| Budget Amount *help |
¥3,310,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
Proteins from hyperthermophiles contain a lot of ionic residues compared to those from other sources growing in moderate environments. However, the contribution of ionic interaction to thermo-stability of the proteins remains to be clearly understood. The CutA1 protein from hyperthermophile, Pyrococcus horikoshii (PhCutA1) shows the highest denaturation temperature of nearly 150 oC. In this report, we investigated the thermo-stabilities of more than 50 PhCutA1 mutants (from ionic to non-ionic residues) to elucidate the contribution of the ionic interaction.
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