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Studies on mechanism of lipid uptake into teleost oocytes

Research Project

Project/Area Number 20580192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

TODO Takashi  Hokkaido University, 大学院・水産科学研究院, 准教授 (60303111)

Co-Investigator(Renkei-kenkyūsha) HARA Akihiko  北海道大学, 大学院・水産科学研究院, 教授 (40091483)
HIRAMATSU Naoshi  北海道大学, 大学院・水産科学研究院, 助教 (10443920)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords卵形成 / 卵成長 / 卵母細胞 / 油球 / 脂質 / リポタンパク / サケ科魚類 / 水産学 / 発生・分化
Research Abstract

In teleost fishes, high amounts of lipids and proteins are accumulated in oocytes during their growth phase ; they are later utilized as energy and nutrient resources by developing embryos and larvae. However, little is known about the origin of such lipids and the mechanisms underlying their accumulation into oocytes. In the present study, we aimed to clarify the mechanisms at the molecular levels. As the results, we identified various factors involved in the lipid uptake into fish oocytes, and proposed a new model for the oocyte lipidation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Androgens and very low density lipoprotein are essential for the growth of previtellogenic oocytes from Japanese eel, Anguilla japonica, in vitro2011

    • Author(s)
      Taku Endo、Takashi Todo、P.Mark Lokman、Hideaki Kudo、Shigeho Ijiri、Shinji Adachi、Kohei Yamauchi
    • Journal Title

      Biology of Reproduction Vol.84

      Pages: 816-825

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Androgens and very low density lipoprotein are essential for the growth of previtellogenic oocytes from Japanese eel, Anguilla japonica, in vitro2011

    • Author(s)
      Taku Endo
    • Journal Title

      Biology of Reproduction

      Volume: 84 Pages: 816-825

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of reproductive stage and 11-ketotestosterone on LPL mRNA levels in the ovary of the shortfinned eel2010

    • Author(s)
      Sean L.Divers、H.James McQuillan、Hajime Matsubara、Takashi Todo、P.Mark Lokman
    • Journal Title

      Journal of Lipid Research Vol.51

      Pages: 3250-3258

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of androgen receptor mRNA in the ovary of Japanese eel, Anauilla japonica, during artificially induced ovarian development2010

    • Author(s)
      Ryota Tosaka
    • Journal Title

      Genereal and Comparative Endocrinology

      Volume: 168 Pages: 424-430

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of reproductive stage and 11-ketotestosterone on LPL mRNA levels in the ovary of the shortfinned eel2010

    • Author(s)
      Sean L.Divers
    • Journal Title

      Journal of Lipid Research

      Volume: 51 Pages: 3250-3258

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] カットスロートトラウト卵巣におけるリポタンパクリパーゼファミリー遺伝子の発現解析2010

    • Author(s)
      柳蓉法
    • Organizer
      第4回サケ学研究会
    • Place of Presentation
      北海道大学水産学部(函館市)
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Studies on molecular mechanisms of lipid uptake into fish oocytes:identification and expression analysis of lipoprotein family genes in the ovary of cutthroat trout, Oncorhynchus clarki2010

    • Author(s)
      Yongwoon Ryu
    • Organizer
      The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Place of Presentation
      Jeju National University(大韓民国)
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] カットスロートトラウト卵巣におけるリポプロテインリパーゼファミリー遺伝子の発現解析2010

    • Author(s)
      柳蓉〓
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] カットスロート卵巣におけるビテロジェニン受容体蛋白質の性状解析2010

    • Author(s)
      水田紘子
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] カットスロートトラウト卵巣におけるリポタンパクリパーゼファミリー遺伝子の発現解析2010

    • Author(s)
      柳蓉〓
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] カットスロートトラウト卵巣におけるビテロジェニン受容体蛋白質の性状解析2010

    • Author(s)
      水田紘子
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学生物資源科学部(藤沢市)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] カットスロートトラウト卵巣におけるリポプロテインリパーゼのcDNAクローニングと発現解析2009

    • Author(s)
      東藤孝
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] カットスロートトラウト低密度リポ蛋白受容体(LDLR)遺伝子の発現解析2009

    • Author(s)
      伊東優太
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都品川区)
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] カットスロート卵巣におけるリポタンパクリパーゼのcDNAクローニングと発現解析2009

    • Author(s)
      東藤孝
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] カットスロート低密度リポ蛋白受容体(LDLR)遺伝子の発現解析2009

    • Author(s)
      伊東優太
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Changes in ovarian lipoprotein lipase transcript abundance in the New Zealand shortfinnedeel Anguilla austraris during oogenesis and in response to 11-ketotestosterone2008

    • Author(s)
      Divers, S. L.
    • Organizer
      6th International Symposium on Fish Endocrinology
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2008-06-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] Teleost oocyte growth, vitellogenesis, and cytoplasmic maturation2008

    • Author(s)
      Sullivan, C.V.
    • Organizer
      Int. Symp. in Honor of Prof. Y. Nagahama, Sex Determination and Gametogenesis in Fish
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2008-05-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] Expression of lipoprotein lipase gene in ovarian follicles of cutthroat trout (Oncorhynchus clarkii) during gonadal development2008

    • Author(s)
      Todo, T
    • Organizer
      Int. Symp. in Honor of Prof. Y. Nagahama, Sex Determination and Gametogenesis in Fish
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2008-05-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular cloning and characterization of low density lipoprotein receptor in cutthroat trout (Oncorhynchus clarkii)2008

    • Author(s)
      Massaki, K.
    • Organizer
      Int. Symp. in Honor of Prof. Y. Nagahama, Sex Determination and Gametogenesis in Fish
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2008-05-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] Yolk assembly in teleost : recent findings on the deposition of ovarian lipids and proteins2008

    • Author(s)
      Naoshi Hiramatsu
    • Organizer
      World Aquaculture
    • Place of Presentation
      大韓民国・釜山
    • Year and Date
      2008-05-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Yolk assembly in teleost : recent findings on the deposition of ovarian lipids and proteins2008

    • Author(s)
      Hiramatsu, N.
    • Organizer
      World Aquaculture
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2008-05-23
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.geocities.co.jp/hlaboratory/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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