Research Project
Grant-in-Aid for Scientific Research (C)
Lb.reuteri generates 3-hydroxy propionaldehyde (3-HPA) as a substrate with glycerol. The dehydration type of 3-HPA is released from the cells and the hydration type or the dimer of 3-HPA with the reversibility (HPA system) in broth. All the types of 3-HPA are named generally with reuterin. There are no report that Lb. reuteri produced reuterin in animal gastrointestinal tract. Therefore, Lb.reuteri JCM 1112 associated gnotobiotic mouse was used, and 3-HPA in the mouse cecal contents was detected in vivo by the two-dimensional nuclear magnetic resonance (NMR) using ^<13>C_3-glycerol. The genes which associated with 3-HPA production from ^<13>C_3-glycerol was expressed in intentionality comparison with no glycerol intake group. Particularly, the expression of pduF which was a glycerol transporter gene recognized the significant difference with the group which take in no glycerol. here was no report that detected from probiotics such an antibacterial in vivo. It was proved that probiotics produced antibacterial in the gastrointestinal tract of the mammal according to our first report that in vivo detection of 3-HPA which was produced by Lb. reuteri.
All 2011 2010 2009 2008 Other
All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (12 results) Book (1 results) Remarks (1 results)
食品工業 54
Pages: 65-71
食品工業
Volume: 54 Pages: 65-71
J.Bacteriol. 191
Pages: 7630-7631
J.Dairy Sci. 92
Pages: 2400-2408
Anim.Sci.J. 80
Pages: 339-346
DNA Res. 15
Pages: 151-161
Lett. Appl. Microbiol. 46
Pages: 469-476
Pages: 375-386
