Development of the safe and functional avirulent neurotracing virus producing system.

Research Project

Project/Area Number	20580319
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	Tokyo University of Agriculture and Technology
Principal Investigator	TANIGUCHI Takahide Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (70282803)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	ウイルス / 脳・神経 / バイオテクノロジー / ニューロトレーサー
Research Abstract	The entire genome sequence of the porcine hamagglutinating encephalomyelitis virus (PHEV) 67N strain, neurotropic coronavirus, was determined, and the sequence data is useful for reverse genetics of PHEV to develop the PHEV based neurotracing virus. There is about 400 base insertion in ns2 gene of neurotropic 67N strain and 200 base deletion in ns2 gene of non neurotropic OSN204 strain. The results of in vitro PHEV infection to Neuroblastoma and glioma cell line indicated that PHEV 67N strain have high neurotropism.

Report