Enantioseparation of DL-galactose in plants and foods

• Project/Area Number: 20580148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Toyama Institute of Health
• Principal Investigator: KODAMA Shuji Toyama Institute of Health, 化学部, 主幹研究員 (70360807)
• Co-Investigator(Kenkyū-buntansha): KEMMEI Tomoko 富山県衛生研究所, 化学部, 副主幹研究員 (60416089) ; YAMAMOTO Atsushi 中部大学, 応用生物学部, 教授 (60360806) ; AIZAWA SenーIchi 富山大学, 理工学研究部, 教授 (60231099) ; TAGA Atsushi 近畿大学, 薬学部, 講師 (20247951)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: キャピラリー電気泳動 / 光学分割 / 単糖 / L-糖 / 配位子交換

Research Abstract

D-Monosaccharides are distributed widely in nature, but it is not clarified that free L-monosaccharides, such as L-galactose, L-glucose and L-mannose, present. We tried to enantioseparate galactose in vegetables and processed foods by chiral ligand-exchange capillary electrophoresis. And also, epimerization of galactose was studied.

Research Products

• [Journal Article] Enantioseparation of DL-isocitric acid by a chiral ligand exchange capillary electrophoresis with Ni(II)-D-quinic acid system. (2010)
  • Author(s): Kodama, S., Taga, A., Yamamoto, A., Ito, Y., Honda, Y., Suzuki, K., Yamashita, T., Kemmei, T., Aizawa, S
  • Journal Title: Electrophoresis 31 Pages: 3586-3591
  • Peer Reviewed
• [Journal Article] In vitro assay of the interaction between Rnc1 protein and Pmp1 mRNA by affinity capillary electrophoresis with a carboxylated capillary (2010)
  • Author(s): Taga, A., Satoh, R., Ishiwata, S., Kodama, S., Sato, A., Suzuki, K., Sugiura R
  • Journal Title: J. Pharm. Biomed.Anal. 53 Pages: 1332-1337
  • Peer Reviewed
• [Journal Article] Analysis of phosphorus-containing amino acid-type herbicides by capillary electrophoresis/ mass spectrometry using a chemically modified capillary having amino groups (2010)
  • Author(s): Iwamuro, Y., Iio-Ishimaru, R., Chinaka, S., Takayama, N., Kodama, S., Hayakawa, K
  • Journal Title: J. Heath Sci 56 Pages: 606-612
  • NAID: 130000425097
  • Peer Reviewed
• [Journal Article] Metal(II)-Ligand molar ratio dependence of enantioseparation of tartaric acid by ligand exchange capillary electrophoresis with Cu(II) and Ni(II)-D-quinic acid systems. (2010)
  • Author(s): Kodama, S., Aizawa, S., Taga, A., Yamashita, T., Kemmei, T., Suzuki, K., Honda, Y., Yamamoto, A
  • Journal Title: Electrophoresis 31 Pages: 1051-1054
  • Peer Reviewed
• [Journal Article] Reproducible chiral capillary electrophoresis of methanephetamine and its related compounds using a chemically modified capillary having diol groups (2010)
  • Author(s): Iwamuro, Y., Iio-Ishimaru, R., Chinaka, S., Takayama, N., Kodama, S., Hayakawa, K.
  • Journal Title: Forensic Toxicol 28 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Enanti oseparation of DL-isocitric acid by a chiral ligand exchange capillary electrophoresis with Ni(II)-D-quinic acid system (2010)
  • Author(s): Kodama, et al.
  • Journal Title: Electrophoresis Volume: 31 Pages: 3586-3591
  • Peer Reviewed
• [Journal Article] In vitro assay of the interaction between Rncl protein and Pmpl mRNA by affinity capillary electrophoresis with a carboxylated capillary (2010)
  • Author(s): Taga, et al.
  • Journal Title: J.Pharm.BiomedAnal. Volume: 53 Pages: 1332-1337
  • Peer Reviewed
• [Journal Article] Metal(II)-ligand molar ratio dependence of enantioseparation of tartaric acid by ligand exchange capillary electrophoresis with Cu(II) and Ni(II) -D-quinic acid systems (2010)
  • Author(s): Shuji Kodama, et al.
  • Journal Title: Electrophoresis 31 Pages: 1051-1054
  • Peer Reviewed
• [Journal Article] Evaluation of sulfobetaine-type polymer resin as an SPE adsorbent in the analysis of trace tetracycline antibiotics in hone (2009)
  • Author(s): Tsukamoto, T., Yasuma, M., Yamamoto, A., Hirayama, K., Kihou, T., Kodama, S., Inoue, Y.
  • Journal Title: J. Sep. Sci 32 Pages: 3591-3595
  • Peer Reviewed
• [Journal Article] Enan-tioseparation of nicotine alkaloids in cigarettes by capillary electrophoresis using sulfated β-cyclodextrin as a chiral selector and a capillary coated with amino groups (2009)
  • Author(s): Kodama, S., Morikawa, A., Nakagomi, K., Yamamoto, A., Sato, A., Suzuki, K., Yamashita, T., Kemmei, T., Taga A.
  • Journal Title: Electrophoresis 30 Pages: 349-356
  • Peer Reviewed
• [Journal Article] Study of solid-phase extraction for the determination of sequestering agents in river water by high-performance liquid chromatography (2009)
  • Author(s): Kemmei, T., Kodama, S., Muramoto, T., Fujishima, H., Yamamoto, A., Inoue, Y., Hayakawa, K
  • Journal Title: J. Chromatogr. A 1216 Pages: 1109-1114
  • Peer Reviewed
• [Journal Article] Evaluation of sulfobetaine-type polymer resin as an SPE adsorbent in the analysis of trace tetracycline antibiotics in honey (2009)
  • Author(s): Tomoyasu Tsukamoto, et al.
  • Journal Title: Journal of Separation Science 32 Pages: 3591-3595
  • Peer Reviewed
• [Journal Article] Hayakawa K. A fast and simple analysis of glyphosate in tea beverages by capillary electrophoresis with on-line copper(II)-glyphosate complex formation (2008)
  • Author(s): Kodama, S., Ito, Y., Taga, A., Nomura, Y., Yamamoto, A., Chinaka, S., Suzuki, K.. Yamashita, T., Kemmei, T.
  • Journal Title: J. Health Sci 54 Pages: 602-606
  • Peer Reviewed
• [Journal Article] Determination of adulteration in apple juice by HPLC with novel optical rotation detector (2008)
  • Author(s): Yamamoto, A., Kawai, M., Miwa, T Tsukamoto, T., Kodama, S., Hayakawa, K.
  • Journal Title: J Agric. Food Chem 56 Pages: 7302-7304
  • Peer Reviewed
• [Journal Article] Analysis of an antibody pharmaceutical, tocilizumab by capillary electrophoresis using a carboxylated capillary. (2008)
  • Author(s): Taga, A., Kita, S., Nishiura, K., Hayashi, T.,Kinoshita, M., Sato, A., Suzuki, K., Kodama, S., Kakehi, K.
  • Journal Title: J. Sep. Sci. 31 Pages: 853-858
  • Peer Reviewed
• [Journal Article] An optical rotatory detector for high-performance liquid chromatography using polarization modulation (2008)
  • Author(s): Yamamoto.A., Kawai, M., Sakamoto, Kodama, S., Hayakawa, K.
  • Journal Title: Anal. Sci 24 Pages: 361-364
  • NAID: 10021099026
  • Peer Reviewed
• [Journal Article] A fast and simple analysis of glyphosate in tea beverages by capillary electrophoresis with on-line copper(II)-glyphosate complex formation (2008)
  • Author(s): Shuji Kodama, et al.
  • Journal Title: Journal of Health Science 54 Pages: 602-606
  • NAID: 110006935590
  • Peer Reviewed
• [Journal Article] Deermination of adulteration in apple juice by HPLC with novel optical rotation detector (2008)
  • Author(s): Atsushi Yamamoto, et al.
  • Journal Title: Journal of Agricultural and Food Chemistry 56 Pages: 7302-7304
  • Peer Reviewed
• [Journal Article] Analysis of an antibody pharmaceutical, tocilizumab, by capillary electrophoresis using a carboxylated capillary (2008)
  • Author(s): Atsushi Taga, et al.
  • Journal Title: Journal of Separation Sciences 31 Pages: 853-858
  • Peer Reviewed
• [Presentation] 還元糖光学異性体の加熱による異性化 (2010)
  • Author(s): 小玉修嗣, 他
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学川内北キャンパス
• [Presentation] キラル配位子交換キャピラリー電気泳動によるイソクエン酸の光学異性体分析 (2010)
  • Author(s): 小玉修嗣, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
• [Presentation] アミノ基導入化学修飾キャピラリーを用いたキャピラリー電気泳動/質量分析(CE・MS)法による含リンアミノ酸系農薬の分析 (2010)
  • Author(s): 岩室嘉晃, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
• [Presentation] 回折格子一次光の遅相測定-新規旋光度検出器の感度に及ぼす遅相の影響 (2010)
  • Author(s): 三輪俊夫, 他
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ
• [Presentation] 還元糖光学異性体の加熱による異性化 (2010)
  • Author(s): 小玉修嗣
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学川内 キャンパス(仙台市)
• [Presentation] キラル配位子交換キャピラリー電気泳動法によるイソクエン酸の光学異性体分析 (2010)
  • Author(s): 小玉修嗣
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山大学津島キャンパス、桃太郎アリーナ他(岡山市)
• [Presentation] キャピラリー電気泳動/質量分析法の法科学分析への有効利用-化学修飾キャピラリーによる迅速性と再現性の向上- (2009)
  • Author(s): 岩室嘉晃, 他
  • Organizer: 第29回キャピラリー電気泳動シンポジウム
• [Presentation] 配位子交換キャピラリー電気泳動法での配位子濃度依存的エナンチオマー泳動順の逆転現象 (2009)
  • Author(s): 小玉修嗣, 他
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学高等教育機能開発総合センター
• [Presentation] 新規旋光度検出器を用いた光学純度測定～マツタケオールにおける溶媒増感効果～ (2009)
  • Author(s): 三輪俊夫, 他
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学高等教育機能開発総合センター
• [Presentation] 中心イオンにNi(II)イオンを用いたキラル配位子交換キャピラリー電気泳動法 (2009)
  • Author(s): 小玉修嗣, 他
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] キャピラリー電気泳動法によるメープルシロップおよびメープルシュガー中の還元糖の分析 (2009)
  • Author(s): 多賀淳, 他
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] キラル検出器による抗真菌薬イトラコナゾールの光学分割 (2009)
  • Author(s): 三輪俊夫, 他
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 河川水中のグリホサートおよびアミノメチルリン酸の簡易分析 (2009)
  • Author(s): 野村陽子, 他
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 配位子交換キャピラリー電気泳動法での配位子濃度依存的エナンチオマー泳動順の逆転現象 (2009)
  • Author(s): 小玉修嗣
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学高等教育機能開発総合センター(札幌市)
• [Presentation] キャピラリー電気泳動によるメープルシロップ及びメープルシュガー中の還元糖の分析 (2009)
  • Author(s): 多賀淳, 他2名
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 中心イオンにNi(II)イオンを用いたキラル配位子交換キャピラリー電気泳動 (2009)
  • Author(s): 小玉修嗣, 他6名
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] オンライン銅錯体形成を利用した茶飲料混入グリホサートの迅速キャピラリー電気泳動分析 (2008)
  • Author(s): 小玉修嗣, 他
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学七隈キャンパス
• [Presentation] 新規旋光度検出器の開発-CD検出器との比較- (2008)
  • Author(s): 三輪俊夫, 他
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学七隈キャンパス
• [Presentation] 食品分析におけるダイアリシス前処理信頼性の確認 (2008)
  • Author(s): 中島康夫, 他
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学七隈キャンパス