| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Sexual dimorphic expression of IFNτ of bovine embryo was not caused via the sexual difference of expression of transcription factors, which affect IFNτ gene expression. However, the expression of Dnmt3a, one of DNA methyltransferases, of bovine male blastocysts was significantly higher than that of female embryos. Moreover, addition of 5-azadeoxycytidene, an inhibitor of DNA methyltransferase, to the culture medium of bovine embryo abolished the sexual dimorphic secretion of IFNτ from bovine embryos. These results suggest that the DNA methylation of bovine embryo genome might be involved in the occurrence of sexual dimorphic expression of IFNτ in bovine embryos.
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