The structure of relaxin-like peptide expressed in the boar testis and the implication in testicular function via its receptor LGR8
Project/Area Number |
20580308
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
|
Research Institution | Shizuoka University |
Principal Investigator |
KOHSAKA Tetsuya Shizuoka University, 農学部, 教授 (10186611)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | relaxin / RLF / LGR / testis / リラキシン / 関連因子 / 精巣 / 受容体 / 局在 / 精子形成 / 生理活性 / 発現 / 構造解析 / プロセシング |
Research Abstract |
This study has purified native relaxin-like peptide (RLF) from boar testes, and demonstrated for the first time at nay species that native RLF is secreted from the Leydig cells as a 12-kDa heterotrimeric proform structure consisting of an A-B-C-domain with site-specific disulphide bonds and full biological activity. RLF secreted from Leydig cells was released not only into the blood circulation, but also into the interstitial compartment and is transported into the seminiferous compartments, where its own receptor LGR8 was expressed, suggesting that RLF may function as a paracrine and an autocrine and/or endocrine factor on seminiferous germ cells and Leydig cells, respectively.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Evidence for expression of relaxin hormone-receptor system in the boar testis.2010
Author(s)
Kato S, Siqin, Minagawa I, Aoshima T, Sagata D, Konishi H, Yogo K, Kawarasaki T, Sasada H, Tomogane H, Kohsaka T.
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Journal Title
J.Endocrinol. 207
Pages: 135-149
Related Report
Peer Reviewed
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[Journal Article] Partial cDNA sequence of a relaxin-like factor (RLF) receptor, LGR8 and possible existence of the RLF ligand-receptor system in goat testes.2010
Author(s)
Siqin, Nakai M, Hagi T, Kato S, Pitia AM, M.Kotani, Y.Odanaka, Y.Sugawara, K.Hamano, K.Yogo, Y.Nagura, M.Fujita, H.Ssasada, E.Sato, Kohsaka T.
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Journal Title
Anim.Sci.J. 81
Pages: 681-686
Related Report
Peer Reviewed
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[Journal Article] Zona pellucida protein ZP2 is expressed in the oocyte of Japanese quail (Coturnix japonica).2010
Author(s)
Kinoshita M, Rodler D, Sugiura K, Matsushima K, Kansaku N, Tahara K, Tsukada A, Ono H, Yoshimura T, Yoshizaki N, Tanaka R, Kohsaka T, Sasanami T.
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Journal Title
Reproduction 139
Pages: 359-371
Related Report
Peer Reviewed
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