Improvement of the saprophytic ability in Matsutake mushroom by cross breeding technology

• Project/Area Number: 20580175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Tottori University
• Principal Investigator: AIMI Tadanori Tottori University, 農学部, 教授 (90264928)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: マツタケ / プロトプラスト / グルコアミラーゼ遺伝子 / 育種 / 一核株 / 核相 / 再生 / ホメオドメインタンパク質 / 交配型 / ホメオドメイン / 生態型 / ミトコンドリア / rDNA / 宿主 / 広葉樹 / 針葉樹

Research Abstract

Several genes related for mating type and starch metabolism were identified to use for investigation of mating type and karyotype in the T.matsutake isolates. From these results indicated that the T.matsutake isolates from the fruiting body tissue were essentially heterokaryon. Secondly, we examined the condition of protoplast formation and its regeneration in order to isolate homokaryon in the ectomycorrhizal fungus Tricholoma matsutake.
As the results, when trehalose was used as osmotic stabilizer, the protoplast formation and mycelial growth of T.matsutake was promoted, however, xylose completely inhibited mycelial growth.

Research Products

• [Journal Article] アジアのマツタケおよびマツタケ近縁種の宿主樹木
  • Author(s): 山中勝次・曹暉・陳明悉・万佳寧・会見忠則
  • Journal Title: 日本きのこ学会誌 印刷中
  • NAID: 110008711282
• [Presentation] Distinguishing karyotype in Tricholoma matsutake. (2011)
  • Author(s): Wan J., Yi R., Li Y., Masuda K., Yamanaka K., Yamaguchi T., Shimomura N., Aimi T.
  • Organizer: The 7th International Conference on Mushroom Biology and Mushroom Products,(発表確定)
  • Place of Presentation: Arcachon
  • Year and Date: 2011-10-06
• [Presentation] Cloning, characterization and expression of glucoamylase gene from ectomycorrhizal basidomycete, Tricholoma matsutake. (2010)
  • Author(s): Wan J., Yi R., LI Y., Kinjo Y., Terashita T., Yamanaka K., Aimi T.
  • Organizer: The 6th Meeting of East Asia for Mushroom Science
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2010-11-13
• [Presentation] Cloning, characterization and expression of glucoamylase gene from ectomycorrhizal basidomycete, Tricholoma matsutake (2010)
  • Author(s): Tadanori Aimi
  • Organizer: The 6th Meeting of East Asia for Mushroom Science
  • Place of Presentation: Gyeongju TEMF Hotel (Gyeongju、Korea)
  • Year and Date: 2010-11-13
• [Presentation] マツタケの(Tricholoma matsutake)のグルコアミラーゼ遺伝子のクローニングと発現解析 (2010)
  • Author(s): 万佳寧・蟻瑞栄・李燕・寺下隆夫・山中勝次・山口武視・會見忠則
  • Organizer: 日本きのこ学会第14回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-17
• [Presentation] マツタケの(Tricholoma matsutake)のグルコアミラーゼ遺伝子のクローニングと発現解析 (2010)
  • Author(s): 会見忠則
  • Organizer: 日本きのこ学会第14回大会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京都文京区)
  • Year and Date: 2010-09-16
• [Presentation] マツタケ及びホンシメジのグルコアミラーゼ遺伝子の解析 (2009)
  • Author(s): 貞島亜紀・寺下隆夫・山中勝次・会見忠則
  • Organizer: 日本きのこ学会第13回大会
  • Place of Presentation: 兵庫県西宮市
  • Year and Date: 2009-09-10
• [Presentation] ブータンのマツタケ (2008)
  • Author(s): 山中勝次, 持田裕介, 会見忠則
  • Organizer: 日本菌学会第52回大会
  • Place of Presentation: 三重県津市
  • Year and Date: 2008-06-01