Analysis of denitrification activity expression factor of denitrification hotspot at groundwater environment based on molecular ecology

• Project/Area Number: 20580368
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: National Institute for Agro-Environmental Sciences
• Principal Investigator: NAKAJIMA Yasuhiro National Institute for Agro-Environmental Sciences, 物質循環研究領域, 主任研究員 (10354086)
• Co-Investigator(Kenkyū-buntansha): EGUCHI Sadao 独立行政法人農業環境技術研究所, 物質循環研究領域, 主任研究員 (30354020) ; HOSHINO Yuko 独立行政法人農業環境技術研究所, 生物生態機能研究領域, 研究員 (40354104) ; MORIMOTO Sho 独立行政法人農業環境技術研究所, 生物生態機能研究領域, 研究員 (00354118)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 硝酸態窒素 / 脱窒 / 深層土壌 / 地下水 / 分子生態学 / 微生物群集構造 / 水田 / 亜酸化窒素

Research Abstract

We developed a method of evaluating denitrification activity by analysis of nitrogen and oxygen stable isotope ratio of nitrate to search denitrification hotspot which has nitrate reduction activity.
We searched out denitrification hotspot from alluvium-diluvium interface and took subsoils. DNA was extracted from subsoil and PCR-DGGE profiles were made for analysis of denitrification hotspot-specific bacterial ecosystems. Subsoils near denitrification hotspot were incubated with nitrate-rich groundwater, results showed that nitrate was reduced within two weeks.

Research Products

• [Journal Article] 硝酸イオンの窒素・酸素安定同位体比測定のための安価な自動前処理ラインの開発 (2011)
  • Author(s): 高津文人
  • Journal Title: RADIOISOTOPES Volume: 60(印刷中)
  • Peer Reviewed
• [Journal Article] 窒素安定同位体比からみた笠岡湾干拓地内の水質汚濁解析 (2010)
  • Author(s): 前田守弘
  • Journal Title: 岡山大学異分野合先端研究コア年報 Pages: 71-71
• [Journal Article] Denitrification during vertical upwelling at an alluvium-diluvium interface below the upland perimeter of a riparian paddy (2009)
  • Author(s): Sadao Eguchi, Yasuhiro Nakajima, Shiho Yabusaki, Masahiro Kasuya, Hiroko Shibayama, Ayumi Tsunekawa, Katsuhiko Imai
  • Journal Title: J.Environ.Qual. 38 Pages: 2198-2209
  • Peer Reviewed
• [Journal Article] 安定同位体比を用いた干拓地内窒素動態の解析 (2009)
  • Author(s): 前田守弘
  • Journal Title: 岡山大学異分野融合先端研究コア年報 Pages: 87-87
• [Journal Article] nnual emissions of dissolved CO_2, CH_4, and N_2O in the subsurface drainage from three cropping systems (2009)
  • Author(s): Kazunori Minamikawa
  • Journal Title: Global Change Biology 16 Pages: 796-809
  • Peer Reviewed
• [Journal Article] Denitrification during vertical upwelling at an alluvium-diluvium interface below the upland perimeter of riparian paddy (2009)
  • Author(s): Sadao Eguchi
  • Journal Title: JOURNAL OF ENVIRONMENTAL QUALITY
  • Peer Reviewed
• [Journal Article] Annual emissions of dissolved CO_2, CH_4, and N_2O in the subsurface drainage from three cropping systems (2009)
  • Author(s): Kazunori Minamikawa
  • Journal Title: Global Change Biology (印刷中)
  • Peer Reviewed
• [Journal Article] モデルによる土壌,農耕地,流域における窒素動態の理解:3.地形連鎖系スケールの窒素動態:調査技法とモデル化手法 (2008)
  • Author(s): 江口定夫
  • Journal Title: 日本土壌肥料學雜誌 79 Pages: 213-227
  • NAID: 110007328358
  • Peer Reviewed
• [Journal Article] モデルによる土壌、農耕地、流域における窒素動態の理解3.地形連鎖系スケールの窒素動態-調査法とモデル化手法 (2008)
  • Author(s): 江口定夫
  • Journal Title: 日本土壌肥料学雑誌 79 Pages: 213-227
  • Peer Reviewed
• [Journal Article] 硝酸イオンの窒素・酸素安定同位体比測定のための安価な自動前処理ラインの開発
  • Author(s): 高津文人、今井章雄、中島泰弘、小松一弘、川崎伸之、佐藤貴則
  • Journal Title: RADIOISOTOPES (印刷中)
  • Peer Reviewed
• [Presentation] Analyzing nitrogen pollution in Kasaokabay reclaimed fields by using stable isotope natural abundances (2010)
  • Author(s): Morihiro Maeda
  • Organizer: 5th International Nitrogen Conference
  • Place of Presentation: ニューデリー
  • Year and Date: 2010-12-03
• [Presentation] 笠岡湾干拓地内にける水質汚染現状と安定同位体比による汚染源の推定 (2010)
  • Author(s): 浅野裕一
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-07
• [Presentation] 黒ボク土モノリスライシメータにおける浅い地下水面の有無がN2O発生に及ぼす影響 (2010)
  • Author(s): 南川和則
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-07
• [Presentation] 安定同位体比を用いた笠岡湾干拓地内窒素汚染の解析 (2010)
  • Author(s): 前田守弘
  • Organizer: 農業農村工学会
  • Place of Presentation: 神戸大学
  • Year and Date: 2010-08-31
• [Presentation] 地形連鎖系の浅層地下水帯を通じた脱窒に伴う硝酸性窒素濃度と窒素・酸素安定同位体比の変動(2) (2010)
  • Author(s): 中島泰弘
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道大学
• [Presentation] 地形連鎖系の浅層地下水帯を通じた脱窒に伴う硝酸性窒素濃度と窒素・酸素安定同位体比の変動 (2009)
  • Author(s): 中島泰弘
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学
• [Presentation] 窒菌を利用した硝酸態窒素の窒素および酸素安定同位体比の分析-分析法の簡易・迅速化および畑地-水田地形連鎖系における窒素動態解析への適用- (2008)
  • Author(s): 中島泰弘
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] 脱窒菌を利用した硝酸態窒素の窒素および酸素安定同位体比の分析分析法の簡易・迅速化および畑地-水田地形連鎖系における窒素動態解析への適用 (2008)
  • Author(s): 中島泰弘
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
• [Presentation] Denitrification in the shallow aquifer below the riparian paddy fields adjacent to the upland tea fields (2008)
  • Author(s): Sadao Eguchi
  • Organizer: Proceedings of the International Workshop in NSFC-JST strategic China-Japan Joint Research Program on "Comparative Study of Nitrogen Cycling and Its Impact on Water Quality in Agricultural Watersheds in Japan and China"
• [Presentation] Nitrate removal in the shallow aquifer below riparian paddy fields adjacent to upland tea fields (2008)
  • Author(s): Sadao EGUCHI
  • Organizer: Abstract of the XXXVI International Association of Hydrogeologistic Congress