Structural biological studies on the sugar-binding mechanism of R-type lectin having a specific sugar-binding activity
| Project/Area Number |
20580373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
HEMMI Hikaru National Agricultural Research Organization, 食品分析研究領域, 主任研究員 (70353993)
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| Co-Investigator(Kenkyū-buntansha) |
KUNO Atsushi 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 主任研究員 (50302287)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 蛋白質 / 糖鎖 / NMR / レクチン / 糖認識ドメイン |
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| Research Abstract |
We investigated the sugar-binding mechanism of the C-terminal domain of R-type lectin from earthworm (EW29Ch) having a specific sugar-binding activity by nuclear magnetic resonance (NMR) methods. The results showed that the sugar-binding activity of the α binding site in EW29Ch was about 100-fold higher than that of the γ binding site in EW29Ch. Further, the NMR structure analysis and relaxation experiments for EW29Ch suggested that the difference of the sugar-binding activity between the two sugar-binding sites in EW29Ch may be related with the difference of the slow internal motion between those two sugar-binding sites in EW29Ch.
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Report
(4 results)
Research Products
(15 results)
