Development of discrimination method between Dojou and Kara-dojou loaches using DNA markers for conservation of rice paddy ecosystem
Project/Area Number |
20580270
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Irrigation, drainage and rural engineering/Rural planning
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Research Institution | National Agricultural Research Organization |
Principal Investigator |
KOIZUMI Noriyuki National Agricultural Research Organization, 農村環境部・生態工学研究室, 主任研究員 (60301222)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | ドジョウ / カラドジョウ / ミトコンドリアDNA / 核DNA / 判別式 / 生きもの調査 / Misgurnus anguillicaudatus / シトクロームb遺伝子 / 系統解析 / 種判別式 / 栃木県 / 水田生態系 / 農村環境 / 農業水路 / 集団遺伝解析 |
Research Abstract |
Two loaches, the Dojou Misgurnus anguillicaudatus and Kara-dojou Paramisgurnus dabryanus inhabiting in rice paddies may be discriminated each other using mitochondrial and nuclear DNA markers in this study. Applying mitochondrial DNA analysis, the Dojou was separated into four genetic clades, Europe, China, Korea and Japan types. The Kara-dojou formed a single clade, Kara-dojou type. Results of nuclear DNA analysis showed the possibility of no hybridizing individual of them. To use a species discrimination equation with valuables of barbell length and body height could be available to decrease in a number of specimens used in the DNA analysis.
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Report
(4 results)
Research Products
(17 results)
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[Presentation] Genetic structure analysis of populations of freshwater fish around paddy fields in Japan using microsatellite DNA : case study of Japanese endangered species, the Japanese eight-barbel loach, Lefua echigonia2009
Author(s)
Koizumi, N., Watabe, K., Mizutani, M., Takemura, T., Mori, A.
Organizer
33rd IAHR Congress
Place of Presentation
Vancouver, Canada
Year and Date
2009-08-13
Related Report
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