Analysis for the mechanism of genome stability by the TREX complex, which has an essential role for the mRNA maturation in the nucleus.
| Project/Area Number |
20580370
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
MASUDA Seiji Kyoto University, 大学院・生命科学研究科, 准教授 (20260614)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MOMMA Keiko 京都文教大学, 人間学部, 准教授 (70397561)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 分子間相互作用 / mRNA / UAP56 / URH49 / TREX / AREX / ゲノム安定性 |
|---|
| Research Abstract |
The TREX complex has an essential function in mRNA biogenesis. Here I have studied the function of UAP56 and URH49, whish are the component of the TREX complex, in view of the relationship between mRNA biogenesis and genome stability. Fragmentation from chromosome DNA was observed when URH49 was knocked down rather than that UAP56 was. This phenotype was rescued by the forced expression of URH49, RNaseH, SF2 or RNPS1. These results suggest that the TREX complex functions to regulate the interaction of nascent RNA with genomic DNA to prevent the genome instability.
|
Report
(4 results)
Research Products
(21 results)
-
[Journal Article] The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression.2010
Author(s)
Yamazaki T., Fujiwara N., Yukinaga H., Ebisuya M., Shiki T., Kurihara T., Kioka N., Kambe K., Nagao M., Nishida E., Masuda S.
-
Journal Title
Mol. Biol. Cell. 21
Pages: 2953-2965
Related Report
Peer Reviewed
-
-
[Journal Article] The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression.2010
Author(s)
Yamazaki, T., Fujiwara, N., Yukinaga, H., Ebisuya, M., Shiki, T., Kurihara, T., Kioka, N., Kambe, K., Nagao, M., Nishida, E., Masuda S.
-
Journal Title
Mol.Biol.Cell
Volume: 21
Pages: 2953-2965
Related Report
Peer Reviewed
-
[Journal Article] A Screening Method Tuned for mRNA Processing Factors in Human Cells by Evaluating the Luciferase Reporter Activity and the Subcellular Distribution of Bulk Poly(A)^+ RNA.2010
Author(s)
Fujiwara, N., Yoshikawa, M., Yamazaki, T., Kambe, T., Nagao, M., Masuda, S.
-
Journal Title
Biosci.Biotechnol.Biochem.
Volume: 74
Pages: 1512-1516
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] 分子生物学2011
Author(s)
深見泰夫編集、分担執筆、3,4章担当
Total Pages
237
Publisher
化学同人
Related Report
-
