In vivo analysis of the gene expression of L.reuteri under the presence of glycerol
| Project/Area Number |
20580298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Azabu University |
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Principal Investigator |
MORITA Hidetoshi Azabu University, 獣医学部, 教授 (70257294)
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| Co-Investigator(Kenkyū-buntansha) |
KUWAHARA Tomomi 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (60263810)
TOH Hidehiro 独立行政法人理化学研究所, 基幹研究所, 基幹研究所研究員 (10353468)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Lactobacillus reuteri / ロイテリン / in vivo / 検出 / ノトバイオートマウス / グリセロール代謝 / in vivo検出 |
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| Research Abstract |
Lb.reuteri generates 3-hydroxy propionaldehyde (3-HPA) as a substrate with glycerol. The dehydration type of 3-HPA is released from the cells and the hydration type or the dimer of 3-HPA with the reversibility (HPA system) in broth. All the types of 3-HPA are named generally with reuterin. There are no report that Lb. reuteri produced reuterin in animal gastrointestinal tract. Therefore, Lb.reuteri JCM 1112 associated gnotobiotic mouse was used, and 3-HPA in the mouse cecal contents was detected in vivo by the two-dimensional nuclear magnetic resonance (NMR) using ^<13>C_3-glycerol. The genes which associated with 3-HPA production from ^<13>C_3-glycerol was expressed in intentionality comparison with no glycerol intake group. Particularly, the expression of pduF which was a glycerol transporter gene recognized the significant difference with the group which take in no glycerol. here was no report that detected from probiotics such an antibacterial in vivo. It was proved that probiotics produced antibacterial in the gastrointestinal tract of the mammal according to our first report that in vivo detection of 3-HPA which was produced by Lb. reuteri.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Complete genome sequence of probiotic Lactobacillus rhamnosus ATCC 531032009
Author(s)
Morita, H., Toh, H., Oshima, K., Murakami, M., Taylor, T.D., Igimi, S., Hattori, M.
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Journal Title
J.Bacteriol. 191
Pages: 7630-7631
Related Report
Peer Reviewed
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[Journal Article] Lactobacillus hayakitensis, L.equigenerosi and L.equi, predominant lactobacilli of the intestinal flora of healthy Thoroughbreds2009
Author(s)
Morita, H., Nakano, A., Shimazu, M., Toh, H., Nakajima, F., Nagayama, M., Hisamatsu, S., Kato, Y., Takagi, M., Takami, H., Akita, H., Matsumoto, M., Masaoka, T., Murakami, M.
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Journal Title
Anim.Sci.J. 80
Pages: 339-346
Related Report
Peer Reviewed
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[Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island forreuterin and cobalamin production2008
Author(s)
Morita, Toh, Fukuda, Horikawa, Oshima, Suzuki, Murakami, Hisamatsu, Kato, Takizawa, Fukuoka, Yoshimura, Itoh, 0' Sullivan, McKay, Ohno, K ikuchi, Masaoka andHattori
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Journal Title
DNA Res. 15
Pages: 151-161
Related Report
Peer Reviewed
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[Journal Article] Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isol ated from a healthy adult2008
Author(s)
Oshima, Toh, Ogura, Sasamoto, Morita, Park, Ooka, Iyoda, Taylor, Hayashi, Itoh, Hattori
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Journal Title
DNA Res. 15
Pages: 375-386
Related Report
Peer Reviewed
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