Construction of influenza virosome and its development to preparation of vaccine

• Project/Area Number: 20590033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: University of Toyama
• Principal Investigator: UENO Masaharu University of Toyama, 大学院・医学薬学研究部(薬学), 教授 (40080197)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 製剤学 / インフルエンザ / ビロソーム / 人工膜ワクチン / オレイン酸 / ラフト / HA / NA / 中和抗体 / 皮下投与 / 経鼻投与 / アジュバント / BALF / インフルエンザビロソーム / ウイルス抗原蛋白質 / ELISA / フリーズ・フラクチャー / 電子顕微鏡

Research Abstract

Influenza is one of the common infectious diseases caused by influenza virus. Vaccines are the most effective means to fight infectious diseases. Liposomes are known to be effective immunoadjuvants. In this article, we introduced a new method to prepare the liposome vaccine, "influenza virosomes", using inter-membrane protein transfer. We concluded that this method is useful to prepare virosomes. The virosomes showed high immunoactivity especially with MDP derivatives.

Research Products

• [Presentation] オレイン酸のベシクル形成 (2010)
  • Author(s): 吉田湖南、桜田剛浩、茶木弘一、S.Chungcharoenwattana、奥野貴士、上野雅晴
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] Preparation of Influenza Virosomes using Inter-Membrane Protein Transfer (2010)
  • Author(s): 上野雅晴, 奥野貴士, 林京子
  • Organizer: International Liposome Research Days & Lipid, Liposomes & Membrane Biophysics
  • Place of Presentation: Vancouver
• [Presentation] Vesicle Formation of Fatty Acid in the Presence of Preformed Phospholipid Vesicles (2010)
  • Author(s): Masaharu Ueno, S.Chngcharoenwattana, Takashi Okuno, Konan Yoshida
  • Organizer: PACIFICHEM
  • Place of Presentation: Honolulu
• [Presentation] Preparation of Influenza Virosomes using Inter-membrane Protein Transfer (2010)
  • Author(s): Masaharu Ueno, Takashi Okuno, Kyoko Hayashi
  • Organizer: Internitional Liposome Research Days & Lipid, Liposomes & Membrane Biophysics
  • Place of Presentation: Vancouver
• [Presentation] Vesicle Formation of Fatty Acid in the Presence of Preformed Phospholipid Vesicles (2010)
  • Author(s): Masaharu Ueno, Supapon Chungcharoen Wattana, Takashi Okuno, Konan Yoshida
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu
• [Presentation] オレイン酸のベシクル形成 (2010)
  • Author(s): 吉田湖南、桜田剛浩、茶木弘一、S.Chungcharoenwattana、奥野貴士、上野雅晴
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山(岡山大学)
• [Presentation] 膜蛋白質の膜間移行を利用したインフルエンザ人工膜ワクチンの試作 (2009)
  • Author(s): 白石智子、木平孝高、林京子、奥野貴士、上野雅晴
  • Organizer: 日本薬学会代129年会
  • Place of Presentation: 京都
• [Presentation] インフルエンザビロソームの構築 (2009)
  • Author(s): 萩原由希子、白石智子、林京子、奥野貴士、上野雅晴
  • Organizer: 日本膜学会代31年会
  • Place of Presentation: 東京
• [Presentation] インフルエンザビロソームの構築 (2009)
  • Author(s): 萩原由希子、白石智子、林京子、奥野貴士、上野雅晴
  • Organizer: 日本膜学会第31年会
  • Place of Presentation: 東京(東京理科大学)
• [Presentation] 膜蛋白質の膜間移行を利用した人工膜ワクチンの試作 (2009)
  • Author(s): 白石智子, 木平孝高, 林京子 奥野貴士, 上野雅晴
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都(国際会議場)