Control of food poisoning using food and feed additives of chicken monoclonal antibodies.

• Project/Area Number: 20580357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: SASAI Kazumi Osaka Prefecture University, 生命環境科学研究科, 教授 (70211935)
• Co-Investigator(Kenkyū-buntansha): TANI Kiroyuki 大阪府立大学, 生命環境科学研究科, 准教授 (00305658) ; MATSUBAYASHI Makoto (独)農業・食品産業技術総合研究機構, 動物衛生研究所, 主任研究員 (00321076) ; FURUYA Masaru 大阪府立大学, 生命環境科学研究科, 助教 (30500706)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Apicomplexan parasites / リコンビナント抗体 / 感染防御 / 食品・飼料添加 / Apicomplexa / conoid / 鶏型モノクローナル抗体 / リコンビナント抗conoid抗体 / Cryptosporidium / Eimeria / ニワトリ型モノクローナル抗体 / apical complex

Research Abstract

In order to protect against Apicomplexan parasites infection, tried to prepare the chicken monoclonal antibodies specific for them. In FY 2008, the cross-reactivity of chicken monoclonal antibodies against Crptosporidium was confirmed. In FY 21, monoclonal antibodies against apical complex which had been reported to be involved in protozoa invasion of host cells had cross-reactivities agains Eimeria spicies, in the 2010 final, ScFv recombinant antibodies were produced and had the cross-reactivities agains Apicomplexan parasites.

Research Products

• [Journal Article] Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan. (2011)
  • Author(s): Matsubayashi M, Carreno RA, Tani H, Yoshiuchi R, Kanai T, Kimata I, Uni S, Furuya M, Sasai K.
  • Journal Title: Vet Parasitol. 176(2-3) Pages: 270-274
• [Journal Article] Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice. (2011)
  • Author(s): Matsubayashi M, Ando H, Kimata I, Takase H, Nakagawa H, Furuya M, Tani H, Sasai K.
  • Journal Title: Int J Parasitol. 41(3-4) Pages: 287-292
• [Journal Article] Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan. (2010)
  • Author(s): Yoshiuchi R, Matsubayashi M, Kimata I, Furuya M, Tani H, Sasai K.
  • Journal Title: Vet Parasitol. 174(3-4) Pages: 313-316
• [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media. (2010)
  • Author(s): Matsubayashi M, Ando H, Kimata I, Nakagawa H, Furuya M, Tani H, Sasai K.
  • Journal Title: Parasitology 137(13) Pages: 1861-1866
• [Journal Article] Characterization of monoclonal antibodies that recognize the Eimeria tenella microneme protein MIC2. (2008)
  • Author(s): Sasai K, Fetterer RH, Lillehoj H, Matusra S, Constantinoiu CC, Matsubayashi M, Tani H, Baba E.
  • Journal Title: J. Parasitol. 94 (6) Pages: 1432-1434
  • Peer Reviewed
• [Journal Article] Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan. (2008)
  • Author(s): Matsubayashi M, Nagano S, Kita T, Narushima T, Kimata I, Iseki M, Hajiri T, Tani H, Sasai K, Baba E.
  • Journal Title: Vet. Parasitol. 158 (1-2) Pages: 44-50
  • Peer Reviewed
• [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media
  • Author(s): M.Matsubayashi, H.Ando, I.Kimata, H.Nakagawa, M.Furuya, H.Tani, K.Sasai K
  • Journal Title: Parasitlogy (In press)
  • Peer Reviewed
• [Presentation] Molecular Characterization of the Novel Type of Cryptosporidium andersoni Detected in Japan. (2008)
  • Author(s): Matsubayashi M, Nagano S, Kita T, Narushima M, Kimata I, Iseki M, Hajiri M, Tani T, Sasai K, Baba E.
  • Organizer: Japanese-German Cooperative Symposium on Protozoan Disease.
  • Place of Presentation: Gottingen Germany
  • Year and Date: 2008-09-27
• [Remarks]
  • URL: http://www.vet.osakafu-u.ac.jp/int/int.html
• [Remarks]
  • URL: http://www.vet.osakafu-u.ac.jp/int/int.html