| Project/Area Number |
20580302
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
SATO Kan Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (20250730)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 代謝 / 内分泌制御 / 肝臓 / 脂質シグナル / apolipoprotein B / 中鎖脂肪酸 / 転写調節 / HNF4-α / apolipoproteinB |
|---|
| Research Abstract |
Apolipoprotein B (apoB) is an integral protein component of very low density lipoprotein (VLDL), a key regulator of fat mass in farm animals. ApoB mRNA expression and apoB protein synthesis in hepatocytes are relatively constitutive and generally does not change under conditions that alter apoB secretion. Therefore, VLDL assembly is regulated largely at co- and post-translational levels. In present study, we investigated the identification and characterization of novel lipid signaling pathway to impair the apoB mRNA synthesis. ApoB mRNA expression in chickens supplemented with octanoate (C8:0)- and decanoate (C10:0)-triacylglycerols in diets significantly decreased compared to that in control group. This result suggested that the impairment of apoB mRNA expression by medium chain fatty acids (MCFAs) in chicken causes in normal and physiological condition in chickens. The two MCFAs responsible elements were determined in chicken apoB promoter, which was cited in -200 and -1000～-2000bp from coding region. These results clearly show a novel lipid signaling pathway, which is associated by MCFAs, and the signaling impaired apoB mRNA expression in chicken hepatocytes.
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