Establishment of Detection Method of Endocrine Disrupter by Avian Yolk-Related Gene Expression

• Project/Area Number: 20580307
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Shizuoka University
• Principal Investigator: MORI Makoto Shizuoka University, 農学部, 教授 (90143411)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 代謝 / 内分泌制御 / 畜産学 / 内分泌 / 発生・分化 / 家禽 / 内分泌撹乱 / ウズラ / ホルモン / 肝臓 / 卵黄

Research Abstract

A sensitive method by using the gene expression related to avian yolk protein was established to detect the effect of endocrine disrupters. Especially focusing on oogenesis and embryonic growth, the most appropriate gene is suggested to be quail very-low-density-lipoprotein. The effect of heavy metals and antioxidants as well as diethylstilbestrol was examined with the causal relationship with dose-related manner at molecular level.

Research Products

• [Journal Article] The Biological Monitoring of Wild Birds - Part 2 : The Possibility of a New Index for Biological Monitoring. (2010)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Journal Title: International Journal of Energy, Environment, and Economics 19 Pages: 145-163
  • Peer Reviewed
• [Journal Article] The Biological Monitoring of Wild Birds - Part 1 : The Cadmium Content of Organs from Migratory Birds. (2010)
  • Author(s): Mariko Mochizuki, Mayumi Shiozawa, Makoto Mori, Hiroshi Kajigaya, Shin-ichi Hayama, Yoshitsugu Ochiai, Ryo Hondo, Fukiko Ueda
  • Journal Title: International Journal of Energy, Environment, and Economics 19 Pages: 22-23
  • Peer Reviewed
• [Journal Article] A New Technique for Biological Monitoring Using Wildlife. (2010)
  • Author(s): Mariko Mochizuki, Makoto Mori, Mutsumi Miura, Ryo Hondo, Takashi Ogawa, Fukiko Ueda
  • Journal Title: International Journal of Energy, Environment, and Economics 18 Pages: 88-94
  • Peer Reviewed
• [Journal Article] Hepatoprotective Effects of Flavonoids from Shekwasha (Citrus depressa) against D-Galactosamine-induced Liver Injury in Rats. (2010)
  • Author(s): Toshiyuki Akachi, Yasuyuki Shiina, Yayoi Ohishi, Takumi Kawaguchi, Hirokazu Kawagishi, Tatsuya Morita, Makoto Mori, Kimio Sugiyama
  • Journal Title: Journal of Nutritional Science and Vitaminology 56 Pages: 60-67
  • NAID: 10025972955
  • Peer Reviewed
• [Journal Article] Effect of Selenium on Hatchability and Cellular Glutathione Peroxidase mRNA Expression during Embryogenesis in Japanese Quail (Coturnix japonica). (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 340-344
  • NAID: 10025549635
  • Peer Reviewed
• [Journal Article] In-ovo Cadmium Toxicity in Developing Quail Embryo (Coturnix japonica) : Sex-dependent Responses to Ascorbic Acid Protection. (2009)
  • Author(s): Md.Shahidur Rahman, Mariko Mochizuki, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 334-339
  • NAID: 10025549597
  • Peer Reviewed
• [Journal Article] Hepatic Cystathionine β-Synthase Activity Does Not Increase in Response to Methionine Supplementation in Rats Fed a Low Casein Diet : Association with Plasma Homocysteine Concentrations. (2009)
  • Author(s): Seiya Ohuchi, Tatsuya Morita, Makoto Mori, Kimio Sugiyama
  • Journal Title: Journal of Nutritional Science and Vitaminology 55 Pages: 178-185
  • NAID: 10025970986
  • Peer Reviewed
• [Journal Article] Cloning and Expression of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica). (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 52-58
  • NAID: 10024799012
  • Peer Reviewed
• [Journal Article] Cloning and Expression of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica) (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 52-58
  • NAID: 10024799012
  • Peer Reviewed
• [Journal Article] In-ovo Cadmium Toxicity in Developing Quail Embryo (Coturnix japonica) : Sex-dependent Responses to Ascorbic Acid Protection (2009)
  • Author(s): Md.Shahidur Rahman, Mariko Mochizuki, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 334-339
  • NAID: 10025549597
  • Peer Reviewed
• [Journal Article] Effect of Selenium on Hatchability and Cellular Glutathione Peroxidase mRNA Expression during Embryogenesis in Japanese Quail (Coturnix japonica) (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 340-344
  • NAID: 10025549635
  • Peer Reviewed
• [Journal Article] Cloning and Express ion of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica). (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Journal Title: Journal of Poultry Science 46 Pages: 52-58
  • Peer Reviewed
• [Journal Article] Absence of Donor-Derived Zona Pellucida Protein C Homologue in Inner Perivitelline Layer of Peking Duck (Anas platyrhynchos) - Japanese Quail (Coturnix coturnix japonica) Chimeras (Duails). (2008)
  • Author(s): J.Sha, J.Gao, J.Li, Q.Zhao, G.Tao, C.Zhao, H.Han, M.Mori, Z.Li
  • Journal Title: Poultry Science 87 Pages: 2064-2072
  • Peer Reviewed
• [Journal Article] Cadmium Disrupts the Diethylstilbestroi Effect on Very-Low-Density Apolipoprotein II Gene Transcription in the Liver of Japanese Quail (Coturnix japonica). (2008)
  • Author(s): M. Shahidur Rahman, Mariko Mochizuki, Makoto Mori
  • Journal Title: Journal of Poultry Science 45 Pages: 62-66
  • Peer Reviewed
• [Journal Article] A New Index for Evaluation of Cadmium Pollution in Birds and Mammals. (2008)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Journal Title: Environmental Monitoring and Assessment 137 Pages: 35-49
  • Peer Reviewed
• [Presentation] A New Index for Heavy Metals in Biological Monitoring. (2011)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Organizer: 9th Asia Pacific Poultry Conference
  • Place of Presentation: Taipei
  • Year and Date: 2011-03-22
• [Presentation] A New Index for Heavy Metals in Biological Monitoring. (2011)
  • Author(s): Mariko Mochizuki, Makoto Mori, RyoHondo, Fukiko Ueda
  • Organizer: 9th Asia Pacific Poultry Conference
  • Place of Presentation: 台湾国際会議場(台北市)
  • Year and Date: 2011-03-21
• [Presentation] 野鳥の重金属汚染に関する疫学的研究5)新たな指標による野鳥の鉛汚染の解析 (2010)
  • Author(s): 竹本麻里子・森誠・奥富幸・羽山伸一・梶ヶ谷博・本藤良・植田富貴子・望月眞理子
  • Organizer: 第35回鳥類内分泌研究会
  • Place of Presentation: 岡山三光荘
  • Year and Date: 2010-11-25
• [Presentation] 野鳥の重金属汚染に関する疫学的研究6)因子分析による複合汚染の解析 (2010)
  • Author(s): 貝塚千博・森誠・本藤良・植田富貴子・望月眞理子
  • Organizer: 第35回鳥類内分泌研究会
  • Place of Presentation: 岡山三光荘
  • Year and Date: 2010-11-25
• [Presentation] Potential Actions of Relaxin and Its Related Peptide on Male Reproductive Processes in Domestic Animals. (2010)
  • Author(s): Tetsuya Kohsaka, Keiichiro Yogo, Makoto Mori
  • Organizer: 14th AAAP Animal Science
  • Place of Presentation: Congress Pingtung
  • Year and Date: 2010-08-25
• [Presentation] A New Index for Heavy Metals in Biological Monitoring. (2009)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Organizer: 5th International Conference on Energy, Environment, Ecosystems and Sustainable
  • Place of Presentation: Development Athens
  • Year and Date: 2009-09-30
• [Presentation] Acute Effect of Cadmium on Female Reproduction in Birds. (2009)
  • Author(s): Makoto Mori, Md. Shahidur Rahman, Mariko Mochizuki, Ryo Hondo, Fukiko Ueda
  • Organizer: 5th International Conference on Energy, Environment, Ecosystems and Sustainable Development
  • Place of Presentation: Athens
  • Year and Date: 2009-09-30
• [Presentation] A New Technique for Biological Monitoring. (2009)
  • Author(s): 植田富貴子, 望月眞理子, 本藤良, Ryo Hondo
  • Organizer: 5th International Conference on Energy, Environment, Ecosystems and Sustainable Development
  • Place of Presentation: Athens
  • Year and Date: 2009-09-29
• [Presentation] Acute Effect of Cadmium on Female Reproduction in Birds (2009)
  • Author(s): Makoto Mori, Md.Shahidur Rahman, Mariko Mochizuki, Ryo Hondo, Fukik Ueda
  • Organizer: 5th International Conference on Energy, Environment, Ecosystems and Sustainable Development
  • Place of Presentation: アテネ (ギリシャ)
  • Year and Date: 2009-09-29
• [Presentation] Basic Investigation for an Epidemiological Study on Cadmium Contamination of Wildlife - Cadmium Distribution in the Rat Body after Intravenous Cadmium Exposure. (2009)
  • Author(s): Fukiko Ueda, Makoto Mori, Takashi Takano, Yoshitsugu Ochiai, Ryo Hondo, Mariko Mochizuki
  • Organizer: 5th International Conference on Energy, Environment, Ecosystems and Sustainable
  • Place of Presentation: Development Athens
  • Year and Date: 2009-09-28
• [Presentation] 野鳥の重金属汚染に関する疫学調査 -9) カドミウム汚染の性差による違い (2009)
  • Author(s): 奥富幸・森誠・梶ヶ谷博・落合由嗣・本藤良・小河孝・植田富貴子・望月眞理子
  • Organizer: 第147回日本獣医学会学術集会 公衆衛生学分科会
  • Place of Presentation: 栃木県総合文化センター
  • Year and Date: 2009-04-02
• [Presentation] Effect on Selenium on Hatchability and Glutathione Peroxidase mRNA Expression during Embryogenesis in Japansese Quail. (2009)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Organizer: 日本家禽学会 春季大会 日本大学 生物資源科学部
  • Place of Presentation: 湘南キャンパス
  • Year and Date: 2009-03-28
• [Presentation] 野鳥の重金属汚染に関する疫学的研究3)新たな指標であるカドミウム標準回帰直線を用いた生物モニタリングの可能性 (2008)
  • Author(s): 高梨ありこ・森誠・小河孝・本藤良・植田富貴子・望月眞理子
  • Organizer: 第33回鳥類内分泌研究会
  • Place of Presentation: 草津温泉中沢ヴィレッジ
  • Year and Date: 2008-11-13
• [Presentation] 野鳥の重金属汚染に関する疫学的研究4)カドミウム標準回帰直線を用いた実例 (2008)
  • Author(s): 三浦睦・森誠・小河孝・本藤 良・植田富貴子・望月眞理子
  • Organizer: 第33回鳥類内分泌研究会
  • Place of Presentation: 草津温泉中沢ヴィレッジ
  • Year and Date: 2008-11-13
• [Presentation] Effect of Selenium on Cellular Glutathione Peroxidase mRNA Expression and Consequent Hatchability in Japanese Quail (Coturnix japonica). (2008)
  • Author(s): Sopon Wilaison, Makoto Mori
  • Organizer: 13th AAAP Animal Science Congress Hanoi
  • Year and Date: 2008-09-25
• [Presentation] Identification of a 35 kDa Glycoprotein, Zona Pellucida Protein C (ZPC) Homologue in Inner Perivitelline Layer Produced by Peking Duck-Japanese Quail Chimaeras. (2008)
  • Author(s): Zandong Li, Jin Sha, Junshuang Gao, Makoto Mori
  • Organizer: 日本家禽学会 秋季大会
  • Place of Presentation: 北里大学 獣医学部
  • Year and Date: 2008-08-29
• [Presentation] Ascorbic Acid Protection of Yolk Protein Gene Suppression by Cadmium in Japanese Quail (Coturnix japonica). (2008)
  • Author(s): Md Shahidur Rahman, Makoto Mori
  • Organizer: 5th SETAC (The Society of Environmental Toxicology and Chemistry)
  • Place of Presentation: World Congress Sydney
  • Year and Date: 2008-08-06
• [Presentation] Suppression of Yolk Protein Gene Expression and Egg Production by Cadmium in Japanese Quail (Coturnix japonica) : Protection by Ascorbic Acid. (2008)
  • Author(s): Md Shahidur Rahman, Makoto Mori
  • Organizer: 23rd World's Poultry Congress 2008
  • Place of Presentation: Brisbane
  • Year and Date: 2008-07-02
• [Presentation] Suppression of Yolk Protein Gene Expression and Egg Production by Cadmium in Japanese Quail (Coturnix japonica) : Protection by Ascorbic Acid. (2008)
  • Author(s): Md Shahidur Rahman, Makoto Mori
  • Organizer: World Poultry Congress
  • Place of Presentation: ブリスペン(オーストラリア)
• [Book] Impact, Monitoring and Management of Environmental Pollution(Chapter 15. A Cadmium Standard Regression Line : A Possible New Index for Biological Monitoring.)(Ed.A.El Numr) (2010)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Publisher: Nova Science Publishers : New York
• [Book] Wildlife : Destruction, Conservation and Biodiversity(Biological Monitoring Using A New Technique.)(Ed.J.D.Harris, P.L.Brown) (2009)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Publisher: Nova Science Publishers : New York
• [Book] The Biological Monitoring of Wild Birds ; The Cadmium Content of Organs from Migratory Birds. (2009)
  • Author(s): Mariko Mochizuki, Makoto Mori, Hiroshi Kajigaya, Shin-ichi Hayama, Yoshitsugu Ochiai, Ryo Hondo, Fukiko Ueda
  • Publisher: Biological Monitoring
• [Book] Wildlife : Destruction, Conservation and Biodiversity (2009)
  • Author(s): Mariko Mochizuki, Makoto Mori, Ryo Hondo, Fukiko Ueda
  • Total Pages: 359
  • Publisher: Nova Science Publishers : New York