Characterization of type 1 interferon in common carp Cyprinus carpio

• Project/Area Number: 20580206
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: University of Miyazaki
• Principal Investigator: SAKAI Masahiro University of Miyazaki, 農学部, 教授 (20178536)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: インターフェロン / コイ / 遺伝子 / TLR / 発現解析 / 発現 / Mx / イムキモット / LPS / 構造

Research Abstract

The type I interferon (I-IFN) gene has recently been cloned and sequenced in the common carp species Cyprinus carpio L. Carp I-IFN cDNA is composed of 675 base pairs and is translated into a protein of 186 amino acid residues. The carp I-IFN encodes a predicted signal peptide of 23 amino acid residues and contains the I-IFN family signature His^<140>-Trp^<158>. Analysis of the homology between carp I-IFN and other known I-IFN and type II interferon (II-IFN) family members has revealed significant similarities to grass carp I-IFN. Phylogenetic analysis demonstrated that carp I-IFN clusters with I-IFN in teleosts, away from the other II-IFN family members. In addition, the gene structure for carp I-IFN is composed of 5 exons and 4 introns, a composition that is similar to that of the teleost I-IFN gene. RT-PCR analysis did not reveal gene expression in un-stimulated tissues including intestine, liver, gill, head kidney, muscle, spleen, mid-kidney and skin. However, I-IFN expression levels increased following stimulation with imiquimod in the head kidney cells. Furthermore, recombinant carp I-IFN protein (mature form) produced via the cell-free protein synthesis system stimulated the expression of the interferon-inducible Mx gene in the head kidney cells.

Research Products

• [Journal Article] Cloning, characterization, and expression analysis of Toll-like receptor-7 cDNA from common carp Cyprinus carpio. (2010)
  • Author(s): Tanekhy, M., Kono, M., Sakai, M.
  • Journal Title: Comparative Biochemistry and Physiology D 5 Pages: 245-255
  • Peer Reviewed
• [Journal Article] Cloning, characterization, and expression analysis of Toll-like receptor-7cDNA from common carp Cyprinus carpio (2010)
  • Author(s): Tanekhy, M., Kono, M., Sakai, M.
  • Journal Title: Comparative Biochemistry and Physiology D : Genomics and Proteomics Volume: 5 Pages: 245-255
  • Peer Reviewed
• [Journal Article] Characterization and expression analysis of type 1 interferon in common carp Cyprinus carpio. (2009)
  • Author(s): Kitao, Y., Kono, T., Korenaga, H., Iizasa, T., Nakamura, K., Savan, R., Sakai, M.
  • Journal Title: Molecular Immunology 46 Pages: 2548-2556
  • Peer Reviewed
• [Journal Article] Characterization and expression analysis of type 1 interferon in common carp Cyprinus carpio (2009)
  • Author(s): Kitao, Y., Kono, T., Korenaga, H., Iizasa, T., Nakamura, K., Savan, R., Sakai, M.
  • Journal Title: Molecular Immunology 46 Pages: 2548-2556
  • Peer Reviewed
• [Presentation] Cytokine responses in carp kidney leucocytes treated by LPS, PolyI : C and imiqumod. (2010)
  • Author(s): Tanekhy, M., Kono, T., Sakai, M.
  • Organizer: First EOFFI Symposium
  • Place of Presentation: ビテルボ、イタリア
  • Year and Date: 2010-05-24
• [Presentation] Characterization and expression analysis of type 1 interferon in common carp Cyprinus carpio. (2009)
  • Author(s): Kitao, Y., Kono, T., Sakai, M.
  • Organizer: 26^<th> ESCPB Congress
  • Place of Presentation: インスブルック、オーストリア
  • Year and Date: 2009-09-06
• [Presentation] Characterization and expression analysis of type 1 interferon in common carp Cyprinus carpio (2009)
  • Author(s): Kitao, Y., Kono, T., Sakai, M.
  • Organizer: 26^<th> ESCPB Congress
  • Place of Presentation: インスブルック、オーストリア
  • Year and Date: 2009-09-06
• [Presentation] Two isoforms of type1 interferon gene in the common carp Cyprinus carpio L. (2008)
  • Author(s): Sakai, M., Kitao, Y., Kono, T.
  • Organizer: 5^<th> World Fisheries Congress
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-22
• [Presentation] Two isoforms of type1 interferon geneisolated from the common carp Cyprinus carpio L. (2008)
  • Author(s): Kitao, Y., Kono, T., Sakai, M.
  • Organizer: 25^<th> ESCPB Congress
  • Place of Presentation: ラベンナ、イタリア
  • Year and Date: 2008-09-11
• [Presentation] Two isoforms of type I interferon gene isolated from the common carp Cyprinus carpio L. (2008)
  • Author(s): Kitao, Y., Kono, T., Sakai, M.
  • Organizer: 25^<th> ESCPB Congress
  • Place of Presentation: ラベンナ イタリア
  • Year and Date: 2008-09-08
• [Presentation] Characterization and expression analysis of two isoforms of type1 interferon in the common carp Cyprinus carpio L. (2008)
  • Author(s): Kitao, Y., Kono, T., Sakai, M.
  • Organizer: The 7^<th> symposium on Diseases in Asian Aquacuture
  • Place of Presentation: 台北、台湾
  • Year and Date: 2008-06-25
• [Remarks] ホームページ等