| Project/Area Number |
20580341
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KUWAMURA Mitsuru Osaka Prefecture University, 生命環境科学研究科, 准教授 (20244668)
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| Co-Investigator(Kenkyū-buntansha) |
YAMATE Jyoji 大阪府立大学, 生命環境科学研究科, 教授 (50150115)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脱髄 / ミエリン / ミクログリア / オリゴデンドログリア / サイトカイン / グリア細胞 / ミエリン形成 / アトラクチン / モデル動物 |
|---|
| Research Abstract |
The dmy rat is an autosomal recessive mutant that exhibits severe myelin destruction. The number of oligodendrocytes decreases rapidly from 7weeks of age in the dmy rat. Hypertrophic oligodendrocytes were frequently observed, and the cytoplasm was found to be intensely positive for mitochondrial markers.
We found that a G-to-A transition, 177 bp downstream of exon 3 of the Mrs2 (MRS2 magnesium homeostasis factor (Saccharomyces cerevisiae)) gene.
We demonstrated an abnormal iron deposition, and significant upregulation of antioxidant enzyme heme oxygenase-1 (HO-1) and iron storage protein ferritin in the dmy rat.
Immunohistochemical and Western blot analyses demonstrated a considerable reduction in the expression of major CNS myelin proteins both in the white and gray matter of mv rats. However, there was no significant difference between control and mv rats in the cell number of PLP mRNA-positive oligodendrocytes either in the white or gray matter.
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