Structural biological studies on the sugar-binding mechanism of R-type lectin having a specific sugar-binding activity

• Project/Area Number: 20580373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: National Agricultural Research Organization
• Principal Investigator: HEMMI Hikaru National Agricultural Research Organization, 食品分析研究領域, 主任研究員 (70353993)
• Co-Investigator(Kenkyū-buntansha): KUNO Atsushi 独立行政法人産業技術総合研究所, 糖鎖医工学研究センター, 主任研究員 (50302287)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 蛋白質 / 糖鎖 / NMR / レクチン / 糖認識ドメイン

Research Abstract

We investigated the sugar-binding mechanism of the C-terminal domain of R-type lectin from earthworm (EW29Ch) having a specific sugar-binding activity by nuclear magnetic resonance (NMR) methods. The results showed that the sugar-binding activity of the α binding site in EW29Ch was about 100-fold higher than that of the γ binding site in EW29Ch. Further, the NMR structure analysis and relaxation experiments for EW29Ch suggested that the difference of the sugar-binding activity between the two sugar-binding sites in EW29Ch may be related with the difference of the slow internal motion between those two sugar-binding sites in EW29Ch.

Research Products

• [Journal Article] 核磁気共鳴(NMR)法を用いた立体構造解析及び分子間相互作用解析による有用タンパク質の機能解明 (2011)
  • Author(s): 逸見光
  • Journal Title: 食糧 49巻 Pages: 67-84
  • NAID: 40018848968
• [Journal Article] 核磁気共鳴(NMR)法を用いた立体構解析及び分子間相互作用解析による有用タンパク質の機能解明 (2011)
  • Author(s): 逸見光
  • Journal Title: 食糧 Volume: 49 Pages: 67-84
• [Journal Article] NMR studies on the interaction of sugars with C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris (2009)
  • Author(s): Hikaru Hemmi, Atsushi Kuno, Shigeyasu Ito, Ryuichiro Suzuki, Tsunemi Hasegawa, Jun Hirabayashi
  • Journal Title: FEBS Journal Vol.276 Pages: 2095-2105
  • Peer Reviewed
• [Journal Article] NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris (2009)
  • Author(s): 逸見光、久野敦, 他4名
  • Journal Title: FEBS Journal 276 Pages: 2095-2105
  • Peer Reviewed
• [Presentation] ミミズ由来R型レクチンC末端ドメインのラクトースとの結合状態でのNMR構造 (2010)
  • Author(s): 逸見光、久野敦、海野幸子、平林淳
  • Organizer: 第49回NMR討論会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2010-11-17
• [Presentation] NMR structure and dynamics of C-terminal domain of R-type lectin from earthworm in the lactose-binding state (2010)
  • Author(s): 逸見光、久野敦、海野幸子、平林淳
  • Organizer: 24th International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Cairns Convention Centre(オーストラリア)
  • Year and Date: 2010-08-25
• [Presentation] NMR structure and dynamics of C-terminal domain of R-type lectin from earthworm in the lactose-binding state (2010)
  • Author(s): 逸見光、久野敦, 他2名
  • Organizer: 24^<th> International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Cairns Convention Centre(オーストラリア)
  • Year and Date: 2010-08-25
• [Presentation] ミミズ由来R型レクチンC末端ドメインと糖との結合様式に関するNMR解析 (2009)
  • Author(s): 逸見光、久野敦、海野幸子、平林淳
  • Organizer: 第48回NMR討論会
  • Place of Presentation: 九州大学馬出病院キャンパス医学部百年講堂(福岡)
  • Year and Date: 2009-11-10
• [Presentation] NMR studies for the binding of lactose with C-terminal domain of an R-type lectin from the earthworm (2009)
  • Author(s): 逸見光、久野敦、海野幸子、平林淳
  • Organizer: The 3rd Asia-Pacific NMR Symposium
  • Place of Presentation: Ramada Plaza Jeju Hotel(韓国)
  • Year and Date: 2009-10-26
• [Presentation] NMR studies for the binding of lactose with the C-terminal domain of an R-type lectin from the earthworm (2009)
  • Author(s): 逸見光、久野敦, 他2名
  • Organizer: The 3^<rd> Asia-Pacific NMR Symposium
  • Place of Presentation: Ramada Plaza Jeju Hotel(韓国)
  • Year and Date: 2009-10-26
• [Presentation] NMRによるミミズ由来R 型レクチンC末端ドメインの特異的な糖結合活性に関する研究 (2008)
  • Author(s): 逸見光、久野敦、海野幸子、平林淳
  • Organizer: 第47回NMR討論会
  • Place of Presentation: 筑波大学大学会館講堂
  • Year and Date: 2008-11-14
• [Presentation] NMRによるミミズ由来R型レクチンC末端ドメインの特異的な糖結合活性に関する研究 (2008)
  • Author(s): 逸見光, 久野敦, 他2名
  • Organizer: 第47回NMR討論会
  • Place of Presentation: 筑波大学大学会館講堂
  • Year and Date: 2008-11-14
• [Presentation] NMR structure of C-terminal domain of R-type lectin from earthworm in the sugar-free state and its interaction with sugars (2008)
  • Author(s): 逸見光、久野敦、伊藤茂泰、鈴木龍一郎、長谷川典巳、平林淳
  • Organizer: 23rd International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Sheraton San Diego Hotel & Marina(アメリカ合衆国)
  • Year and Date: 2008-08-25
• [Presentation] NMR structure of C-terminal domain of R-type lectin from earthworm in the sugar-free state and its interaction with sugars (2008)
  • Author(s): 逸見光, 久野敦, 他4名
  • Organizer: 23^<rd> International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Sheraton San Diego Hotel & Marina (アメリカ合衆国)
  • Year and Date: 2008-08-25
• [Remarks] 逸見光、NMR によるR型レクチンC末端ドメイン糖結合部位の結合活性測定、独立行政法人農業・食品産業技術総合研究機構食品総合研究所平成21年度食品試験研究成果情報
  • URL: http://nfri.naro.affrc.go.jp/resear/seika/seika21/index.html