Shinmoto H, Matsuo Y, Naganawa Y, Tomita S, Takano-Ishikawa Y.
  • Journal Title: Cytotechnology. 62 Pages: 307-311
  • Peer Reviewed
• [Journal Article] Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2 (2009)
  • Author(s): Shinmoto H.
  • Journal Title: Cytotechnology (online first version) (online)
  • Peer Reviewed
• [Journal Article] Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells. (2008)
  • Author(s): Naganawa Y, Shimmoto M, Maleki SJ, Takase M, Shinmoto H.
  • Journal Title: Cytotechnology. 57 Pages: 177-180
  • Peer Reviewed
• [Journal Article] Epitope analysis of peanut allergen Ara hl with oligo-clonal IgM antibody from human B-lymphoblastoid cells (2008)
  • Author(s): Y. Naganawa, et al.
  • Journal Title: Cytotechnology 57 Pages: 177-180
  • Peer Reviewed
• [Presentation] 抗ピーナツアレルゲン Ara h1ヒトモノクローナル抗体 (2009)
  • Author(s): 新本洋士
  • Organizer: 日本動物細胞工学会 つくば大会 2009
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2009-07-25
• [Presentation] Epitope analysis of peanut allergen Ara hl with human monoclonal IgM antibody 92-2 (2008)
  • Author(s): H. Shinmoto, et al.
  • Organizer: The 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2008-11-26
• [Book] 食物アレルギーA to Z (2010)
  • Author(s): 新本洋士,中村他編著
  • Publisher: 第一出版