Expression analyses of canine UCP3 and development of a novel method for treatment of obesity in dogs.

• Project/Area Number: 20580358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: ISHIOKA Katsumi Nippon Veterinary and Life Science University, 獣医学部・獣医保健看護学科, 准教授 (60409258)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 内科 / 肥満 / イヌ / 骨格筋 / UCP3 / 不飽和脂肪酸

Research Abstract

Cell line developed from a canine tumor was proved to be myosarcoma and named "Canine Myogenic Sarcoma (CMS)" because cytokeratin, vimentin and desmin were positive, and MyoD1, myogenin and UCP3 mRNAs were expressed. When we cultured these cells with oleic acid, UCP3 expression was increased. UCP3 disseminates excess energy to heat, then it was suggested that oleic acid is effective for diet therapy of canine obesity.

Research Products

• [Journal Article] Establishment and characterization of canine rhabdomyosarcoma cell line, CMS-C. (2011)
  • Author(s): Azakami D., Shibutani H., Dohi M., Takasaki M., Ishioka K., Mori A., Momota Y., Bonkobara M., Washizu T., Michishita M., Hatakeyama H., Ogasawara S., Sako T.
  • Journal Title: Journal of Veterinary Medical Science (印刷中)
  • NAID: 130000677101
  • Peer Reviewed
• [Journal Article] Supplementing transglucosidase with a high-fiber diet for prevention of postprandial hyperglycemia in Streptozotocin-induced diabetic dogs. (2010)
  • Author(s): Sako, et al.
  • Journal Title: Veterinary Research Communications Volume: 34 Pages: 161-172
  • Peer Reviewed
• [Journal Article] Postprandial lipid-related metabolites are altered in dogs fed dietary diacylglycerol and low glycemic index starch during weight loss. (2010)
  • Author(s): Mitsuhashi, et al.
  • Journal Title: Journal of Nutrition Volume: 140 Pages: 1815-1821
  • Peer Reviewed
• [Journal Article] Feline adiponectin : molecular structures and plasma concentrations in obese cats. (2009)
  • Author(s): Ishioka, et al.
  • Journal Title: Journal of Veterinary Medical Science 71 Pages: 189-194
  • NAID: 110007109596
  • Peer Reviewed
• [Journal Article] Effect of glimepiride and nateglinide on serum insulin and glucose concentration in healthy cats. (2009)
  • Author(s): Mori, et al.
  • Journal Title: Veterinary Research Communications 33 Pages: 957-970
  • Peer Reviewed
• [Journal Article] 減量による肥満犬の糖代謝機能の改善 (2008)
  • Author(s): 大町麻子ら
  • Journal Title: ペット栄養学会誌 11 Pages: 13-19
  • Peer Reviewed
• [Presentation] 膀胱に発生した犬の横紋筋肉腫からの細胞株の樹立 (2010)
  • Author(s): 渋谷葉菜
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-06
• [Presentation] イヌUCP2およびUCP3のcDNAクローニングと発現解析 (2010)
  • Author(s): 宇田川智野
  • Organizer: 第12回日本ペット栄養学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-25
• [Presentation] 自然発生した犬の横紋筋肉腫由来の細胞株の樹立とその性状解析 (2009)
  • Author(s): 渋谷葉菜
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-26
• [Presentation] 伴侶動物におけるアディポカイン (2009)
  • Author(s): 石岡克己
  • Organizer: 第8回国際バイオフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2009-07-02
• [Presentation] ELISA法による猫のBCS別血中インスリン濃度の比較とその測定意義 (2009)
  • Author(s): 渡邉麻衣子
  • Organizer: 第147回日本獣医学会
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-02
• [Presentation] Low glycemic index starch plus diacylglycerol beneficially modifies obesity hormones during canine weight loss. (2008)
  • Author(s): Daisuke Nagaoka
  • Organizer: The 26th ACVIM Forum
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2008-06-06
• [Presentation] Adiponectin concentration does not correlate with obesity-associated changes in fasting insulin sensitivity in dogs. (2008)
  • Author(s): Verkest, K. R.
  • Organizer: The 26th ACVIM Forum
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2008-06-06
• [Book] 愛犬の病気診断 (2008)
  • Author(s): 江口陽子
  • Total Pages: 34
  • Publisher: 洋泉社