Development of discrimination method between Dojou and Kara-dojou loaches using DNA markers for conservation of rice paddy ecosystem

• Project/Area Number: 20580270
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Irrigation, drainage and rural engineering/Rural planning
• Research Institution: National Agricultural Research Organization
• Principal Investigator: KOIZUMI Noriyuki National Agricultural Research Organization, 農村環境部・生態工学研究室, 主任研究員 (60301222)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ドジョウ / カラドジョウ / ミトコンドリアDNA / 核DNA / 判別式 / 生きもの調査 / Misgurnus anguillicaudatus / シトクロームb遺伝子 / 系統解析 / 種判別式 / 栃木県 / 水田生態系 / 農村環境 / 農業水路 / 集団遺伝解析

Research Abstract

Two loaches, the Dojou Misgurnus anguillicaudatus and Kara-dojou Paramisgurnus dabryanus inhabiting in rice paddies may be discriminated each other using mitochondrial and nuclear DNA markers in this study. Applying mitochondrial DNA analysis, the Dojou was separated into four genetic clades, Europe, China, Korea and Japan types. The Kara-dojou formed a single clade, Kara-dojou type. Results of nuclear DNA analysis showed the possibility of no hybridizing individual of them. To use a species discrimination equation with valuables of barbell length and body height could be available to decrease in a number of specimens used in the DNA analysis.

Research Products

• [Journal Article] 栃木県南東部におけるホトケドジョウのミトコンドリアDNAハプロタイプ (2010)
  • Author(s): 小出水規行,渡部恵司,高振麗, 他
  • Journal Title: 農業農村工学会論文集 265 Pages: 61-62
  • NAID: 10026349361
  • Peer Reviewed
• [Journal Article] 谷津田域におけるドジョウ集団の遺伝構造の解明-マイクロサテライトDNAを利用した千葉県下田川流域の事例- (2009)
  • Author(s): 小出水規行,竹村武士,森淳, 他
  • Journal Title: 農業農村工学会論文集 261 Pages: 21-29
  • Peer Reviewed
• [Journal Article] ミトコンドリアDNAによるドジョウの遺伝特性-チトクロームb遺伝子の塩基配列による系統解析- (2009)
  • Author(s): 小出水規行,竹村武士,渡部恵司, 他
  • Journal Title: 農業農村工学会論文集 259 Pages: 7-16
  • NAID: 10025640803
  • Peer Reviewed
• [Journal Article] Isolation and characterization of 19 polymorphic microsatellite DNA markers in the Japanese brown frog (Rana japonica) (2009)
  • Author(s): Koizumi N., Watabe K., Mori A., 他
  • Journal Title: Molecular Ecology Resources 9 Pages: 248-250
  • Peer Reviewed
• [Journal Article] マイクロサテライトDNAを用いた栃木県小貝川上流域のホトケドジョウ集団の予備遺伝解析 (2008)
  • Author(s): 小出水規行,渡部恵司,高振麗, 他
  • Journal Title: 農業農村工学会論文集 256 Pages: 55-61
  • NAID: 10025640504
  • Peer Reviewed
• [Journal Article] Reassessment of the taxonomy of Mesopodopsis orientalis (Tattersall, 1908) (Crustacea, Mysida) and proposal of a new species for the genus with an appendix on M. zeylanica Nouvel, 1954 (2008)
  • Author(s): Hanamura, Y.
  • Journal Title: Journal of Natural History 42 Pages: 2461-2500
  • Peer Reviewed
• [Journal Article] Occurrence of the non-indigenous freshwater prawn, Macrobrachium rosenbergii (De Man, 1879) (Decapoda, Caridea, Palaemonidae) in Madagasca (2008)
  • Author(s): Hanamura, Y.
  • Journal Title: Crustaceana 81 Pages: 1143-149
  • Peer Reviewed
• [Presentation] 栃木県におけるドジョウの遺伝的クレードの解明 (2010)
  • Author(s): 小出水規行,森淳,中茎元一, 他
  • Organizer: 平成22年度農業農村工学会大会講演会
  • Place of Presentation: 神戸大学
  • Year and Date: 2010-09-02
• [Presentation] 栃木県内のドジョウのDNA分析結果報告 (2010)
  • Author(s): 小出水規行
  • Organizer: 平成22年度メダカ里親の会総会
  • Place of Presentation: 宇都宮市平石市民センター
  • Year and Date: 2010-04-24
• [Presentation] ホトケドジョウの遺伝的多様性I-生息地内に存在する障壁物が遺伝的多様性に与える影響- (2010)
  • Author(s): 相木寛史・間野伸宏・小出水規行・小牧弘・廣瀬一美
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2010-03-28
• [Presentation] Introduction of genetic structure analysis for freshwater fish around paddy fields, Japan (2009)
  • Author(s): Koizumi, N.
  • Organizer: Seminar of USGS Fort Collins
  • Place of Presentation: USGS Fort Collins, USA
  • Year and Date: 2009-08-17
• [Presentation] Genetic structure analysis of populations of freshwater fish around paddy fields in Japan using microsatellite DNA : case study of Japanese endangered species (2009)
  • Author(s): Koizumi N., Watabe K., Mizutani M., 他
  • Organizer: the Japanese eight-barbel loach, Lefua echigonia, 33rd IAHR Congress
  • Place of Presentation: Vancouver
  • Year and Date: 2009-08-13
• [Presentation] Genetic structure analysis of populations of freshwater fish around paddy fields in Japan using microsatellite DNA : case study of Japanese endangered species, the Japanese eight-barbel loach, Lefua echigonia (2009)
  • Author(s): Koizumi, N., Watabe, K., Mizutani, M., Takemura, T., Mori, A.
  • Organizer: 33rd IAHR Congress
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2009-08-13
• [Presentation] マイクロサテライトDNAによる栃木県小貝川上流域におけるホトケドジョウ集団の遺伝構造解析 (2009)
  • Author(s): 小出水規行,渡部恵司,水谷正一, 他
  • Organizer: 平成21年度農業農村工学会大会講演会
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-08-06
• [Presentation] Introduction of genetic analysis for freshwater fish in paddy fields, Japan (2008)
  • Author(s): Koizumi, N.
  • Organizer: Joint Seminar on Fisheries Research Institute, Penang, Malaysia and Japan International Research Center for Agricultural Sciences, Japan
  • Place of Presentation: Fisheries Research Institute, Penang, Malaysia
  • Year and Date: 2008-11-20
• [Presentation] 小貝川上流域におけるホトケドジョウの遺伝的構造の解明:マイクロサテライトDNAマーカーを用いた予備解析 (2008)
  • Author(s): 小出水規行,渡部恵司,竹村武士, 他
  • Organizer: 平成20年度農業農村工学会大会講演会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2008-08-28
• [Book] 春の小川の淡水魚-その生息場と保全- (2009)
  • Author(s): 小出水規行
  • Publisher: 学報社