| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
The technical establishment of boar sperm cryopreservation isindispensable for effective breeding of the scarce Okinawan native Agu pig. In this study, the addition of ascorbic acid 2-Ο-α-glucoside(AA-2G) and low-density lipoprotein(LDL) tothe freezing extender reduced the plasma membrane damage in post-thaw Agu sperm as aconsequence of the protection of sperm against extracellular oxidative stress and cold shockdamage, respectively, during cryopreservation. Moreover, treatment with an artificial anti-cell death protein(PTD-FNK protein) prevented the mitochondrial dysfunction leading to the apoptotic-cell death during cryopreservation. Therefore, these results indicate that the post-thaw qualities of fragile Agu sperm is effectively improved throughthe established techniques in this study.
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