Development of novel monitoring system for quorum sensing mediators in Gram-negative bacteria

• Project/Area Number: 20590041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Nihon University
• Principal Investigator: MIYAIRI Shinichi Nihon University, 薬学部, 教授 (50209855)
• Co-Investigator(Kenkyū-buntansha): SAITO Hiroaki 日本大学, 薬学部, 助教 (30385976)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: グラム陰性菌 / 緑膿菌 / クオラムセンシング / オートインデューサー / 高速液体クロマトグラフィー / 蛍光誘導体化 / エラスターゼ活性測定法 / 蛍光標識化試薬 / エラスターゼ / ピオシアニン / マクロライド系抗生物質 / 毒性発現因子 / エラスターゼ合成基質 / ELISA

Research Abstract

In this project we have developed a convenient and simultaneous determination system of 3-oxo-acyl-HSLs, those are autoinducer molecules of Gram-negative bacteria, by high-performance liquid chromatography with fluorescence derivatization. Then we have clarified the relation of AI concentration, elastase activity and cell density using Pseudomonas aeruginosa, one of Gram-negative bacteria. The results are that AI concentration was well synchronized with cell growth and there were 2 hours-time lag between increasing of AI concentration and that of elastase activity.

Research Products

• [Journal Article] Roles of Pseudomonas aeruginosa autoinducers and their degradation products, tetramic acids, in bacterial survival and behavior in ecological niches. (2011)
  • Author(s): N.Hosono, S.Kimura, K.Tateda, M.Horikawa, C.Ueda, Y.Ishii, M.Ishiguro, S.Miyairi, K.Yamaguchi
  • Journal Title: Microbes and Environments
  • NAID: 10029122954
  • Peer Reviewed
• [Journal Article] Roles of Pseudomonas aeruginosa Autoinducers and their Degradation Products, Tetramic acids, in Bacterial Survival and Behavior in Ecological Niches. (2011)
  • Author(s): N.Hosono, S.Kimura K.Tateda, M.Horikawa, C.Ueda, Y.Ishii, M.Ishiguro, S.Miyairi, K.Yamaguchi
  • Journal Title: Microbes and Environments Volume: (Web公開3月29日)
  • NAID: 10029122954
  • Peer Reviewed
• [Journal Article] Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species. (2009)
  • Author(s): S.Kimura, K.Tateda, Y.Ishii, M. Horikawa, S.Miyairi, N.Gotoh, M.Ishiguro, K.Yamaguchi
  • Journal Title: Microbiology 155 Pages: 1934-1939
  • Peer Reviewed
• [Journal Article] Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species (2009)
  • Author(s): S.Kimura, K.Tateda, Y.Ishii, M.Horikawa, S.Miyairi, N.Gotoh, M.Ishiguro, K.Yamaguchi
  • Journal Title: Microbiology 155 Pages: 1934-1939
  • Peer Reviewed
• [Presentation] 緑膿菌のクオラムセンシング機構研究のための発蛍光性プローブの開発 (2011)
  • Author(s): 横田康男、小倉知佳、三上泰輝、市丸嘉、齋藤弘明、内山武人、宮入伸一
  • Organizer: 日本薬学会第131年会
  • Year and Date: 2011-03-31
• [Presentation] グラム陰性菌のオートインデューサーである3-オキソ脂肪酸のホモセリンラクトン誘導体の一斉分析法の開発 (2010)
  • Author(s): 三上泰輝、齋藤弘明、横田康男、三宅宗晴、内山武人、高畠亨、宮入伸一
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] グラム陰性菌のオートインデューサーである3-オキソ脂肪酸のホモセリンラクトン誘導体の一斉分析法の開発 (2010)
  • Author(s): 三上泰輝, 齋藤弘明, 横田康男, 三宅宗晴, 内山武人, 高畠亨, 宮入伸一
  • Organizer: 日本薬学会第130年回
  • Place of Presentation: 桃太郎アリーナ(岡山市)
  • Year and Date: 2010-03-29
• [Presentation] Inhibition of Pseudomonas aeruginosa quorum sensing system by acyl HSL analogs (2009)
  • Author(s): 齋藤弘明、宮入伸一
  • Organizer: 第25回内藤コンファレンス「ケミカルバイオロジー[II]」
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-10
• [Remarks] ホームページ等
  • URL: http://www.pha.nihon-u.ac.jp/page.jsp?id=1391
• [Remarks]
  • URL: http://www.pha.nihon-u.ac.jp/page.jsp?id=1391