Project/Area Number |
20580168
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Forest science
|
Research Institution | Forestry and Forest Products Research Institute |
Principal Investigator |
OHMIYA Yasunori Forestry and Forest Products Research Institute, 林木育種センター東北育種場, 主任研究員 (70360469)
|
Co-Investigator(Kenkyū-buntansha) |
AKADA Shinji 弘前大学, 遺伝子実験施設, 准教授 (10250630)
UEMURA Matsuo 岩手大学, 農学部付属寒冷バイオフロンティア研究センター・生命適応機能研究分野, 教授 (00213398)
|
Co-Investigator(Renkei-kenkyūsha) |
SAITO Hideyuki 北海道大学, 農学研究科, 助教 (70312395)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 遺伝子 / 樹木 / 発現制御 / 森林工学 / ブナ / 植物 |
Research Abstract |
We have isolated Arabidopsis orthologs, constans (CO) and flowering locus T (FT) genes from Japanese beech tree, which are mainly function in flower transition, and their full sequences were identified. Real-Time PCR analysis revealed that CO transcripts are closely related to flowering initiation with high expression level of CO mRNAs during the period in May, a year before flowering. Transgenic Arabidopsis overexpressing CO product represented early flowering phenotype, this is a result of revealing its flowering enhancement function of CO. Their comparisons of nucleotide sequences were performed among 38 clones collected from Tohoku area. It seemed likely that there are possibilities of insertion/deletion including nucleotide polymorphisms (SNPs) within several FT genes that might influence its function, while there are no differences among CO sequences. Further analyses of FT including SNPs and producing overexpressing lines are ongoing.
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