Pregnancy loss in high yielding dairy cows determined by the international standard protocols

• Project/Area Number: 20580350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: KATAGIRI Seiji Hokkaido University, 獣医学部, 教授 (00292061)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 臨床繁殖 / 産科 / 受胎性 / 胚死滅 / リピートブリーダー / 乳牛 / 子宮内膜 / 診断法 / 上皮成長因子 / 乳用牛 / リピートブリーディング / 妊娠 / 農場 / 国際共同研究 / 生検

Research Abstract

International standard protocols for diagnosis and research to determine causes of infertility in dairy cows were developed with co-investigators in EU and North American countries. A pilot survey using the standard protocols revealed potential difference in time and frequency of embryonic death between countries and/or regions. These results highlighted a few areas on which Japanese dairy industry needs to focus research activities to reduce pregnancy loss caused by embryonic death.

Research Products

• [Journal Article] Measurement of pO_2 in cultured mouse oocytes using electron paramagnetic resonance oximetry. (2010)
  • Author(s): Higaki S, Fujii H, Nagano M, Katagiri S, Takahashi Y.
  • Journal Title: Biomed Res 31 Pages: 165-168
  • Peer Reviewed
• [Journal Article] Productionof fertile zebrafish (Danio rerio) possessing germ cells (gametes) originated from priordial germ cells recovered from vitrified embryos. (2010)
  • Author(s): Higaki S, Eto Y, Kawakami Y, Yamaha E, Kagawa N, Kuwayama M, Nagano M, Katagiri S, Takahashi Y.
  • Journal Title: Reproduction 139 Pages: 733-740
  • Peer Reviewed
• [Journal Article] Cryopreservation of priomordial germ cells by rapid cooling of whole zebrafish (Danio rerio) embryos. (2010)
  • Author(s): Higaki S, Mochizuki K, Akashi Y, Yamaha E, Katagiri S, Takahashi Y.
  • Journal Title: J Reprod Dev 56 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Cryopreservation of primordial germ cells by rapid cooling of whole zebrafish (Danio reio) embryos (2010)
  • Author(s): Higaki, et al.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Production of fertile zebrafish (Danio rerio) possessing germ cells (gametes) originated from primordial germ cells recovered from vitrified embryo (2010)
  • Author(s): Higaki, et al.
  • Journal Title: Reproduction Volume: 139 Pages: 733-740
  • Peer Reviewed
• [Journal Article] Measurement of pO2 in cultured mouse oocytes using electron paramagnetic resonance oximetry (2010)
  • Author(s): Higaki, et al.
  • Journal Title: Biomedical Research Volume: 31 Pages: 165-168
  • Peer Reviewed
• [Journal Article] Feasibility of cryopreservation of zebrafish (Danio rerio) primordial germ cells by whole embryo freezing. (2009)
  • Author(s): Higaki S, Mochizuki K, Baba H, Akashi Y, Yamaha E, Katagiri S, Takahashi Y.
  • Journal Title: Jap J Vet Res 57 Pages: 119-128
  • NAID: 120001596867
  • Peer Reviewed
• [Journal Article] Fetal age estimation of Hokkaido Sika deer (cervus nippon yesoensis) using ultrasonography during early pregnancy. (2009)
  • Author(s): Yanagawa Y, Matsuura Y, Suzuki M, Saga S, Okuyama H, Fukui D, Bando G, Katagiri S, Takahashi Y, Tsubota T.
  • Journal Title: J Reprod Dev 55 Pages: 143-148
  • NAID: 10024941530
  • Peer Reviewed
• [Journal Article] 乳牛の分娩後の子宮回復および炎症性子宮疾患の分類 (2009)
  • Author(s): 片桐成二
  • Journal Title: 臨床獣医 27 Pages: 10-15
• [Journal Article] 牛の子宮内膜における上皮成長因子濃度と受胎性の関係-受精卵移植レシピエント牛の選抜への応用の可能性 (2009)
  • Author(s): 片桐成二
  • Journal Title: 家畜人工授精 255 Pages: 1-13
  • NAID: 40016956254
• [Journal Article] 牛の子宮内膜における上皮成長因子濃度と受胎性の関係 (2009)
  • Author(s): 片桐成二
  • Journal Title: 家畜人工授精 255 Pages: 1-13
• [Journal Article] A progestin-based treatment with a high dose of Estradiol benzoate normalizes cyclic changes in endometrial EGF concentrations and restores fertility in repeat breeder cows. (2008)
  • Author(s): Katagiri S, Takahashi Y.
  • Journal Title: J Reprod.Dev 54 Pages: 473-479
  • NAID: 10024940431
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of the estrogen receptor alpha (ERa) and progesterone receptor (PR) in the uterus of sika deer (cervus nippon) during pregnancy. (2008)
  • Author(s): Yanagawa Y, Matsuura Y, Suzuki M, Katagiri S, Tsubota T.
  • Journal Title: Jap J Vet Res 56 Pages: 139-149
  • Peer Reviewed
• [Journal Article] A progestin-based treatment with high dose of estradiol benzoate normalizes cyclic change of endometrial EGF concentrations and restores fertility in repeat breeder cows (2008)
  • Author(s): Katagiri S, Takahashi Y
  • Journal Title: Journal of Reproduction and Developlnent 54 Pages: 473-479
  • NAID: 10024940431
  • Peer Reviewed
• [Presentation] ホルスタイン種経産牛における合成型および天然型プロスタグランジン製剤が排卵卵胞、発情発現および排卵時期に及ぼす効果 (2011)
  • Author(s): 永井克尚、片桐成二, 他3名
  • Organizer: 第53回日本生殖医学会北海道地方部会学術講演会
  • Place of Presentation: 江別
  • Year and Date: 2011-02-19
• [Presentation] 牛の精漿による子宮内膜の免疫調節 (2011)
  • Author(s): 片桐成二
  • Organizer: 平成22年度北海道牛受精卵移植研究会講演会・シンポジウム
  • Place of Presentation: 札幌、招待講演
  • Year and Date: 2011-02-17
• [Presentation] 牛の精漿による子宮内膜の免疫調節 (2011)
  • Author(s): 片桐成二
  • Organizer: 平成22年度北海道牛受精卵移植研究会、シンポジウム「受胎・着床に関する新しい知見について」
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 2011-02-17
• [Presentation] 2種の腟内留置型プロジェステロン徐放剤を用いた定時胚移植プログラムにおける受胎率 (2011)
  • Author(s): 永井克尚、Gautam Gokarna、小坂啓太、安藤貴朗、森好政晴、片桐成二
  • Organizer: P4濃度変化および排卵後2日目の子宮内膜EGF濃度の検討日本獣医師会学会年次大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-02-12
• [Presentation] 2種の腟内留置型プロジェステロン徐放剤を用いた定時胚移植プログラムにおける受胎率、P4濃度変化および排卵後2日目の子宮内膜EGF濃度の検討 (2011)
  • Author(s): 永井克尚、片桐成二, 他4名
  • Organizer: 平成22年度日本産業動物獣医学会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-02-12
• [Presentation] Seminal plasma conteins protein to normalize the epidermal growth factor profile in the uterine endometrium and to restore fertility in repeat breeder cows. (2010)
  • Author(s): 片桐成二, 明見好信, 中田喜雄, 白澤篤, 金子絵美, 高橋芳幸
  • Organizer: XXVI World Buiatrics Congress(第26回世界牛病学会)
  • Place of Presentation: サンチアゴ、チリ
  • Year and Date: 2010-11-18
• [Presentation] Seminal plasma contains protein to normalize the epidermal growth factor profile in the uterine endometrium and to restore fertility in repeat breeder cows (2010)
  • Author(s): Katagiri, et al.
  • Organizer: XXVI World Buiatrics Congress第26回世界牛病学会
  • Place of Presentation: Santiago, Chile
  • Year and Date: 2010-11-18
• [Presentation] Effect of seminal plasma on uterine EGF profile and fertility in high yielding repeat breeder cows. (2010)
  • Author(s): 金子絵美, 白澤篤, 片桐成二, 明見好信, 中田喜雄, 高橋芳幸
  • Organizer: International Symposium on Declining Fertility in Dairy Cows in the World ; Its Causes and Possible Solutions.
  • Place of Presentation: 宮崎
  • Year and Date: 2010-01-31
• [Presentation] Effects of seminal plasma on uterine EGF profile and fertility in high yielding repeat breeder cows (2010)
  • Author(s): Emi Kaneko
  • Organizer: International Symposium for Declining Fertility in Dairy Cows in the World ; Its Causes and Possible Solutions
  • Place of Presentation: Miyazaki(宮崎)
  • Year and Date: 2010-01-31
• [Presentation] 乳用牛にみられる子宮内膜での上皮成長因子発現異常とその対策 (2009)
  • Author(s): 片桐成二
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良 招待講演
  • Year and Date: 2009-09-11
• [Presentation] 牛精漿による子宮内膜上皮成長因子の発現増強および受胎促進効果 (2009)
  • Author(s): 白澤篤、片桐成二、金子絵美、高見野枝、明見好信、中田喜雄、高橋芳幸
  • Place of Presentation: 奈良
  • Year and Date: 2009-09-11
• [Presentation] 乳用牛にみられる子宮内膜での上皮成長因子発現異常とその対策 (2009)
  • Author(s): 片桐成二
  • Organizer: 第102回日本繁殖生物学会大会(ワークショップ)
  • Place of Presentation: 奈良
  • Year and Date: 2009-09-11
• [Presentation] 牛精漿による子宮内膜上皮成長因子の発現増強および受胎促進効果 (2009)
  • Author(s): 白澤篤
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2009-09-11
• [Presentation] 牛子宮内膜における増殖因子発現調節による受胎性促進の試み (2009)
  • Author(s): 片桐成二
  • Organizer: 第28回北海道牛受精卵移植研究会
  • Place of Presentation: 札幌 招待講演
  • Year and Date: 2009-08-18
• [Presentation] 乳牛の子宮内膜における増殖因子の発現調節による受胎性促進の試み (2009)
  • Author(s): 片桐成二
  • Organizer: 第28回北海道牛受精卵移植研究会研究発表会(特別講演)
  • Place of Presentation: 札幌
  • Year and Date: 2009-08-18
• [Presentation] 牛における子宮内膜上皮成長因子(EGF)発現と受胎性の関係 (2009)
  • Author(s): 片桐成二
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 宇都宮 招待講演
  • Year and Date: 2009-04-02
• [Presentation] 牛における子宮内膜上皮成長因子(EGF)発現と受胎性の関係 (2009)
  • Author(s): 片桐成二
  • Organizer: 第147回日本獣医学会学術集会(シンポジウム)
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-02
• [Remarks] ホームページ等