A study on fish lipocalin protein which bind to low molecular lipophilic compound

• Project/Area Number: 20580221
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kyushu University
• Principal Investigator: YUJI Oshima Kyushu University, 農学研究院, 教授 (70176874)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 静菌 / 生体防御 / 低分子結合性 / リポカリン / 静菌活性 / ヒラメ / 遺伝子組換え体 / 遺伝子組替え / シデロフォア

Research Abstract

Tributyltin (TBT) is well-known as a strong endocrine disruptor and has been detected at high concentrations in the blood of fishes living in coastal areas. In our previous studies, we identified TBT-binding proteins (TBT-bp1 and 2) bound to TBT in the blood of Japanese flounder (Paralichthys olivaceus) and determined that TBT-bps were have a function that would bind to TBT and then be excreted into the mucus. TBT-bps are a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1and -bp2 (rTBT-bp1, 2) in a baculovirus expression system and purified the protein from the hemolymph of silkworm larvae injected with recombinant baculovirus. From the results of binding assay of rTBT-bp 2 to some chemicals, TBT-bp2 show affinity to TBT but not to lauric acid, palmitic acid and tearic acid. Furthermore, rTBT-bp1 may inhibit the growth of fish dieseas bacteria Edwardsiella tarda, suggesting bacteriostasis activity.

Research Products

• [Journal Article] Purification and characterization of tributyltin-binding protein of tiger puffer (2011)
  • Author(s): Oba Y, Yamauchia A, Hashiguchi Y, Satonea H, Miki S, Nassef M, Shimasaki Y, Kitano T, Nakao M, Kawabata S, Honjo T, Oshima Y,.
  • Journal Title: Takifugu rubripes. Comp Biol phys C. 153 Pages: 17-23
• [Journal Article] Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. (2011)
  • Author(s): Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y.
  • Journal Title: Aquat Toxicol 103 Pages: 79-84
• [Journal Article] Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes. (2011)
  • Author(s): Nassef M, Kato-Unoki Y, Furuta T, Nakayama K, Satone H, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Zoological Science 28 Pages: 281-285
• [Journal Article] Purification and charac-terization of tributyltin-binding protein of tiger puffer, Takifugu rubripes. (2011)
  • Author(s): Oba Y, Yamauchia A, Hashiguchi Y, Satonea H, Miki S, Nassef M, Shimasaki Y, Kitano T, Nakao M, Kawabata S, Honjo T, Oshima Y
  • Journal Title: Comparative Biology and Physiology part C Volume: 153 Pages: 17-23
  • Peer Reviewed
• [Journal Article] Molecular cloning, sequencing, and gene expression analysis of tributyltin-binding protein type 1 in Japanese medaka fish, Oryzias latipes. (2011)
  • Author(s): Nassef M, Kato-Unoki Y, Furuta T, Nakayama K, Satone H, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Zoological Science Volume: 28(印刷中)
  • Peer Reviewed
• [Journal Article] Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27. (2011)
  • Author(s): Nassef M, Tawaratsumida T, oba Y, Satoe H, Nakayama K, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Marine Pollution Bulletin Volume: 62 Pages: 412-414
  • Peer Reviewed
• [Journal Article] Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. (2011)
  • Author(s): Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y
  • Journal Title: Aquatic Toxicology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Ovo nanoinjection of nonylphenol affects embryonic development of olvas-GFP/ST-II YI medaka, Oryzias latipes (2009)
  • Author(s): Hano T, Kinoshita M, Tanaka M, Y.W, Ozato O, Shimasaki Y, Oshima Y, Honjo T.
  • Journal Title: Chemosphere 77 Pages: 1594-1599
  • Peer Reviewed
• [Journal Article] Pharmaceuticals and personal care products toxicity to medaka fish (2009)
  • Author(s): Nassef M, Matsumoto S, Seki M, Kang IJ, Moroishi J, Shimasaki Y, Oshima Y.
  • Journal Title: J.Fac.Agr.Kyushu Univ. 55 Pages: 407-412
• [Journal Article] (2009)
  • Author(s): Oshima Y, Nakayama K, Hano T, Kim SG, Shimasaki Y, Kang IJ, Honjo T
  • Journal Title: Toxic interactions between tributyltin and polychlorinated biphenyls in aquatic organisms(Springer)2009(Ed T.Arai et al(eds.), In "Environmental Impact of Antifouling Biocides) Pages: 195-205
• [Journal Article] Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder (2008)
  • Author(s): Satone H, Oshima Y, Shimasaki Y, Tawaratsumida T, Oba Y, Takahashi E, Kitano T, Kawabata S, Kakuta Y, Honjo T.
  • Journal Title: Paralichthys olivaceus. Aquat Toxicol 90 Pages: 292-299
• [Journal Article] Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus. (2008)
  • Author(s): Satone, H., Oshima, Y., Shimasaki, Y., Tawaratsumida, T., Oba, Y., Takahashi, E., Kitano, T., Kawabata, S., Kakuta, Y., Honjo, T.
  • Journal Title: Aquatic Toxicology 90 Pages: 292-299
  • Peer Reviewed
• [Journal Article] Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27.
  • Author(s): Nassef M, Tawaratsumida T, Oba Y, Satoe H, Nakayama K, Shimasaki Y, Honjo T, Oshima Y.
  • Journal Title: Mar Poll Bull 62 Pages: 412-414
• [Journal Article] In ovo nanoinjection of triclosan, diclofenac and carbamazepine affects embryonic development of medaka fish(Oryzias latipes)
  • Author(s): Nassef M, Kim SG, Seki M, Kang IJ, Hano T, Shimasaki Y, Oshima Y
  • Journal Title: Chemosphere (Accepted, 印刷中)
  • Peer Reviewed
• [Presentation] 魚類リポカリンは異物(トリブチルスズやフグ毒)と結合し体表粘液から排泄する機能を持つ (2010)
  • Author(s): 大嶋雄治
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-16
• [Presentation] The detoxicification and excreation function of tributyltin binding protein in Japneseflounder Paralichthyes olivaceus. (2010)
  • Author(s): Oshima Y, Satone H, Yumi Oba Y, Lee JM, Kusakabe T, Kawabata S, Shimasaki Y, Suzuki N, Sasayama Y.
  • Organizer: Society of Environmental Toxicology and Chemistry Asia Pacific 2010
  • Place of Presentation: 広州(中華人民共和国)
  • Year and Date: 2010-06-04
• [Presentation] Induction of the tributyltin-binding protein 2 of Japanese flounder, Paralichthys olivaceus by exposure of tributyltin-d27. (2010)
  • Author(s): Y Oshima, T.Tawaratsumida, Y Oba, H Satone, Y Shimasaki
  • Organizer: 6^<th> International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: 香港(中華人民共和国)
  • Year and Date: 2010-05-31
• [Presentation] Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales. (2010)
  • Author(s): Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y
  • Organizer: Society of Environmental Toxicology and Chemistry.
  • Place of Presentation: Seville Spain
  • Year and Date: 2010-05-22
• [Presentation] Analysis of the tribu-tyltin-binding protein gene of Japanese flounder, Paralichthys olivaceus. (2010)
  • Author(s): Oshima, Y., Ooba Y., Satone H., Shimasaki, Y., Hashiguchi, Y.
  • Organizer: Society of Environmental Toxicology and Chemistry.
  • Place of Presentation: Seville Spain
  • Year and Date: 2010-05-22
• [Presentation] Tributyltin-binding protein type 1 rescued the inhibition of osteoblastic activity induced by TBT Poster Miki S., Ikeda K., Oba Y., Satone H., Shimasaki Y., Onikira N., Arakawa O, Oshima Y. Accumulation of tributyltin in the blood of pufferfish (2010)
  • Author(s): Satone H., Lee J.M., Kusakabe T., Kawabata, S., Suzuki N., Sasayama Y., Oba Y., Shimasaki Y., Oshima Y.
  • Organizer: Society of Environmental Toxicology and Chemistry
  • Place of Presentation: Sevillia Spain
• [Presentation] Synergistic toxicities of diazinon, fenitrothion and benthiocarb on Daphnia magna Poster (2010)
  • Author(s): Oshima, Y., Tanoue W., Kawaguchi, A., Yanagawa T., Shimasaki, Y.
  • Organizer: Society of Environmental Toxicology and Chemistry
  • Place of Presentation: Sevillia Spain
• [Presentation] Analysis of the tribu-tyltin-binding protein gene of Japanese flounder, Paralichthys olivaceus. POSTER (2010)
  • Author(s): Oshima, Y., Ooba Y., Satone H., Shimasaki, Y., Hashiguchi, Y.
  • Organizer: Society of Environmental Toxicology and Chemistry
  • Place of Presentation: Sevillia Spain
• [Presentation] GENE EXPRESSION PROFILE IN MEDAKA FISH EXPOSED TO TRICLOSAN, DICLOFENAC AND CARBAMAZEPINE POSTER (2010)
  • Author(s): M Nassef, Y K Unoki, Y Shimasaki, Y Oshima
  • Organizer: 第6回International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: 香港(中華人民共和国)
• [Presentation] INDUCTION OF THE TRIBUTYLTIN-BINDING PROTEIN 2 OF JAPANESE FLOUNDER, Paralichthys olivaceus BY EXPOSURE OF TRIBUTYLTIN-d27 Poster (2010)
  • Author(s): Y Oshima, T.Tawaratsumida, Y OBA, H SATONE, Y SHIMASAKI
  • Organizer: 第6回International Conference on Marine Pollution and Ecotoxicology
  • Place of Presentation: 香港(中華人民共和国)
• [Presentation] Toxic responses of embryos of Japanese medaka fish (Oryzias latipes) to pharmaceuticals and personal care products using in ovo nanoinjection Poster (2010)
  • Author(s): Nassef M, Seki M, Shimasaki Y1, Oshima Y
  • Organizer: Society of Environmental Toxicology and Chemistry Asia Pacific 2010
  • Place of Presentation: 広州(中華人民共和国)
• [Presentation] THE DETOXIFICATION AND EXCRETION FUNCTION OF TRIBUTYLTIN BINDING PROTEIN IN JAPANESE FLOUNDER, Paralichthyes olivaceus Platform. (2010)
  • Author(s): Oshima Y, Satone H, Yumi Oba Y, Lee JM, Kusakabe T, Kawabata S, Shimasaki Y, Suzuki N, Sasayama Y.
  • Organizer: Society of Environmental Toxicology and Chemistry Asia Pacific 2010
  • Place of Presentation: 広州(中華人民共和国)
• [Presentation] 魚類リポカリンは異物(トリブチルスズやフグ毒)と結合し体表粘液から排泄する機能を持つ (2010)
  • Author(s): 大嶋雄治
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学
• [Presentation] Expression of tributyltin-binding protein type 1 in silkworms using by a baculovirus gene expression system. (2009)
  • Author(s): Hina Satone, Jae Man Lee, Takahiro Kusakabe, Yumi Oba, Yohei Shimasaki, Yuji Oshima
  • Organizer: 14th International Symposium on Toxicity Assessment.
  • Place of Presentation: Mettz, France
  • Year and Date: 2009-08-30
• [Presentation] Evaluation of mixture toxicity and its mechanism on medaka(Oryzias latipes)embryos nanoinjected with a binary mixture of tributyltin and polychlorinated biphenyls (2009)
  • Author(s): Yuji Oshima, Shuhei Matsumoto, Sang Gyoon Kim, Kei Nakayama, Ik Joon Kang, Yohei Shimasaki, Tsuneo Honjo
  • Organizer: 30th Meeting of Society of Society of Environmental Toxicology and Chemistry
  • Place of Presentation: New Orlens, USA
• [Presentation] Tribu-tyltin-binding proteins from blood of Tiger puffer, Takifugu rubripes (2009)
  • Author(s): Yumi OBA, Akira YAMAUCHI, Yuji OSHIMA, Yohei SHIMASAKI, Hina SATONE, Takeshi KITANO, Miki NAKAO, Shun-ichiro KAWABATA4, Tsuneo HONJO
  • Organizer: 14th International Symposium on Toxicity Assessment
  • Place of Presentation: Mettz, France
• [Presentation] Expression of tributyltin-binding protein type 1 in silkworms using by a baculovirus gene expression system (2009)
  • Author(s): Hina Satone, Jae Man Lee, Takahiro Kusakabe, Yumi Oba, Yohei Shimasaki, Yuji Oshima
  • Organizer: 14th International Symposium on Toxicity Assessment
  • Place of Presentation: Mettz, France
• [Presentation] トリブチルスズ結合タンパク質とその遺伝子組換え体によるトリブチルスズーキンギョ鱗骨芽細胞 アルカリホスファターゼ活性阻害の回復 (2009)
  • Author(s): 大嶋雄治, 佐藤根妃奈, 大場由美, 李在萬, 日下部宜宏, 川畑俊一郎, 島崎洋平, 鈴木信雄, 笹山雄一
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学
• [Presentation] ヒラメトリブチルスズ結合タンパク質タイプ1はTBTと結合しその毒性を抑制する (2009)
  • Author(s): 大場由美, (九大院農)・鈴木信雄, (金沢大臨海)・島崎洋平・佐藤根妃奈, (九大院農)・笹山雄一, (金沢大臨海)・大嶋雄治, (九大院農)
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 盛岡市
• [Presentation] トリブチルスズ結合タンパク質(TBT-bp1)はTBTの骨芽細胞ALP活性阻害を回復させる (2009)
  • Author(s): 佐藤根妃奈, 李在萬, 日下部宜宏, 川畑俊一郎, 大場由美, 島崎洋平, 大嶋雄治, 鈴木信雄, 笹山雄一
  • Organizer: 第15回日本環境毒性学会・バイオアッセイ研究会合同研究発表会
  • Place of Presentation: 東京都
• [Remarks]
  • URL: http://bbs1.agr.kyushu-u.ac.jp/biosci-biotech/kankyo/publication.html
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/biosci-biotech/kankyo/index.html