Unraveling the molecular mechanisms of spermatogenesis using differentiation-deficient spermatogonial stem cells
| Project/Area Number |
20580313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kitasato University |
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Principal Investigator |
KUBOTA Hiroshi Kitasato University, 獣医学部, 准教授 (80263094)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIUCHI Kazue 北里大学, 獣医学部, 助教 (90509184)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 生殖幹細胞 / 精原幹細胞 / 精子形成 / 増殖因子 |
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| Research Abstract |
The KIT receptor tyrosine kinase is required for normal spermatogenesis. Although spermatogenesis is abolished in the testis of W^v/W^v mice due to the Kit gene mutation, undifferentiated spermatogonia with spermatogonial stem cell (SSC) phenotype were identified. The W^v/W^v spermatogonia were self-renewed in a modified SSC culture system, indicating they were SSCs. Comparison of the gene expression profiles of the W^v/W^v and wild-type SSCs identified several genes that were expressed differentially in the two SSC populations. These genes might play a role in spermatogonial differentiation.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Hepatic stem cells and hepatoblasts : identification isolation, and ex vivo maintenance.2008
Author(s)
Wauthier E, Schmelzer E, Turner W, Zhang L, Lecluyse E, Ruiz J, Turner R, Furth ME, Kubota H, Lozoya O, Barbier C, McClelland R, Yao HL, Moss N, Bruce A, Ludlow J, Reid LM
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Journal Title
Methods in Cell Biology vol.86
Pages: 137-225
Related Report
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