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Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.

Research Project

Project/Area Number 20580311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKagoshima University

Principal Investigator

YOSHIDA Mitsutoshi  鹿児島大学, 農学部, 教授 (00174954)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Kazuchika  鹿児島大学, 農学部, 准教授 (70363611)
SATO Masahiro  鹿児島大学, フロンティアサイエンス研究推進センター, 教授 (30287099)
Research Collaborator HIMAKI Takehiro  鹿児島大学, 大学院・連合農学研究科, 学生
MORI Hironori  鹿児島大学, 大学院・農学研究科, 学生
TSUDA Kennichirou  鹿児島大学, 大学院・農学研究科, 学生
MIZOBE Yamato  鹿児島大学, 大学院・農学研究科, 学生
SUETOMO Masashi  鹿児島大学, 大学院・農学研究科, 学生
OGAWA Jyunki  鹿児島大学, 大学院・農学研究科, 学生
AKAHOSHI Kaoru  鹿児島大学, 農学部, 学生
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords体細胞クローン / 遺伝子改変 / 脱アセチル化 / トリコスタチンA / スベロイルビスヒドロキサム酸 / スクリパタイド / ラトランキュリンA / クラウン系ミニブタ / 発生 / バルプロ酸
Research Abstract

The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors(HADCi) and an actin polymerisation inhibitor, latrunculin A(LatA) on in vitro and in vivo development of somatic cell nuclear transfer(SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher(p<0. 05) in SCNT embryos treated with HADCi and 0. 5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (11 results)

  • [Journal Article] Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010

    • Author(s)
      Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
    • Journal Title

      Cell Reprogram

      Volume: Vol.12 Pages: 67-74

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Latrunculin a dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells2010

    • Author(s)
      Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M.
    • Journal Title

      Cell Reprogram

      Volume: Vol.12 Pages: 127-131

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010

    • Author(s)
      Himaki T, Yokomine TA, Sato M, Takao S, Miyoshi K, Yoshida M.
    • Journal Title

      Anim Sci J

      Volume: Vol.81

    • NAID

      10027889588

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results toward production of xenograft-competent miniature pigs2010

    • Author(s)
      Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M
    • Journal Title

      J Reprod Dev

      Volume: Vol.56 Pages: 630-638

    • NAID

      10027730311

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010

    • Author(s)
      Himaki T, Yokomine TA, Sato M, Takao S, Miyoshi K, Yoshida M
    • Journal Title

      Anim Sci J

      Volume: 81 Pages: 558-563

    • NAID

      10027889588

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Latrunculin a dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells2010

    • Author(s)
      Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M
    • Journal Title

      Cell Reprogram

      Volume: 12 Pages: 127-131

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results to ward production of xenograft-competent miniature pigs2010

    • Author(s)
      Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M
    • Journal Title

      J Reprod Dev

      Volume: 56 Pages: 630-638

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Valpronic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010

    • Author(s)
      Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
    • Journal Title

      Cellular Reprogramming 12

      Pages: 67-74

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009

    • Author(s)
      Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
    • Journal Title

      J Reprod Dev

      Volume: Vol.55 Pages: 661-669

    • NAID

      10026322831

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] プロスタグランジンF2α類縁物質と性腺刺激ホルモン投与によるクラウン系雌ミニブタの発情同期化に関する研究2009

    • Author(s)
      日巻武裕, 梅野知子, 三好和睦, 吉田光敏
    • Journal Title

      鹿児島県獣医師会会報

      Volume: Vol.23 Pages: 5-7

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009

    • Author(s)
      Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M
    • Journal Title

      J Reprod Dev 55

      Pages: 661-669

    • NAID

      10026322831

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of demecolcine and sucrose on the incidence of cytoplasmicprotrusions containing chromosomes in pig oocytes matured in vitro.2008

    • Author(s)
      Miyoshi K, Mori H, Yamamoto H, Kishimoto M, Yoshida M
    • Journal Title

      J Reprod Dev 54

      Pages: 117-121

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of cycloheximide on parthenogenetic development of pig oocytesactivated by ultrasound treatment.2008

    • Author(s)
      Mori H, Mizobe Y, Inoue S, Uenohara A, Takeda M, Yoshida M, Miyoshi K
    • Journal Title

      J Reprod Dev 54

      Pages: 364-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 活性化後のスクリプタイドまたはスベロイルビスヒドロキサム酸処理がミニブタ体細胞クローン胚の体外発生に及ぼす影響2011

    • Author(s)
      小川純輝、小澤政之、三好和睦、吉田光敏
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      岩手
    • Year and Date
      2011-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] マイクロアレイを用いたブタ体外成熟卵子の網羅的遺伝子発現解析2010

    • Author(s)
      脇詩織、三好和睦、吉田光敏
    • Organizer
      第112回日本畜産学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 活性化後のラトランキュリンA処理がミニブタ成体腎細胞に由来するクローン胚の発生能に及ぼす影響2010

    • Author(s)
      日巻武裕、溝部大和、津田健一郎、末友雅士、三好和睦、吉田光敏
    • Organizer
      第112回日本畜産学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 活性化後のラトランキュリンA処理がミニブタ成体腎細胞に由来するクローン胚の発生能に及ぼす影響2010

    • Author(s)
      日巻武裕, 溝部大和, 津田健一郎, 末友雅士, 三好和睦, 吉田光敏
    • Organizer
      日本畜産学会第112回大会
    • Place of Presentation
      明治大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Latrunculin A dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified Clawn miniature pig cells2009

    • Author(s)
      Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M.
    • Organizer
      42nd Meeting of the Society for the Study of Reproduction
    • Place of Presentation
      アメリカ合衆国
    • Year and Date
      2009-07-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Latrunculin A dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified Clawn miniature pig cells2009

    • Author(s)
      Himaki T, Mori H, Mizobe Y, Miyosi K, Sato M, Takao S, Yoshida M
    • Organizer
      米国繁殖学会第42回大会
    • Place of Presentation
      Pittsburgh, USA
    • Year and Date
      2009-07-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effect of latrunculin A on developmental potentials of electrically activated porcine oocytes2009

    • Author(s)
      王建輝、池海英、三好和睦、吉田光敏
    • Organizer
      第110回日本畜産学会
    • Place of Presentation
      神奈川
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Effect of Latrunculin A on Developmental Potentials of Electrically Activated Porcine Oocvtes2009

    • Author(s)
      王 建輝, 池 海英, 三好和睦, 吉田光敏
    • Organizer
      日本畜産学会第110回大会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] α-1, 3-galactosiyl-transferase遺伝子ノックアウトベクターを挿入したクラウン系ミニブタ体細胞に由来するクローン胚の体内発生能の検討2009

    • Author(s)
      日巻武裕、森寛倫、溝部大和、三好和睦、佐藤正宏、高尾尊身、吉田光敏
    • Organizer
      第12回日本異種移植研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      2009-03-07
    • Related Report
      2010 Final Research Report
  • [Presentation] バルプロ酸がミニブタ体細胞クローン胚の体外発生に及ぼす影響2009

    • Author(s)
      森寛倫、溝部大和、赤坂恵理、小澤明央、吉田光敏、佐藤正宏、三好和睦
    • Organizer
      第12回日本異種植研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      2009-03-07
    • Related Report
      2010 Final Research Report
  • [Presentation] バルプロ酸がミニブク体細胞クローン胚の体外発生に及ぼす影響2009

    • Author(s)
      森寛倫, 溝部大和, 赤坂恵理, 小澤明央, 吉田光敏, 佐藤正宏, 三好和睦
    • Organizer
      第12回日本異種移植研究会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2009-03-07
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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