Role of the TGF-β family and Mitf during osteoclastogenesis

• Project/Area Number: 20580330
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Azabu University
• Principal Investigator: MURAKAMI Masaru Azabu University, 獣医学部, 教授 (80271360)
• Co-Investigator(Renkei-kenkyūsha): FUNABA Masayuki 京都大学, 農学研究科, 准教授 (40238655) ; NISHINO Yoshii 京都産業大学, 総合生命科学部, 准教授 (00271544)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: TGF-βファミリー / Mitf / 遺伝子発現制御 / 破骨細胞分化 / シグナル伝達

Research Abstract

We explored the role of TGF-β family that regulates a broad range of biological processes and Mitf that is a tissue-specific transcription factor, in the process of RANKL-induced osteoclast formation. This study indicates that Mitf-E plays a crucial role in osteoclastogenesis, and that the TGF-β family positively regulates the expression of Mitf-E as well as osteoclast marker genes. Stimulatory activity of the TGF-β family on osteoclastogenesis may be partly elicited through up-regulation of Mitf-E.

Research Products

• [Journal Article] Role of endogenous TGF-β family in myogenic differentiation of C2C12 cells. (2011)
  • Author(s): Furutani Y., Umemoto T., Murakami M., Matsui T., Funaba M.
  • Journal Title: J.Cell.Biochem. 112(2) Pages: 614-624
  • NAID: 120005593583
• [Journal Article] Regulatory expression of genes related to metastasis by TGF-β and activin A in B16 murine melanoma cells. (2010)
  • Author(s): Murakami M., Suzuki M., Nishino Y., Funaba M.
  • Journal Title: Mol.Biol.Rep. 37 Pages: 1279-1286
• [Journal Article] Regulatory expression of genes related to metastasis by TGF-β and activin A in B16 murine melanoma cells. (2010)
  • Author(s): Murakami M., Suzuki M., Nishino Y., Funaba M.
  • Journal Title: Mol.Biol.Rep. Volume: 37 Pages: 1279-1286
  • Peer Reviewed
• [Journal Article] Role of endogenous TGF-β family in myogenic differentiation of C2C12 cells. (2010)
  • Author(s): Furutani Y., Umemoto T., Murakami M., Matsui T., Funaba M.
  • Journal Title: J.Cell.Biochem. Volume: 112(2) Pages: 614-624
  • NAID: 120005593583
  • Peer Reviewed
• [Journal Article] Differential responses to oxidative stress and calcium influx on expression of the transforming growth factor-β family in myoblasts and myotubes. (2009)
  • Author(s): Furutani Y., Murakami M., Funaba M.
  • Journal Title: Cell Biochem.Funct. 27(8) Pages: 578-582
• [Journal Article] Receptor expression modulates the specificity of transforming growth factor-β signaling pathways. (2009)
  • Author(s): Murakami M., Kawachi H., Ogawa. Nishino Y., Funaba M.
  • Journal Title: Genes Cells 14(4) Pages: 469-482
• [Journal Article] Differential responses to oxidative stress and calcium influx on expression of the transforming growth factor-β family in myoblasts and myotubes. (2009)
  • Author(s): Furutani Y., Murakami M., Funaba M.
  • Journal Title: Cell Biochem. Funct. 27 Pages: 578-582
  • Peer Reviewed
• [Journal Article] Receptor expression modulates the specificity of transforming growth factor-β signaling pathways. (2009)
  • Author(s): Murakami M. . Kawachi H., Ogawa K., Nishino Y., Funaba M.
  • Journal Title: Genes Cells. 14 Pages: 469-482
  • Peer Reviewed
• [Journal Article] Regulatory expression of genes related to. metastasis by TGF-β and activin A in B16 murine melanoma cells. (2009)
  • Author(s): Murakami M., Suzuki M., Nishino Y., Funaba M.
  • Journal Title: Mol. Biol. Rep. (in press)(Epub ahead of print)
  • Peer Reviewed
• [Presentation] RANKL刺激による破骨細胞分化過程におけるMitf-EとActivinの役割 (2010)
  • Author(s): 浅井久美子, 舟場正幸, 村上賢
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] MitfはC2C12細胞の筋分化において細胞融合を促進する (2010)
  • Author(s): 大石亮, 舟場正幸, 村上賢
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] 筋分化における内因性BMP4の役割 (2010)
  • Author(s): 梅本岳尚,村上賢,舟場正幸,松井徹
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
• [Presentation] RANKL刺激による破骨細胞分化過程におけるMitf-EとActivinの役割 (2010)
  • Author(s): 浅井久美子,舟場正幸,村上賢
  • Organizer: 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
• [Presentation] MitfはC2C12細胞の筋分化において細胞融合を促進する (2010)
  • Author(s): 大石亮,舟場正幸,村上賢
  • Organizer: 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
• [Presentation] Expression of Mitf isoforms in mouse skeletal muscle and during myogenesis. (2009)
  • Author(s): 大石亮、舟場正幸、村上賢
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
• [Presentation] The role of TGF-β family in myogenic differentiation in C2C12 cells. (2009)
  • Author(s): 古谷勇馬,村上賢,舟場正幸,松井徹
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
• [Presentation] Expression of Mitf isoforms in mouse skeletal muscle and during myogenesis (2009)
  • Author(s): 大石亮、舟場正幸、村上賢
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
• [Presentation] The role of TGF-β family in myogenic differentiation in C2C12 cells (2009)
  • Author(s): 古谷勇馬, 村上賢, 舟場正幸, 松井徹
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
• [Presentation] マウス臓器におけるTgf-βファミリー受容体の発現 (2008)
  • Author(s): 大石亮、舟場正幸、村上賢
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] TGF-βによるBMP-regulated Smadのリン酸化とBMPによるTGF-β/activin-regulated Smadのリン酸化 (2008)
  • Author(s): 村上賢、小川健司、舟場正幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] Modulation of signal specificity of the TGF-β family related to the receptor expression. (2008)
  • Author(s): Funaba M., Murakami M., Ogawa K.
  • Organizer: 48th The American Society for Cell Biology Annual Meeting.
• [Presentation] マウス臓器におけるTgf-βファミリー受容体の発現 (2008)
  • Author(s): 大石 亮, 舟場正幸, 村上 賢
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
• [Presentation] TGF-βによるBMP-regulated Smadのリン酸化とBMPによるTGF-β/activin-regulated Smadのリン酸化 (2008)
  • Author(s): 村上 賢, 小川健司, 舟場正幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市