Estimation of in situ growth rate of harmful algae using cell cycle analysis
Project/Area Number |
20589002
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General fisheries
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Research Institution | Saga University |
Principal Investigator |
KATANO Toshiya Saga University, 低平地沿岸海域研究センター, 准教授 (00509820)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥3,180,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
Keywords | 赤潮 / 増殖速度 / プランクトン / 海洋保全 / 水産 / 海洋生態 / 日周鉛直移動 / 細胞分裂周期 / 細胞周期 / 赤潮原因藻類 / 細胞周期解析 / 細胞の固定方法 |
Research Abstract |
Fixation protocol has been developed for Chattonella and Heterosigma. We confirmed that this protocol can also apply to naked dinoflagellate of Akashiwo sanguinea. Andthe development of fixation protocol enabled us to analyze cell cycle of these red tide causatives. Using this protocol, field sampling had been carried out for Chattonella and Akashiwo in two hours interval for 24 hours. For Akashiwo sanguinea, we successfully analyzed their in situ growth rate.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Fixation of Chattonella antiqua, and C.marina (Raphidophyceae) using Hepes-buffered paraformaldehyde and glutaraldehyde for flow cytometry and light microscopy.2009
Author(s)
Katano, T., Yoshida, M., Lee, J., Han, M.-S., Hayami, Y.
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Journal Title
Phycologia 48
Pages: 473-479
Related Report
Peer Reviewed
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[Journal Article] Diel vertical migration and cell division of bloom-forming dinoflagellate Akashiwo sanguinea in the Ariake Sea, Japan.
Author(s)
Katano, T., Yoshida, M., Yamaguchi, S., Hamada, T., Yoshino, K., Hayami, Y.
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Journal Title
Plankton and Benthos Research (in press)
NAID
Related Report
Peer Reviewed
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