Estimation of in situ growth rate of harmful algae using cell cycle analysis

• Project/Area Number: 20589002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Saga University
• Principal Investigator: KATANO Toshiya Saga University, 低平地沿岸海域研究センター, 准教授 (00509820)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,180,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 赤潮 / 増殖速度 / プランクトン / 海洋保全 / 水産 / 海洋生態 / 日周鉛直移動 / 細胞分裂周期 / 細胞周期 / 赤潮原因藻類 / 細胞周期解析 / 細胞の固定方法

Research Abstract

Fixation protocol has been developed for Chattonella and Heterosigma. We confirmed that this protocol can also apply to naked dinoflagellate of Akashiwo sanguinea. Andthe development of fixation protocol enabled us to analyze cell cycle of these red tide causatives. Using this protocol, field sampling had been carried out for Chattonella and Akashiwo in two hours interval for 24 hours. For Akashiwo sanguinea, we successfully analyzed their in situ growth rate.

Research Products

• [Journal Article] Diel vertical migration and cell division of bloom-forming dinoflagellate Akashiwo sanguinea in the Ariake Sea, Japan (2011)
  • Author(s): Katano, et al.
  • Journal Title: Plankton and Benthos Research Volume: (印刷中)
  • NAID: 10029655355
  • Peer Reviewed
• [Journal Article] Effects of temperature, salinity, and prey organisms on the growth of three Pfiesteria-like heterotrophic dinoflagellates. (2010)
  • Author(s): Baek, S.H., You, K., Katano, T., Shin, K.
  • Journal Title: Plankton and Benthos Research corresponding author 5 Pages: 31-38
  • NAID: 10026447508
  • Peer Reviewed
• [Journal Article] Effects of temperature, salinity, and prey organisms on the growth of three Pfiesteria-like heterotrophic dinoflagellates (2010)
  • Author(s): Seung Ho Baek, Kai You, Toshiya Katano, Kyoungsoon Shin
  • Journal Title: Plankton and Benthos Research 5 Pages: 31-38
  • NAID: 10026447508
  • Peer Reviewed
• [Journal Article] Fixation of Chattonella antiqua, and C.marina (Raphidophyceae) using Hepes-buffered paraformaldehyde and glutaraldehyde for flow cytometry and light microscopy. (2009)
  • Author(s): Katano, T., Yoshida, M., Lee, J., Han, M.-S., Hayami, Y.
  • Journal Title: Phycologia 48 Pages: 473-479
  • Peer Reviewed
• [Journal Article] Fixation of Chattonella antiqua and C. marina (Raphidophyceae) using Hepes-buffered paraformaldehyde and glutaraldehyde for flow cytometry and lightmicroscopy (2009)
  • Author(s): Toshiya Katano, Makoto Yoshida, Juyun Lee, Myung-Soo Han, Yuichi Hayami
  • Journal Title: Phycologia (in press)
  • Peer Reviewed
• [Journal Article] Diel vertical migration and cell division of bloom-forming dinoflagellate Akashiwo sanguinea in the Ariake Sea, Japan.
  • Author(s): Katano, T., Yoshida, M., Yamaguchi, S., Hamada, T., Yoshino, K., Hayami, Y.
  • Journal Title: Plankton and Benthos Research (in press)
  • NAID: 10029655355
  • Peer Reviewed
• [Presentation] 河川からの出水直後のラフィド藻Chattonellaのブルーム (2011)
  • Author(s): 片野俊也、吉野健児、松原賢、速水祐一
  • Organizer: 日本海洋学会(みなし開催)
  • Place of Presentation: 千葉県柏市
  • Year and Date: 2011-03-23
• [Presentation] 有明海における渦鞭毛藻Akashiwo sanguineaの細胞分裂頻度からの増殖速度の見積り (2010)
  • Author(s): 片野俊也、吉田誠、山口創一、濱田孝治、吉野健児、速水祐一
  • Organizer: 日本プランクトン・ベントス学会
  • Place of Presentation: 千葉県柏市
  • Year and Date: 2010-10-10
• [Presentation] ラフィド藻Chattonellaの日周鉛直移動 (2010)
  • Author(s): 片野俊也、吉田誠、山口創一、速水祐一
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2010-03-27
• [Presentation] ラフィド藻Chattonellaの日周鉛直移動 (2010)
  • Author(s): 片野俊也、吉田誠、山口創一、速水祐一
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京都品川区
• [Presentation] Heterosigma akashiwoの細胞周期解析:細胞分裂のタイミングと日周鉛直移動の関係 (2008)
  • Author(s): Lee Juyun,片野俊也、速水祐一、Han Myung-Soo
  • Organizer: 日本海洋学会
  • Place of Presentation: 広島県呉市
  • Year and Date: 2008-09-25
• [Presentation] Heterosigma akashiwoの細胞周期解析 : 細胞分裂のタイミングと日周鉛直移動の関係 (2008)
  • Author(s): Lee Juyun, 片野俊也、速水祐一、Han Myung-Soo
  • Organizer: 日本海洋学会秋季大会
  • Place of Presentation: 広島県呉市
  • Year and Date: 2008-09-25
• [Remarks] ホームページ等 なし