| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Research Abstract |
Universal type of high-speed countercurrent chromatograph (universal HSCCC) was newly designed and fabricated in our university, and applied to protein separation using an aqueous-aqueous polymer phase system. The present apparatus can produce a synchronous planetary motion of coiled separation column by selecting the normal forward rotation (P_<forward>) or the reversed backward rotation (P_<backward>) against the revolution direction. No stationary phase retention was obtained in P_<backward> rotation, while sufficient protein separation was achieved in P_<forward> rotation. In this P_<forward> rotation, partition efficiency obtained by the eccentric coil assembly was better than that obtained by the toroidal coil assembly. By improving the apparatus to the nonsynchronous HSCCC, which can produce different rotation and revolution speeds independently, increased partition efficiency in P_<forward> rotation was obtained more than the original universal HSCCC. The nonsynchronous HSCCC was successfully fabricated by introducing the new rotary seal system in the distribution line of flow tube.
|
